Hyperspectral imaging for monitoring of perfusion failure upon microvascular anastomosis in the rat hind limb

Grambow, Eberhard; Dau, Michael; Holmer, Amadeus; Lipp, Vicky; Frerich, Bernhard; Klar, E.; Vollmar, Brigitte; Kämmerer, Peer W.

doi:10.1016/j.mvr.2017.10.005

Cited by 29 publications

(30 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Hence, there is an increasing demand for an objective, reliable, and investigator-independent method to assess flap perfusion. Hyperspectral imaging (HSI) could be suitable for clinical use as it is a non-contact, non-ionising, and noninvasive monitoring technique that provides objective, reproducible, precise, and relevant information about physiological parameters in different medical fields of application like tissue perfusion measurements and wound assessment [8][9][10]. HSI processes the optical properties of a large area in a wavelength range from visual light (approx.…”

Section: Introductionmentioning

confidence: 99%

Hyperspectral analysis for perioperative perfusion monitoring—a clinical feasibility study on free and pedicled flaps

Thiem¹,

Frick²,

Goetze³

et al. 2020

Clin Oral Invest

View full text Add to dashboard Cite

Objectives In reconstructive surgery, flap monitoring is crucial for early identification of perfusion problems. Using hyperspectral imaging (HSI), this clinical study aimed to develop a non-invasive, objective approach for perfusion monitoring of free and pedicled flaps. Material and methods HSI of 22 free (FF) and 8 pedicled flaps (PF) in 30 patients was recorded over time. Parameters assessed were tissue oxygenation/superficial perfusion (0–1 mm) (StO2 (0–100%)), near-infrared perfusion/deep perfusion (0–4 mm) (NIR (0–100)), distribution of haemoglobin (THI (0–100)), and water (TWI (0–100)). Measurements up to 72 h were correlated to clinical assessment. Results Directly after flap inset, mean StO2 was significantly higher in FF (70.3 ± 13.6%) compared with PF 56.2 ± 14.2% (p = 0.05), whereas NIR, THI, and TWI were similar (NIR_p = 0.82, THI_p = 0.97, TWI_p = 0.27). After 24 h, StO2, NIR, THI, and TWI did not differ between FF and PF. After 48 h, StO2, NIR, and TWI did not differ between FF and PF whereas THI was significantly increased in FF compared with PF(p = 0.001). In three FF, perfusion decreased clinically and in HSI, 36(1), 40(2), 5(3), and 61(3) h after flap inset which was followed by prompt intervention. Conclusions StO2 < 40%, NIR < 25/100, and THI < 40/100 indicated arterial occlusion, whereas venous problems revealed an increase of THI. In comparison with FF, perfusion parameters of PF were decreased after flap transfer but remained similar to FF later on. Clinical relevance HSI provides objective and non-invasive perfusion monitoring after flap transplantation in accordance to the clinical situation. With HSI, signs of deterioration can be detected hours before clinical diagnosis.

show abstract

Section: Introductionmentioning

confidence: 99%

Hyperspectral analysis for perioperative perfusion monitoring—a clinical feasibility study on free and pedicled flaps

Thiem¹,

Frick²,

Goetze³

et al. 2020

Clin Oral Invest

View full text Add to dashboard Cite

show abstract

“…Examples of HSI usability within (bio)medical disciplines range from perioperative support with guidance of the surgeon to delineate the right resection margins of lentigo maligna or cerebral neoplasms to assessing diabetic foot ulcer development risk. [2][3][4][5][6] Other proof-of-concepts measured the oxygen saturation (OS) of various organs 7 ; assessed the presence of molecules such as cholesterol, melanin, and hemoglobin 8,9 ; enhanced the surgeon's vision in oncologic surgery and laparoscopy [10][11][12][13] ; predicted hemorrhagic shock and appraising hemodynamics [14][15][16] ; classified corneal injury 17 ; augmented contrast for histologic examinations 18,19 ; and detected neoplasms of the skin, mouth, colon, brain, and others. 3,5,[20][21][22] In ophthalmology, HSI can be used to assess the state and distribution of chromophores, such as cytochrome C, and assess the metabolic status of hemoglobin in the context of retinal blood vessel oxygenation.…”

Section: Introductionmentioning

confidence: 99%

Hyperspectral Imaging and the Retina: Worth the Wave?

