Hyperspectral Holography and Laser Diffractometry of Erythrocytes

Abstract

Geometrical 3D models of human red blood cells in dry smear were obtained experimentally using hyperspectral holography technique. Corresponding diffraction patterns in the far field diffraction zone were calculated. Visibility values of the diffraction patterns were obtained in cases of low and high red cell distributions width (RDW). Our study reveals that although the visibility is influenced by the cells shapes, this parameter can still be used to assess red cell distribution width in clinical practice.