Hyperspectral evaluation of peritoneal fibrosis in mouse models

Stergar, Jošt; Dolenec, R.; Kojc, Nika; Lakota, Katja; Perše, Martina; Tomšič, Matija; Milanič, Matija

doi:10.1364/boe.387837

Cited by 8 publications

(7 citation statements)

References 28 publications

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“…Results reflect changes in the size and shape of scatters. This reflects structural changes due to the fibrotic cross-linking material, edema, or infiltration of the tissue with immune cells [ 19 ].…”

Section: Resultsmentioning

confidence: 99%

“…using the light characteristics of penetration, absorption, reflection, transmission, and scattering (i.e., scattering parameters). It enables the evaluation of surface alterations as well as the degree of inflammation or fibrosis in the tissue at a depth of 1 mm [ 19 ].…”

Section: Discussionmentioning

confidence: 99%

“…A custom-developed hyperspectral imaging setup [ 19 , 20 , 21 , 22 ], able to operate in both transmission and reflectance geometries, was used to acquire spectral and sample thickness data. In the case of the 1-week experiment, samples were imaged on a black foamed PVC substrate (Forex, Airex AG, Sins, Switzerland) and illuminated from above, recording reflectance data.…”

Section: Methodsmentioning

confidence: 99%

“…In the case of the 3-week experiment, the sample was imaged on a sheet of 3 mm thick opaque white plexiglass (Acrytech, Ljubljana, Slovenia) and was illuminated from the bottom using a halogen light source (Osram, Germany), thus obtaining transmittance data. From the spectra, hemoglobin species volume fractions were extracted using the Beer-Lambert law [ 23 , 24 ] (3 weeks) and custom Kubelka–Munk based model [ 19 ] (1 week), employing reference absorption spectra [ 25 ] and accounting for the sample thickness. To approximate light scattering, power law [ 26 ] was used in both cases.…”

Section: Methodsmentioning

confidence: 99%

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Molecular and Cellular Markers in Chlorhexidine-Induced Peritoneal Fibrosis in Mice

et al. 2022

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Understanding the tissue changes and molecular mechanisms of preclinical models is essential for creating an optimal experimental design for credible translation into clinics. In our study, a chlorhexidine (CHX)-induced mouse model of peritoneal fibrosis was used to analyze histological and molecular/cellular alterations induced by 1 and 3 weeks of intraperitoneal CHX application. CHX treatment for 1 week already caused injury, degradation, and loss of mesothelial cells, resulting in local inflammation, with the most severe structural changes occurring in the peritoneum around the ventral parts of the abdominal wall. The local inflammatory response in the abdominal wall showed no prominent differences between 1 and 3 weeks. We observed an increase in polymorphonuclear cells in the blood but no evidence of systemic inflammation as measured by serum levels of serum amyloid A and interleukin-6. CHX-induced fibrosis in the abdominal wall was more pronounced after 3 weeks, but the gene expression of fibrotic markers did not change over time. Complement system molecules were strongly expressed in the abdominal wall of CHX-treated mice. To conclude, both histological and molecular changes were already present in week 1, allowing examination at the onset of fibrosis. This is crucial information for refining further experiments and limiting the amount of unnecessary animal suffering.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Molecular and Cellular Markers in Chlorhexidine-Induced Peritoneal Fibrosis in Mice

et al. 2022

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“…Abdominal wall histology samples were obtained from a preclinical study of peritonitis 6,7 . In an experimental group of 10 subjects, 3 belonged to the control group (PBS), while the remaining 7 subjects together formed the pure model (3 subjects, CHX) and the treated model (4 subjects, CHX+RVD) groups.…”

Section: Methodsmentioning

confidence: 99%

Fourier-space evaluation of tissue order in animal models of peritonitis

Stergar,

Lakota,

Perše

et al. 2023

Diffuse Optical Spectroscopy and Imaging IX

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In this contribution we show a novel application of Fourier space analysis to determine overall order of tissue architecture in samples of murine abdominal walls from an animal study of induced peritonitis. Such metrices present a necessary steppingstone in the development of digital histology, facilitating both longitudinal as well as large screening studies through introduction of quantitative and rapidly computable biomarkers. The samples were collected from a study including three different experimental groups receiving different intraperitoneal injections; a control group, which received a phosphate-buffered saline solution, a pure model group, receiving chlorhexidine gluconate to induce peritonitis, and a treated model group, which was additionally administered resolvin D1. We present an approach for analysis of hematoxylin and eosin stained histological slices from this study. First, hyperspectral microscopy images of the slides are acquired, and Beer-Lambert law is used to calculate relative volume fraction maps of both stains. Following this decomposition, a Fourier space representation of the eosin cellular image is calculated. Differences between healthy and diseased subjects, which are attributed to necrosis and edema, are then quantified in the Fourier space through the ellipticity of spatial frequency distribution. The proposed metric is shown to significantly discriminate between the healthy and diseased subject (p=0.02) and is additionally strongly linearly correlated to scattering parameter b on macroscopic scale observed in our previous analysis employing macroscopic imaging of whole abdominal walls (linear correlation coefficient of -0.9).

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