Hyperproduction of 3-hydroxypropionate by Halomonas bluephagenesis

Jiang, Xiao-Ran; Yan, Xu; Yu, Linping; Liu, Xin-Yi; Chen, Guo‐Qiang

doi:10.1038/s41467-021-21632-3

Cited by 60 publications

(33 citation statements)

References 71 publications

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“…It is important to note that the pilot-scale P34HB production by engineered H. bluephagenesis has demonstrated its success as an industrial chassis for NGIB [6]. In addition, recombinant H. bluephagenesis has displayed proven ability for productions of bio-surfactant and bio-emulsifier (PHA surface binding proteins PhaR and PhaP) [23,57], ectoine [42], l-threonine [54], 5-minolevulinic acid (ALA) [53], 3-hydroxypropoinate (3HP) [87] and many more to come, all under open unsterile conditions (Figure 2).…”

Section: Bioproductions By Recombinant Halomonas Sppmentioning

confidence: 99%

Halomonasas a chassis

Chen

2021

Essays in Biochemistry

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With the rapid development of systems and synthetic biology, the non-model bacteria, Halomonas spp., have been developed recently to become a cost-competitive platform for producing a variety of products including polyesters, chemicals and proteins owing to their contamination resistance and ability of high cell density growth at alkaline pH and high salt concentration. These salt-loving microbes can partially solve the challenges of current industrial biotechnology (CIB) which requires high energy-consuming sterilization to prevent contamination as CIB is based on traditional chassis, typically, Escherichia coli, Bacillus subtilis, Pseudomonas putida and Corynebacterium glutamicum. The advantages and current status of Halomonas spp. including their molecular biology and metabolic engineering approaches as well as their applications are reviewed here. Moreover, a systematic strain engineering streamline, including product-based host development, genetic parts mining, static and dynamic optimization of modularized pathways and bioprocess-inspired cell engineering are summarized. All of these developments result in the term called next-generation industrial biotechnology (NGIB). Increasing efforts are made to develop their versatile cell factories powered by synthetic biology to demonstrate a new biomanufacturing strategy under open and continuous processes with significant cost-reduction on process complexity, energy, substrates and fresh water consumption.

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Section: Bioproductions By Recombinant Halomonas Sppmentioning

confidence: 99%

Halomonasas a chassis

Chen

2021

Essays in Biochemistry

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“…An alternative for the latter is the use of robust extremophilic strains able to produce the target compounds (e.g., PHA) under simpli ed process conditions, in open unsterile, continuous fermentation facilities where most other organisms are unable to proliferate. The extremophiles based process seems to be suitable for simple growth on mixed degradation products, including fatty acids, plastic monomers and food wastes [67][68][69][70]. In mixed cultures and consortia exist in addition to intraspecies interactions, e.g., quorum sensing, interspecies interactions between cells of the different species.…”

Section: Mixed Cultures In Industrial Applicationsmentioning

confidence: 99%

MIXed Plastics Biodegradation and UPcycling Using Microbial Communities: The EU Horizon 2020 Project MIX-UP

Ballerstedt

Tiso

Wierckx

et al. 2021

Preprint

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This article introduces the EU Horizon 2020 research project MIX-UP, “Mixed plastics biodegradation and upcycling using microbial communities”. The project focuses on the ambitious vision to change the traditional linear value chain of plastics to a sustainable, biodegradable based one. In MIX-UP, plastic mixtures containing five of the top six fossil-based recalcitrant plastics (PE, PUR, PP, PET, and PS), along with upcoming biobased and biodegradable plastics (bioplastics) such as PHA and PLA, will be used as feedstock for microbial transformations. The generated new workflow increases recycling quotas and adds value to present poorly recycled plastic waste streams. Consecutive controlled enzymatic and microbial degradation of mechanically pre-treated plastics waste combined with subsequent microbial conversion to polymers and value-added chemicals by mixed cultures. Through optimization of known plastic-degrading enzymes by integrated protein engineering, high specific binding capacities, stability, and catalytic efficacy towards a broad spectrum of plastic polymers under high salt content and temperature conditions will be achieved. Another focus lies in the search and isolation of novel enzymes active on recalcitrant polymers. MIX-UP will also enhance the production of enzymes and formulate enzyme cocktails tailored to specific waste streams. In vivo and in vitro application of these cocktails enables stable, self-sustaining microbiomes to convert the released plastic monomers selectively into value-added products, key building blocks, and biomass. Any of the remaining material recalcitrant to the enzymatic activity will be recirculated into the process by physicochemical treatment. The Chinese-European MIX-UP is a multidisciplinary and industry-participating consortium to address the market need for novel sustainable routes to valorize plastic waste streams. MIX-UP realizes a circular (bio) plastic economy and will contribute where mechanical and chemical plastic recycling show limits.

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“…PHA) under simplified process conditions, in open unsterile, continuous fermentation facilities where most other organisms are unable to proliferate. The extremophiles based process seems to be suitable for simple growth on mixed degradation products, including fatty acids, plastic monomers and food wastes [ 68 – 71 ]. In mixed cultures and consortia exist in addition to intraspecies interactions, e.g.…”

Section: Introductionmentioning

confidence: 99%

MIXed plastics biodegradation and UPcycling using microbial communities: EU Horizon 2020 project MIX-UP started January 2020

et al. 2021

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This article introduces the EU Horizon 2020 research project MIX-UP, "Mixed plastics biodegradation and upcycling using microbial communities". The project focuses on changing the traditional linear value chain of plastics to a sustainable, biodegradable based one. Plastic mixtures contain five of the top six fossil-based recalcitrant plastics [polyethylene (PE), polyurethane (PUR), polypropylene (PP), polyethylene terephthalate (PET), polystyrene (PS)], along with upcoming bioplastics polyhydroxyalkanoate (PHA) and polylactate (PLA) will be used as feedstock for microbial transformations. Consecutive controlled enzymatic and microbial degradation of mechanically pre-treated plastics wastes combined with subsequent microbial conversion to polymers and value-added chemicals by mixed cultures. Known plastic-degrading enzymes will be optimised by integrated protein engineering to achieve high specific binding capacities, stability, and catalytic efficacy towards a broad spectrum of plastic polymers under high salt and temperature conditions. Another focus lies in the search and isolation of novel enzymes active on recalcitrant polymers. MIX-UP will formulate enzyme cocktails tailored to specific waste streams and strives to enhance enzyme production significantly. In vivo and in vitro application of these cocktails enable stable, self-sustaining microbiomes to convert the released plastic monomers selectively into value-added products, key building blocks, and biomass. Any remaining material recalcitrant to the enzymatic activities will be recirculated into the process by physicochemical treatment. The Chinese–European MIX-UP consortium is multidisciplinary and industry-participating to address the market need for novel sustainable routes to valorise plastic waste streams. The project's new workflow realises a circular (bio)plastic economy and adds value to present poorly recycled plastic wastes where mechanical and chemical plastic recycling show limits.

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Hyperproduction of 3-hydroxypropionate by Halomonas bluephagenesis

Cited by 60 publications

References 71 publications

Halomonasas a chassis

Halomonasas a chassis

MIXed Plastics Biodegradation and UPcycling Using Microbial Communities: The EU Horizon 2020 Project MIX-UP

MIXed plastics biodegradation and UPcycling using microbial communities: EU Horizon 2020 project MIX-UP started January 2020

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