Hyperoside Attenuates Bleomycin-Induced Pulmonary Fibrosis Development in Mice

Huang, Jiao; Xiang, Tong; Zhang, Li; Zhang, Yuan; Wang, Lei; Wang, Dongguang; Zhang, Shijie; Fan, Hong

doi:10.3389/fphar.2020.550955

Cited by 37 publications

(14 citation statements)

References 41 publications

(73 reference statements)

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“…Lung tissues were made into paraffin sections (4 µm) and treated with Masson staining [ 18 ]. Collagen fibers, mucus, and cartilage were blue, cytoplasm, muscle, cellulose and glial were red, and cell nuclei were blue-black.…”

Section: Methodsmentioning

confidence: 99%

RETRACTED ARTICLE: Restoring microRNA-499-5p Protects Sepsis-Induced Lung Injury Mice Via Targeting Sox6

Zhang

Yao

et al. 2021

Nanoscale Res Lett

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Background MicroRNAs (miRs) are known to participate in sepsis; hence, we aim to discuss the protective effect of miR-499-5p targeting sex-determining region Y-related high-mobility-group box 6 (Sox6) on sepsis-induced lung injury in mice. Methods The sepsis-induced lung injury model was established by cecal ligation and puncture. The wet/dry weight (W/D) ratio, miR-499-5p, Sox6, Caspase-3 and Caspase-9 expression in lung tissues of mice were tested. Lung injury score, collagen fibers and the degree of pulmonary fibrosis in lung tissues were determined. Further, the cell apoptosis in lung tissues was measured. The inflammatory factors contents and oxidative stress indices in bronchoalveolar lavage fluid (BALF) and lung tissues were detected via loss- and gain-of-function assays. The targeting relation between miR-499-5p and Sox6 was verified. Results W/D ratio and Sox6 were increased while miR-499-5p was decreased in lung tissues of sepsis-induced lung injury mice. Restored miR-499-5p or depleted Sox6 alleviated lung tissues pathology, reduced lung injury score, collagen fibers, the degree of pulmonary fibrosis, TUNEL positive cells, Caspase-3 and Caspase-9 protein expression and inflammatory factors contents in BALF and lung tissues as well as oxidative stress response in lung tissues of sepsis-induced lung injury mice. miR-499-5p targeted Sox6. Conclusion High expression of miR-499-5p can attenuate cell apoptosis in lung tissues and inhibit inflammation of sepsis-induced lung injury mice via depleting Sox6, and it is a potential candidate marker and therapeutic target for sepsis-induced lung injury.

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Section: Methodsmentioning

confidence: 99%

RETRACTED ARTICLE: Restoring microRNA-499-5p Protects Sepsis-Induced Lung Injury Mice Via Targeting Sox6

Zhang

Yao

et al. 2021

Nanoscale Res Lett

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“…However, as mentioned above, the presence of hesperidin and hyperoside in this plant was brought to the literature for the first time with this study. There are some literature data on the contribution of these compounds to antioxidant activity with some other plants or with these compounds themselves (Ku et al, 2014;Hao et al, 2016;Yatao et al, 2018;Gao et al, 2019;He et al, 2019;Kim et al, 2019;Musa et al, 2019;Aggarwal et al, 2020;Huang et al, 2020). These findings support the correlation coefficient data obtained from the present study.…”

Section: Discussionsupporting

confidence: 90%

Phenolic profile, antioxidant and enzyme inhibitory activity of the ethyl acetate, methanol and water extracts of Capparis spinosa L.

