Hyperthermia: Cancer Treatment and Beyond

Bettaieb, Ahmed; Wrzal, Paulina K.; Averill‐Bates, Diana A.

doi:10.5772/55795

Cited by 65 publications

(64 citation statements)

References 147 publications

(163 reference statements)

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“…37 Dex-LSMO nanoparticles-mediated hyperthermia resulted in HSP induction in tumors as compared to controls. In agreement with our results, Ito et al 38 reported that intra-tumorally injected iron oxide nanoparticles when exposed to alternating magnetic field for 30 minutes could induce cytoplasmic HSP70 immediately after treatment.…”

Section: Discussionmentioning

confidence: 99%

Hyperthermia mediated by dextran-coated La0.7Sr0.3MnO3 nanoparticles: in vivo studies

Haghniaz

Umrani

Paknikar

2016

IJN

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Purpose The aim of this study was to evaluate radiofrequency-induced dextran-coated lanthanum strontium manganese oxide nanoparticles-mediated hyperthermia to be used for tumor regression in mice. Materials and methods Nanoparticles were injected intra-tumorally in melanoma-bearing C57BL/6J mice and were subjected to radiofrequency treatment. Results Hyperthermia treatment significantly inhibited tumor growth (~84%), increased survival (~50%), and reduced tumor proliferation in mice. Histopathological examination demonstrated immense cell death in treated tumors. DNA fragmentation, increased terminal deoxynucleotidyl transferase-dUTP nick end labeling signal, and elevated levels of caspase-3 and caspase-6 suggested apoptotic cell death. Enhanced catalase activity suggested reactive oxygen species-mediated cell death. Enhanced expression of heat shock proteins 70 and 90 in treated tumors suggested the possible development of “antitumor immunity”. Conclusion The dextran-coated lanthanum strontium manganese oxide-mediated hyperthermia can be used for the treatment of cancer.

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Section: Discussionmentioning

confidence: 99%

Hyperthermia mediated by dextran-coated La0.7Sr0.3MnO3 nanoparticles: in vivo studies

Haghniaz

Umrani

Paknikar

2016

IJN

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“…Our proposed mechanism for this chemosensitization is that the heat-induced increase in membrane permeability caused by PTT enables more dox to be taken up and retained by the cells (Figure 3A), resulting in increased cytotoxic effects at doses that would ordinarily be subtherapeutic. [36][37][38] This mechanism is supported by the 56% increase in rhodamine fluorescence observed inside cells following PTT ( Figure 3B) and the 20% increase in dox fluorescence observed inside cells following PTT (Figure 4). Further, we have demonstrated that the 20% increase in intracellular dox localizes to the nucleus to effectively induce cytotoxicity.…”

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confidence: 67%

Nanoshell-mediated photothermal therapy can enhance chemotherapy in inflammatory breast cancer cells

Fay¹,

Melamed²,

Day³

2015

IJN

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Nanoshell-mediated photothermal therapy (PTT) is currently being investigated as a standalone therapy for the treatment of cancer. The cellular effects of PTT include loss of membrane integrity, so we hypothesized that nanoshell-mediated PTT could potentiate the cytotoxicity of chemotherapy by improving drug accumulation in cancer cells. In this work, we validated our hypothesis using doxorubicin as a model drug and SUM149 inflammatory breast cancer cells as a model cancer subtype. In initial studies, SUM149 cells were exposed to nano-shells and near-infrared light and then stained with ethidium homodimer-1, which is excluded from cells with an intact plasma membrane. The results confirmed that nanoshell-mediated PTT could increase membrane permeability in SUM149 cells. In complementary experiments, SUM149 cells treated with nanoshells, near-infrared light, or a combination of the two to yield low-dose PTT were exposed to fluorescent rhodamine 123. Analyzing rhodamine 123 fluorescence in cells via flow cytometry confirmed that increased membrane permeability caused by PTT could enhance drug accumulation in cells. This was validated using fluorescence microscopy to assess intracellular distribution of doxorubicin. In succeeding experiments, SUM149 cells were exposed to subtherapeutic levels of doxorubicin, low-dose PTT, or a combination of the two treatments to determine whether the additional drug uptake induced by PTT is sufficient to enhance cell death. Analysis revealed minimal loss of viability relative to controls in cells exposed to subtherapeutic levels of doxorubicin, 15% loss of viability in cells exposed to low-dose PTT, and 35% loss of viability in cells exposed to combination therapy. These data indicate that nanoshell-mediated PTT is a viable strategy to potentiate the effects of chemotherapy and warrant further investigation of this approach using other drugs and cancer subtypes.

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“…If we wish to improve this situation, it is necessary to better understand the effects of hyperthermia at the cellular level. It is well understood generally that heat leads to the denaturation of proteins, the loss of their function, the failure of some cellular pathways and the activation of others [1]. The end result is some probability of cell death that is reasonably characterized by the idea of a “thermal dose”, based on the thermal physics controlling denaturation of a generalized protein or set of proteins.…”

Section: Introductionmentioning

confidence: 99%

Non-lethal heat treatment of cells results in reduction of tumor initiation and metastatic potential

Kim

Lee

O’Neill

2015

Biochemical and Biophysical Research Communications

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Non-lethal hyperthermia is used clinically as adjuvant treatment to radiation, with mixed results. Denaturation of protein during hyperthermia treatment is expected to synergize with radiation damage to cause cell cycle arrest and apoptosis. Alternatively, hyperthermia is known to cause tissue level changes in blood flow, increasing the oxygenation and radiosensitivity of often hypoxic tumors. In this study, we elucidate a third possibility, that hyperthermia alters cellular adhesion and mechanotransduction, with particular impact on the cancer stem cell population. We demonstrate that cell heating results in a robust but temporary loss of cancer cell aggressiveness and metastatic potential in mouse models. In vitro, this heating results in a temporary loss in cell mobility, adhesion, and proliferation. Our hypothesis is that the loss of cellular adhesion results in suppression of cancer stem cells and loss of tumor virulence and metastatic potential. Our study suggests that the metastatic potential of cancer is particularly reduced by the effects of heat on cellular adhesion and mechanotransduction. If true, this could help explain both the successes and failures of clinical hyperthermia, and suggest ways to target treatments to those who would most benefit.

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Hyperthermia: Cancer Treatment and Beyond

Cited by 65 publications

References 147 publications

Hyperthermia mediated by dextran-coated La0.7Sr0.3MnO3 nanoparticles: in vivo studies

Hyperthermia mediated by dextran-coated La0.7Sr0.3MnO3 nanoparticles: in vivo studies

Nanoshell-mediated photothermal therapy can enhance chemotherapy in inflammatory breast cancer cells

Non-lethal heat treatment of cells results in reduction of tumor initiation and metastatic potential

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