Lemmens

Eijgen

Keer

et al. 2020

Trans. Vis. Sci. Tech.

View full text Add to dashboard Cite

Purpose: Hyperspectral imaging is gaining attention in the biomedical field because it generates additional spectral information to study physiological and clinical processes. Several technologies have been described; however an independent, systematic literature overview is lacking, especially in the field of ophthalmology. This investigation is the first to systematically overview scientific literature specifically regarding retinal hyperspectral imaging. Methods: A systematic literature review was conducted, in accordance with PRISMA Statement 2009 criteria, in four bibliographic databases: Medline, Embase, Cochrane Database of Systematic Reviews, and Web of Science. Results: Fifty-six articles were found that meet the review criteria. A range of techniques was reported: Fourier analysis, liquid crystal tunable filters, tunable laser sources, dualslit monochromators, dispersive prisms and gratings, computed tomography, fiber optics, and Fabry-Perrot cavity filter covered complementary metal oxide semiconductor. We present a narrative synthesis and summary tables of findings of the included articles, because methodologic heterogeneity and diverse research topics prevented a meta-analysis being conducted. Conclusions: Application in ophthalmology is still in its infancy. Most previous experiments have been performed in the field of retinal oximetry, providing valuable information in the diagnosis and monitoring of various ocular diseases. To date, none of these applications have graduated to clinical practice owing to the lack of sufficiently large validation studies. Translational Relevance: Given the promising results that smaller studies show for hyperspectral imaging (e.g., in Alzheimer's disease), advanced research in larger validation studies is warranted to determine its true clinical potential.

show abstract

“…HSI combines spectroscopy and conventional imaging in order to determine the entire infrared spectra at different locations on an object, [ 43 ] and has found application in biotechnology [ 44 ] and medicine. [ 45 ] Mid‐IR HSI was carried out on the cross‐section of sealed tissue from mouse skin placed on a mirror (as a back reflector) in order to obtain insights into the characteristics of the tissue‐biosealant interface ( Figure a). Differences between the reflection spectra of the tissue and silk thin films can be seen in Figure 7b.…”

Section: Resultsmentioning

confidence: 99%

Chromophore‐Free Sealing and Repair of Soft Tissues Using Mid‐Infrared Light‐Activated Biosealants

Ridha

Basiri

Godeshala

et al. 2020

Adv Funct Materials

View full text Add to dashboard Cite

Poor strength, infection, leakage, long procedure times, and inflammation limit the efficacy of common tissue sealing devices in surgeries and trauma. Light‐activated sealing is attractive for tissue sealing and repair, and can be facilitated by the generation of local heat following absorption of nonionizing laser energy by chromophores. Here, the inherent ability of biomaterials is exploited to absorb nonionizing, mid‐infrared (midIR) light in order to engender rapid photothermal sealing and repair of soft tissue wounds. In this approach, the biomaterial simultaneously acts as a photothermal convertor as well as a biosealant, which dispenses the need for exogeneous light‐absorbing nanoparticles or dyes. Biomechanical recovery, mathematical modeling, histopathology analyses, tissue strain mapping using digital imaging correlation, and visualization of the biosealant‐tissue interface using hyperspectral imaging indicate superior performance of midIR sealing in live mice compared to conventional sutures and glue. The midIR‐biosealant approach demonstrates rapid sealing of soft tissues, improves cosmesis, lowers potential for scarring, obviates safety concerns because of the nonionizing light used, and allows adoption of a wide diversity of biomaterials. Taken together, the studies demonstrate a novel advance both in biomaterials for surgical sealing along with the use of nonionizing midIR light, with high potential for clinical translation.

show abstract

Hyperspectral imaging for monitoring of perfusion failure upon microvascular anastomosis in the rat hind limb

Cited by 29 publications

References 17 publications

Hyperspectral analysis for perioperative perfusion monitoring—a clinical feasibility study on free and pedicled flaps

Hyperspectral analysis for perioperative perfusion monitoring—a clinical feasibility study on free and pedicled flaps

Hyperspectral Imaging and the Retina: Worth the Wave?

Chromophore‐Free Sealing and Repair of Soft Tissues Using Mid‐Infrared Light‐Activated Biosealants

Contact Info

Product

Resources

About