Hyperspectral imaging and dynamic region of interest tracking approaches to quantify localized cAMP signals
Santina C. Johnson,
Naga S. Annamdevula,
Silas J. Leavesley
et al.
Abstract:Cyclic adenosine monophosphate (cAMP) is a ubiquitous second messenger known to orchestrate a myriad of cellular functions over a wide range of timescales. In the last 20 years, a variety of single-cell sensors have been developed to measure second messenger signals including cAMP, Ca2+, and the balance of kinase and phosphatase activities. These sensors utilize changes in fluorescence emission of an individual fluorophore or Förster resonance energy transfer (FRET) to detect changes in second messenger concen… Show more
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