Hyperpolarized MAS NMR of unfolded and misfolded proteins

Determining isoleucine side-chain rotamer-sampling in proteins from 13C chemical shift

“…S7 (ESI †). 21 Calculating the peak positions using the chemical shift profiles also readily identifies { g m ,t}, { g m ,g m } and {g p ,t} as the major states. Moreover, the populations of the three major states determined from peak volumes in the DQSQ spectrum agree well with those determined in solution from 13 C chemical shifts, in particular a substantial population of { g p ,t}.…”

Biologische Festkörper-NMR-Spektroskopie in der Strukturbiologie

“…Interessante Anwendungsmöglichkeiten der Festköper-NMR ergeben sich auch mithilfe der dynamischen nuklearen Polarisation (DNP), die zur Signalverstärkung eingesetzt wird. Mittels DNPverstärkter Festköper-NMR-Spektroskopie konnte u. a. experimentell gezeigt werden, dass intrinsisch ungefaltete Proteine bereits im Grundzustand eine Vielzahl unterschiedlicher -teilweise gefalteter -Konformationen durchlaufen [5]. Auch Photointermediate des Rhodopsins konnten kürzlich mittels DNP charakterisiert werden [6].…”

Activation of Cytochrome C Peroxidase Function Through Coordinated Foldon Loop Dynamics upon Interaction with Anionic Lipids

“…In vitro, there are many studies reporting on the interactions of cyt c with non-CL anionic lipids, including PG, showing the potential for similar interactions to those underpinning CL-induced peroxidase activation [28][29][30][31]. Yet, to the best of our knowledge there is no record of a direct involvement of PG in place of CL in the apoptotic pathway in vivo, with studies showing CL to be the preferred substrate for cyt c binding and peroxidation [2,3,32,33] [34]: the blue foldon (N and C-terminal helices, residues 1-14 and 88-104), the green foldon (helix [61][62][63][64][65][66][67][68][69] and Ω loop [20][21][22][23][24][25][26][27][28][29][30][31][32][33][34][35], the gray foldon (Ω loop [40][41][42][43][44][45][46][47][48][49][50][51]…”