Hyperphosphorylation of the Group A Streptococcal Control of Virulence Regulator Increases Promoter Occupancy Specifically at Virulence Factor-Encoding Genes

Abstract

Given its key role in pathogenesis of Gram-positive bacteria, CovR is one of the most important members of the OmpR/PhoB family of transcriptional regulators. Herein we extend recent GAS CovR global binding analyses done in emm1 to a non- emm1 strain, which is important considering the known inter- emm -type heterogeneity in GAS CovRS function.

LiaR-dependent gene expression contributes to antimicrobial responses in group AStreptococcus

“…High-throughput sequencing on prepared sample libraries was performed by Genewiz (Azenta Life Sciences) to generate a minimum of 50M 150-bp paired reads per replicate/input sample. Analysis was performed as previously described (Horstmann et al 2023; DebRoy et al 2021) using CLC Genomics Workbench (v 23) Transcription Factor ChIP-seq module.…”

“…Briefly, strains were grown to mid-logarithmic phase (OD600 ~0.4) in 40 mL THY, proteins crosslinked to DNA with 1% formaldehyde, cells harvested by centrifugation, flash-frozen, and stored at -80 o C until processed. Processing of replicate samples for ChIP-seq was performed as previously described (Horstmann et al 2023;DebRoy et al 2021). High-throughput sequencing on prepared sample libraries was performed by Genewiz (Azenta Life Sciences) to generate a minimum of 50M 150-bp paired reads per replicate/input sample.…”