Kırkan

Ceylan

Sarıkürkçü

et al. 2021

International Journal of Secondary Metabolite

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In this study, it was aimed to determine the phytochemical compositions and biological activities of ethyl acetate (EtOAc), methanol (MeOH) and water extracts obtained from the aerial parts of Capparis spinosa L. As a result of spectrophotometric analyzes, MeOH extract was found to be richer in terms of both phenolics and flavonoids compared to other extracts [81.45 mg GAEs (gallic acid equivalent)/g and 36.57 mg RE (rutin equivalent)s/g, respectively], while chromatographic analyzes showed that the extract in question contains a significant amount of hepseridin (72927.48 µg/g), quercetin (1335.88 µg/g), hyperoside (1227.73 µg/g), and 4-hydroxybenzoic acid (924.08 µg/g). Phosphomolybdenum, 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2-azinobis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical scavenging, Cupric Reducing Antioxidant Power (CUPRAC) and Ferric Reducing Antioxidant Power (FRAP) reducing and ferrous ion chelating activity tests resulted in superiority of MeOH extract [371.0, 44.93, 56.46, 91.77, 52.61 mg TEs (trolox equivalent)/g and 14.85 mg EDTAEs/g, respectively]. On the other hand, EtOAc extract exhibited higher activity than other extracts in acetylcholinesterase (AChE), butyrylcholinesterase (BChE), α-amylase, and α-glucosidase inhibitory activity tests [3.29, 2.12 mg GALAEs (galanthamine equivalent)/g, 541.01 and 1584.20 mg ACEs (acarbose equivalent)/g, respectively]. The tyrosinase inhibitory activity test resulted in the superiority of MeOH extract [41.90 mg KAEs (kojic acid equivalent)/g]. A strong correlation was determined between the phenolic and flavonoid contents of the extracts and their antioxidant activities.

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“…As a flavonoid, hyperoside has many physiological properties, such as anti-inflammatory, antispasmodic, diuretic, and antitussive properties, and can lower blood pressure, lower cholesterol, and protect the heart and cerebral blood vessels; thus, it is an important natural product. The development of new varieties of okra with increased hyperoside content has an important impact on the medical and economic benefits of the plant 54 – 56 . This research lays a molecular foundation for the development of fine varieties of okra and provides a new research direction related to flower development in other non-model plants.…”

Section: Discussionmentioning

confidence: 99%

Hyperoside promotes pollen tube growth by regulating the depolymerization effect of actin-depolymerizing factor 1 on microfilaments in okra

et al. 2021

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Mature pollen germinates rapidly on the stigma, extending its pollen tube to deliver sperm cells to the ovule for fertilization. The success of this process is an important factor that limits output. The flavonoid content increased significantly during pollen germination and pollen tube growth, which suggests it may play an important role in these processes. However, the specific mechanism of this involvement has been little researched. Our previous research found that hyperoside can prolong the flowering period of Abelmoschus esculentus (okra), but its specific mechanism is still unclear. Therefore, in this study, we focused on the effect of hyperoside in regulating the actin-depolymerizing factor (ADF), which further affects the germination and growth of pollen. We found that hyperoside can prolong the effective pollination period of okra by 2–3-fold and promote the growth of pollen tubes in the style. Then, we used Nicotiana benthamiana cells as a research system and found that hyperoside accelerates the depolymerization of intercellular microfilaments. Hyperoside can promote pollen germination and pollen tube elongation in vitro. Moreover, AeADF1 was identified out of all AeADF genes as being highly expressed in pollen tubes in response to hyperoside. In addition, hyperoside promoted AeADF1-mediated microfilament dissipation according to microfilament severing experiments in vitro. In the pollen tube, the gene expression of AeADF1 was reduced to 1/5 by oligonucleotide transfection. The decrease in the expression level of AeADF1 partially reduced the promoting effect of hyperoside on pollen germination and pollen tube growth. This research provides new research directions for flavonoids in reproductive development.

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Hyperoside Attenuates Bleomycin-Induced Pulmonary Fibrosis Development in Mice

Cited by 37 publications

References 41 publications

RETRACTED ARTICLE: Restoring microRNA-499-5p Protects Sepsis-Induced Lung Injury Mice Via Targeting Sox6

RETRACTED ARTICLE: Restoring microRNA-499-5p Protects Sepsis-Induced Lung Injury Mice Via Targeting Sox6

Phenolic profile, antioxidant and enzyme inhibitory activity of the ethyl acetate, methanol and water extracts of Capparis spinosa L.

Hyperoside promotes pollen tube growth by regulating the depolymerization effect of actin-depolymerizing factor 1 on microfilaments in okra

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