Hyperpigmentation Analysis of Tal Enzyme on Danio Rerio Obtained From Basella Alba

Abstract

Tyrosine ammonia lyase (TAL) is an enzyme required for transforming L -Tyrosine into p -Coumaric acid. The objective of the experiment is to extract TAL from Basella alba to treat hyperpigmentation a condition caused due to excessive production of Melanin pigment accumulating in the epidermal cells. Firstly, the separation of proteins was done using an organic solvent, acetone. In the second step the presence of the enzyme was confirmed by the formation of p-coumaric acid which was detected by using UV-Visible spectrophotometer at 410nm. The activity of enzyme was enhanced by certain compounds extracted from Trigonellafoenum-graecum, and compounds were identified by using gc-ms.All the compounds were docked to TAL structure using Autodock software; Eugenol was identified with high affinity. Then the embryos of zebrafish were used to study enzyme activity in vivo condition. TAL enzyme was treated with embryos before the pigmentation on the skin with control where enzyme was not added. TAL treated embryos has not shown pigmentation on skin where control shows the pigmentations.