Hyperspectral Imaging: A New Approach to the Diagnosis of Hemorrhagic Shock

Cancio, Leopoldo C.; Batchinsky, Andriy I.; Mansfield, James R.; Panasyuk, Svetlana; Hetz, Katherine; Martini, David; Jordan, Bryan S.; Tracey, Brian H.; Freeman, Jenny E

doi:10.1097/01.ta.0000217357.10617.3d

Cited by 78 publications

(55 citation statements)

References 34 publications

(44 reference statements)

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“…Medical hyperspectral imaging is a novel noninvasive technique that utilizes visible light for the spectral analyses of tissue oxyhemoglobin and deoxyhemoglobin and calculates the oxygen saturation based on their relative abundance (6,30,56). Hyperspectral imaging was performed using OxyVu-2 system (Hypermed, Burlington, MA) according to the manufacturer's instructions.…”

Section: Hyperspectral Imagingmentioning

confidence: 99%

Characterization of a preclinical model of chronic ischemic wound

Roy

Biswas

Khanna

et al. 2009

Physiological Genomics

111

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Chronic ischemic wounds presenting at wound clinics are heterogeneous with respect to etiology, age of the wound, and other factors complicating wound healing. In addition, there are ethical challenges associated with collecting repeated biopsies from a patient to develop an understanding of the temporal dynamics of the mechanisms underlying chronic wounds. The need for a preclinical model of ischemic wound is therefore compelling. The porcine model is widely accepted as an excellent preclinical model for human wounds. A full-thickness bipedicle flap approach was adopted to cause skin ischemia. Closure of excisional wounds placed on ischemic tissue was severely impaired resulting in chronic wounds. Histologically, ischemic wounds suffered from impaired re-epithelialization, delayed macrophage recruitment and poorer endothelial cell abundance and organization. Compared with the pair-matched nonischemic wound, unique aspects of the ischemic wound biology were examined on days 3, 7, 14, and 28 by systematic screening of the wound tissue transcriptome using high-density porcine GeneChips. Ischemia markedly potentiated the expression of arginase-1, a cytosolic enzyme that metabolizes the precursor of nitric oxide l-arginine. Ischemia also induced the SOD2 in the wound tissue perhaps as survival response of the challenged tissue. Human chronic wounds also demonstrated elevated expression of SOD2 and arginase-1. This study provides a thorough database that may serve as a valuable reference tool to develop novel hypotheses aiming to elucidate the biology of ischemic chronic wounds in a preclinical setting.

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Section: Hyperspectral Imagingmentioning

confidence: 99%

Characterization of a preclinical model of chronic ischemic wound

Roy

Biswas

Khanna

et al. 2009

Physiological Genomics

111

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“…There has been widespread application of spectral imaging systems in applications ranging from cytogenetics and 1-4 pathology 5 to oncology. 6,7 The use of spectral imaging to perform blood oximetry, exploiting the different spectral characteristics of oxygenated (HbO 2 ) vs deoxygenated haemoglobin (Hb) has been described in a variety of clinical applications, such as assessing tissue perfusion, [8][9][10] microvascular disease in diabetes, 11 and sickle cell anaemia. 12 …”

Section: Introductionmentioning

confidence: 99%

Spectral imaging of the retina

Mordant

Alabboud

Muyo

et al. 2011

Eye

107

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Introduction The work described here involved the use of a modified fundus camera to obtain sequential hyperspectral images of the retina in 14 normal volunteers and in 1 illustrative patient with a retinal vascular occlusion. Methods The paper describes analysis techniques, which allow oximetry within retinal vessels; these results are presented as retinal oximetry maps. Results Using spectral images, with wavelengths between 556 and 650 nm, the mean oxygen saturation (OS) value in temporal retinal arterioles in normal volunteers was 104.3 ( ± 16.7), and in normal temporal retinal venules was 34.8 (±17.8). These values are comparable to those quoted in the literature, although, the venular saturations are slightly lower than those values found by other authors; explanations are offered for these differences. Discussion The described imaging and analysis techniques produce a clinically useful map of retinal oximetric values. The results from normal volunteers and from one illustrative patient are presented. Further developments, including the recent development of a 'snapshot' spectral camera, promises enhanced non-invasive retinal vessel oximetry mapping.

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“…6,7 Additionally, HSI has been used in murine and porcine models to study cutaneous perfusion during mechanical stress and hemorrhagic shock, respectively. 8,9 HSI is a method of wide-field diffuse reflectance spectroscopy that utilizes a spectral separator to vary the wavelength of light entering a digital camera and provides a diffuse reflectance spectrum for every pixel. These spectra are then compared to standard transmission solutions to calculate the concentration of oxyhemoglobin (Hb-oxy) and deoxyhemoglobin (Hb-deoxy) in each pixel, from which spatial maps of tissue oxygenation are constructed as previously described by Yudovsky et al 10 OxyVu TM -2 (HyperMed TM , Inc. Greenwich, Connecticut) is a commercially available device that generates tissue oxygenation maps of the subpapillary plexus.…”

Section: Introductionmentioning

confidence: 99%

Hyperspectral imaging for early detection of oxygenation and perfusion changes in irradiated skin

Chin

2012

J. Biomed. Opt

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Abstract. Studies examining acute oxygenation and perfusion changes in irradiated skin are limited. Hyperspectral imaging (HSI), a method of wide-field, diffuse reflectance spectroscopy, provides noninvasive, quantified measurements of cutaneous oxygenation and perfusion. This study examines whether HSI can assess acute changes in oxygenation and perfusion following irradiation. Skin on both flanks of nude mice (n ¼ 20) was exposed to 50 Gy of beta radiation from a strontium-90 source. Hyperspectral images were obtained before irradiation and on selected days for three weeks. Skin reaction assessment was performed concurrently with HSI. Desquamative injury formed in all irradiated areas. Skin reactions were first seen on day 7, with peak formation on day 14, and resolution beginning by day 21. HSI demonstrated increased tissue oxygenation on day 1 before cutaneous changes were observed (p < 0.001). Further increases over baseline were seen on day 14, but returned to baseline levels by day 21. For perfusion, similar increases were seen on days 1 and 14. Unlike tissue oxygenation, perfusion was decreased below baseline on day 21 (p < 0.002). HSI allows for complete visualization and quantification of tissue oxygenation and perfusion changes in irradiated skin, and may also allow prediction of acute skin reactions based on early changes seen after irradiation.

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Hyperspectral Imaging: A New Approach to the Diagnosis of Hemorrhagic Shock

Abstract: HSI is a promising noninvasive and noncontact tool for quantifying changes in skin oxygenation during HEM and resuscitation.

Cited by 78 publications

References 34 publications

Characterization of a preclinical model of chronic ischemic wound

Characterization of a preclinical model of chronic ischemic wound

Spectral imaging of the retina

Hyperspectral imaging for early detection of oxygenation and perfusion changes in irradiated skin

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