Hypermethylation of Death-Associated Protein Kinase ( DAPK1 ) and its association with oral carcinogenesis - An experimental and meta-analysis study

Jayaprakash, Chinchu; Varghese, Vinay Koshy; Bellampalli, Ravishankara; Radhakrishnan, Raghu; Ray, Sujoy; Kabekkodu, Shama Prasada; Satyamoorthy, Kapaettu

doi:10.1016/j.archoralbio.2017.03.024

Cited by 9 publications

(2 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A meta-analysis study revealed that the DNA hypermethylation of the Dapk1 gene promoter was a promising prognostic and diagnostic biomarker [21,40]. Dapk and MGMT show potential as epigenetic markers in HPV-negative oral and oropharyngeal squamous cell carcinoma [11].…”

Section: Discussionmentioning

confidence: 99%

Epigenetic methylation changes: implication as biomarkers in oral and maxillofacial area cancers

Aghiorghiesei

Irimie

Braicu

et al. 2023

Medicine and Pharmacy Reports

View full text Add to dashboard Cite

Background/Aim. Squamous cell carcinoma (SCC) is the most frequent cancer of the head and neck area in the oral cavity. Epigenetic alterations in oral and maxillofacial area cancers are urgently needed to be investigated, as the observed changes might have crucial diagnostic value for personalized medicine. Methods. Our study aimed to identify the most frequently hypermethylated tumor suppressor gene promoters in OSCC, followed by correlation analysis with the patients’ survival. We evaluated the methylation status of the promoters in a panel of 22 tumor suppressor genes in Romanian (n=9) and Bulgarian (n=12) patient groups suffering from oral and maxillofacial area cancers. The extracted DNA was further digested through EpiTect Methyl II PCR Array System containing methylation-sensitive and methylation-dependent restriction enzymes, followed by specific amplification of the products obtained by qPCR and data analysis using the online platform provided by the producer. Results. Different methylation patterns were observed in the tumor suppressor genes’ promoters. Among them, the methylation profile of Cccnd2, Chd1, Cdh13, Cdkn1c, Neurog1, Gstp1, and Runx3 genes further correlated with overall survival rates. Conclusions. Our data emphasize that epigenetic alterations are responsible for the clinical heterogeneity of oral and maxillofacial area cancers and significantly impact on patient survival. Additional investigation on a larger patient cohort should validate these potential biomarkers.

show abstract

Section: Discussionmentioning

confidence: 99%

Epigenetic methylation changes: implication as biomarkers in oral and maxillofacial area cancers

Aghiorghiesei

Irimie

Braicu

et al. 2023

Medicine and Pharmacy Reports

View full text Add to dashboard Cite

show abstract

“… 51 Without effective early diagnosis biomarkers and therapeutic strategies, the prognosis of EC is relatively poor, with a 5-year survival rate of <20%, although it may be variable in different histotypes. 52 Aberrant DNA methylation in the TSG promoter has been identified as an important mechanism of tumorigenesis and progression of several cancers, including cervical cancer, 10 oral cancer, 53 and colorectal cancer. 54 In addition, as a relatively early molecular change, aberrant methylation was reported to be a potential biomarker for early cancer diagnosis.…”

Section: Discussionmentioning

confidence: 99%

Role of <em>MLH1</em> methylation in esophageal cancer carcinogenesis and its clinical significance

Ye²,

Wang

et al. 2018

OTT

View full text Add to dashboard Cite

Abstract:The mutL homolog-1 (MLH1) is a DNA mismatch repair gene and has been reported to be frequently methylated in numerous cancers. However, the association between MLH1 methylation and esophageal cancer (EC), as well as its clinical significance, remains unclear. Hence, we conducted a systematic meta-analysis based on 19 articles (including 1384 ECs, 345 premalignant lesions, and 1244 healthy controls). Our analysis revealed that the frequency of MLH1 methylation was significantly elevated during EC carcinogenesis. In addition, we observed that MLH1 promoter methylation was associated with age (odds ratio [OR]=1.79; 95% CI =1.20-2.66), advanced tumor grade (OR=3.7; 95% CI =2.37-5.77), lymph node metastasis (OR=2.65; 95% CI =1.81-3.88), distant metastasis (OR=7.60; 95% CI =1.23-47.19), advanced clinical stage (OR=4.46; 95% CI =2.88-6.91), and poor prognosis in EC patients (hazard ratio =1.64, 95% CI =1.00-2.69). The pooled sensitivity, specificity, and area under the curve of MLH1 methylation in EC patients versus healthy individuals were 0.15, 0.99, and 0.77, respectively. Our findings indicate that MLH1 methylation is involved in the carcinogenesis, progression, and metastasis of EC. Moreover, methylated MLH1 could be a potential diagnostic and prognostic biomarker for EC.

show abstract

DNA methylation in the genesis, progress and prognosis of head and neck cancer

Guo

Liu

et al. 2023

Holist Integ Oncol

View full text Add to dashboard Cite

Epigenetic alteration studies in cancer research have been progressing rapidly in recent years. DNA methylation, including DNA hypermethylation and DNA hypomethylation, is one of the main epigenetic alterations in head and neck cancer development. Here, we review recent advances in DNA methylation and factors affecting DNA methylation, including DNA methylation enzymes, HPV status and smoking and drinking habits, in the field of head and neck cancer occurrence, progression, metastasis, and prognosis, hoping to shed light on how DNA methylation interacts with head and neck cancer and lay a foundation for future prognosis prediction and therapy.

show abstract

Hypermethylation of Death-Associated Protein Kinase ( DAPK1 ) and its association with oral carcinogenesis - An experimental and meta-analysis study

Cited by 9 publications

References 54 publications

Epigenetic methylation changes: implication as biomarkers in oral and maxillofacial area cancers

Epigenetic methylation changes: implication as biomarkers in oral and maxillofacial area cancers

Role of <em>MLH1</em> methylation in esophageal cancer carcinogenesis and its clinical significance

DNA methylation in the genesis, progress and prognosis of head and neck cancer

Contact Info

Product

Resources

About