Hyperoside Induces Endogenous Antioxidant System to Alleviate Oxidative Stress

Abstract

Background:Hyperoside, a flavonoid which is mainly found in Hypericum perforatum L., has many biological effects. One of the most important effects is to prevent the oxidative stress induced by reactive oxygen species. However, the molecular mechanisms underlying its effect are not fully understood. Oxidative stress is implicated in the occurrence of various physical diseases. A wide array of enzymatic antioxidant defense systems include NADH: quinone oxidoreductase 1, superoxide dismutase, and heme oxygenase-1 (HO-1). In the present study, the protective effects of hyperoside against hydrogen peroxide-induced oxidative stress in human lens epithelial cells, HLE-B3, were investigated in terms of HO-1 induction.Methods:The protein and mRNA expressions of HO-1 were examined by Western blotting and reverse transcriptase-PCR assays, respectively. To evaluate the ability of hyperoside to activate nuclear factor erythroid 2-related factor 2 (Nrf2), Western blotting and electrophoretic mobility shift assay were performed with nuclear extracts prepared from HLE-B3 cells treated with hyperoside. The activation of extracellular signal-regulated kinase (ERK), the upstream kinase of Nrf2 signaling, was monitored by Western blot analysis. The protective effect of hyperoside in HLE-B3 cells against hydrogen peroxide was performed by MTT assay.Results:Hyperoside increased both the mRNA and protein expression of HO-1 in a time- and dose-dependent manner. In addition, hyperoside elevated the level of of Nrf2 and its antioxidant response element-binding activity, which was modulated by upstream of ERK. Moreover, it activated ERK and restored cell viability which was decreased by hydrogen peroxide.Conclusions:Hyperoside is an effective compound to protect cells against oxidative stress via HO-1 induction.

Hepatoprotective Effect of Cuscuta campestris Yunck. Whole Plant on Carbon Tetrachloride Induced Chronic Liver Injury in Mice

“…Although hyperoside and quercetin have been detected in both of seeds and whole plant of C. campestris , their amounts and the other flavonoids are different [4,20]. Hyperoside have been shown to increase the level of heme oxygenase-1, an important enzyme in antioxidant defense systems to reduce oxidative stress [21]. Quercetin has shown a high antioxidant activity by reducing the production of reactive oxygen species and nitric oxide [20,22].…”

Hyperoside pre-treatment prevents glomerular basement membrane damage in diabetic nephropathy by inhibiting podocyte heparanase expression

“…It can protect cells against oxidative stress and oxidation-related apoptosis 29, 54, 55 . Hyperoside displayed potential anti-inflammatory on high glucose induced inflammation in vitro and in vivo 27, 28 .…”

Phytochemical Composition and Biological Activities of Dyssodia tagetiflora Lag.

“…In this study, we demonstrate that one of the main components of the DTME is hyperoside. Several studies have demonstrated the antioxidant cytoprotective effect of hyperoside on the damage induced by H 2 O 2 in different cell lines, as in lung fibroblast cells, hepatic cells (L02), and human lens epithelial cells (HLE‐B3) . Additionally, several flavonoids have been shown to exhibit cytoprotective activity against cadmium‐induced toxicity .…”

“…[8] Several pharmacological properties of the genus have been studied, including the finding that the chloroformic extract of the aerial parts of D. paposa is active against the fungus Trichophyton mentagrophytes (MIC 1000 lg/mL). This extract was also found to be cytotoxic against cancer cell lines of the colon (HT-29), breast (MCF-7), and lung (A-549) (ED 50 30.25 lg/mL; 46.24 lg/mL; 50.46 lg/mL, respectively), and against Artemia salina (LD 50 40.01 lg/mL). [9] The chloroform/methanol (1:1) extract of D. pinnata caused a decrease in the tone and amplitude of the spontaneous contractions of isolated rat ileum in a concentration-dependent manner, with an IC 50 of 10.88 AE 2.19 lg/mL.…”