Hypersensitive reaction of plum (Prunus domestica) in response to Plum pox virus infection: Changes in gene expression and identification of potential molecular markers

Markiewicz, Monika; Michalczuk, Lech; Neumüller, M.

doi:10.1016/j.scienta.2018.12.034

Cited by 2 publications

(2 citation statements)

References 39 publications

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“…In another study, Markiewicz et al [ 34 ] identified 27 genes implicated in HR against PPV infection in the “Jojo” variety. These genes were selected by comparing hypersensitive and susceptible varieties in healthy and PPV-infected conditions.…”

Section: How Does the Plum Pox Virus Affect The Resistant Prunus Leaves?mentioning

confidence: 99%

Deciphering Prunus Responses to PPV Infection: A Way toward the Use of Metabolomics Approach for the Diagnostic of Sharka Disease

et al. 2021

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Sharka disease, caused by Plum pox virus (PPV), induces several changes in Prunus. In leaf tissues, the infection may cause oxidative stress and disrupt the photosynthetic process. Moreover, several defense responses can be activated after PPV infection and have been detected at the phytohormonal, transcriptomic, proteomic, and even translatome levels. As proposed in this review, some responses may be systemic and earlier to the onset of symptoms. Nevertheless, these changes are highly dependent among species, variety, sensitivity, and tissue type. In the case of fruit tissues, PPV infection can modify the ripening process, induced by an alteration of the primary metabolism, including sugars and organic acids, and secondary metabolism, including phenolic compounds. Interestingly, metabolomics is an emerging tool to better understand Prunus–PPV interactions mainly in primary and secondary metabolisms. Moreover, through untargeted metabolomics analyses, specific and early candidate biomarkers of PPV infection can be detected. Nevertheless, these candidate biomarkers need to be validated before being selected for a diagnostic or prognosis by targeted analyses. The development of a new method for early detection of PPV-infected trees would be crucial for better management of the outbreak, especially since there is no curative treatment.

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Section: How Does the Plum Pox Virus Affect The Resistant Prunus Leaves?mentioning

confidence: 99%

Deciphering Prunus Responses to PPV Infection: A Way toward the Use of Metabolomics Approach for the Diagnostic of Sharka Disease

et al. 2021

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“…To evaluate the genetic background of phenotypic alterations between apple ‘Free Redstar’ neotetraploids and their diploid counterparts, we determined the presence of various Rvi genes using cDNA-AFLP (amplified fragment length polymorphism) analysis, as well as examined the differential expression of Rvi6 gene and other resistance-related genes, such as PR1 (pathogenesis-related protein 1), PR2 (β-1,3-glucanase), WRKY29 (WRKY transcription factor 29), CDPK (calcium-dependent protein kinase), and MPK4 (a mitogen-activated protein kinases). Previously, we successfully used cDNA-AFLP analysis to identify the resistance-related genes in Malus × domestica and Prunus domestica under infection by Erwinia amylovora and the Plum pox virus , respectively [ 29 , 30 ].…”

Section: Introductionmentioning

confidence: 99%

Apple Autotetraploids with Enhanced Resistance to Apple Scab (Venturia inaequalis) Due to Genome Duplication-Phenotypic and Genetic Evaluation

Podwyszyńska

Markiewicz

Broniarek-Niemiec

et al. 2021

IJMS

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Among the fungal diseases of apple trees, serious yield losses are due to an apple scab caused by Venturia inaequalis. Protection against this disease is based mainly on chemical treatments, which are currently very limited. Therefore, it is extremely important to introduce cultivars with reduced susceptibility to this pathogen. One of the important sources of variability for breeding is the process of polyploidization. Newly obtained polyploids may acquire new features, including increased resistance to diseases. In our earlier studies, numerous tetraploids have been obtained for several apple cultivars with ‘Free Redstar’ tetraploids manifesting enhanced resistance to apple scab. In the present study, tetraploids of ‘Free Redstar’ were assessed in terms of phenotype and genotype with particular emphasis on the genetic background of their increased resistance to apple scab. Compared to diploid plants, tetraploids (own-rooted plants) were characterized with poor growth, especially during first growing season. They had considerably shorter shoots, fewer branches, smaller stem diameter, and reshaped leaves. In contrast to own-rooted plants, in M9-grafted three-year old trees, no significant differences between diplo- and tetraploids were observed, either in morphological or physiological parameters, with the exceptions of the increased leaf thickness and chlorophyll content recorded in tetraploids. Significant differences between sibling tetraploid clones were recorded, particularly in leaf shape and some physiological parameters. The amplified fragment length polymorphism (AFLP) analysis confirmed genetic polymorphism of tetraploid clones. Methylation-sensitive amplification polymorphism (MSAP) analysis showed that the level of DNA methylation was twice as high in young tetraploid plants as in a diploid donor tree, which may explain the weaker vigour of neotetraploids in the early period of their growth in the juvenile phase. Molecular analysis showed that ‘Free Redstar’ cultivar and their tetraploids bear six Rvi genes (Rvi5, Rvi6, Rvi8, Rvi11, Rvi14 and Rvi17). Transcriptome analysis confirmed enhanced resistance to apple scab of ‘Free Redstar’ tetraploids since the expression levels of genes related to resistance were strongly enhanced in tetraploids compared to their diploid counterparts.

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Hypersensitive reaction of plum (Prunus domestica) in response to Plum pox virus infection: Changes in gene expression and identification of potential molecular markers

Cited by 2 publications

References 39 publications

Deciphering Prunus Responses to PPV Infection: A Way toward the Use of Metabolomics Approach for the Diagnostic of Sharka Disease

Deciphering Prunus Responses to PPV Infection: A Way toward the Use of Metabolomics Approach for the Diagnostic of Sharka Disease

Apple Autotetraploids with Enhanced Resistance to Apple Scab (Venturia inaequalis) Due to Genome Duplication-Phenotypic and Genetic Evaluation

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