Hypermethylation of CpGs in the Promoter of the COL1A1 Gene in the Aged Periodontal Ligament

Ohi, Takashi; Uehara, Yoshio; Tomita, Masaki; Watanabe, Makoto; Ono, Tetsuya

doi:10.1177/154405910608500308

Cited by 32 publications

(28 citation statements)

References 25 publications

(37 reference statements)

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“…Ohi et al [34] demonstrated that suppression of collagen expression in aged PDLC may be related to DNA hypermethylation of the regulatory region of the type I collagen gene, a mechanism that inhibits gene expression during the aging process of several genes.…”

Section: Discussionmentioning

confidence: 98%

Influence of Aging on Biological Properties of Periodontal Ligament Cells

Benatti¹,

Silvério

Casati

et al. 2008

Connective Tissue Research

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The majority of patients eligible for periodontal regenerative therapies are aged subjects. Since periodontal ligament cells (PDLC) are essential for periodontal regeneration, the aim of the present study was to determine the effect of cellular aging on PDLC, including genes associated with extracellular matrix metabolism and growth-associated factors. PDLC cultures were obtained from subjects aged 15 to 20 years and subjects aged more than 60 years. Proliferation, cell viability, mineralization assays, and mRNA levels were assessed for type I and III collagen, platelet-derived growth factor (PDGF)-1, basic fibroblast growth factor (bFGF), metalloproteinase (MMP)-2 and-8, and tissue inhibitor of metalloproteinases (TIMP)-1 and-2. Data analysis demonstrated that aging negatively influenced cell proliferation and mineral nodule formation (p < 0.05). Gene expression analysis further showed that mRNA levels for bFGF, PDGF-1, and TIMP-2 were not affected by aging (p > 0.05). In addition, mRNA levels for type I and III collagen were significantly lower in aged cells (p < 0.05), whereas MMP-2 and-8 and TIMP-1 mRNA levels were higher (p < 0.05). Within the limits of the present study, data analysis suggests that aging modulates important biological properties of periodontal ligament cells, diminishes the potential for mineral nodule formation, and favors extracellular matrix degradation.

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Section: Discussionmentioning

confidence: 98%

Influence of Aging on Biological Properties of Periodontal Ligament Cells

Benatti¹,

Silvério

Casati

et al. 2008

Connective Tissue Research

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“…This is the most abundant protein in the periodontium and maintains the attachment of teeth with the alveolar bone and soft tissues (Bartold et al 2000). It has been observed that type I collagen decreases in the periodontium with increasing age (Ohi et al 2006, Lim et al 2014). This phenomenon was recently reproduced by a study that identified a progressive atrophy of the tooth supporting structures associated with ageing in rats (Leong et al 2012).…”

Section: The Complex Relationship Between Periodontal Tissue Destructmentioning

confidence: 99%

Ageing, dental caries and periodontal diseases

López

Göstemeyer

et al. 2017

J Clinic Periodontology

210

138

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Aim: To review the burden of caries and periodontitis in the elderly, changes with age that can explain this burden, and the vulnerability to disease of elderly populations. Methods: An assessment of surveys in two populations was conducted. Indicators for caries were identified by updating a systematic review. Secular trends for smoking and type 2 diabetes were discussed. Results: Changes in the susceptibility to periodontitis with age may be explained by exposure to pro-inflammatory conditions and changes in the healing capacity of cells and tissues. Due to accumulated periodontal destruction, the number of surfaces at risk for caries increases. The sequels of restorative treatment contribute to an increased susceptibility for caries development. Population-based surveys in the United States and Germany demonstrate a high caries experience among elderly people. A comparison of surveys demonstrates a relative improvement of periodontal health among elderly during the last few decades. Nevertheless, prevalence estimates for periodontitis remain high. Risk indicators for root caries include caries experience, the number of surfaces at risk and poor oral hygiene. Secular trends of main risk factors for periodontitis and their likely influence on the future periodontitis burden in the elderly are discussed. Conclusion: Caries and periodontitis burden in the elderly remain high.

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“…It is reasonable to assume that environmental factors, such as exercise, may also work through epigenetic mechanisms to modulate the risk of musculoskeletal soft tissue injuries 9. In support of this, an age-dependent increase in the hypermethylation of the COL1A1 promoter in periodontal ligaments has been reported 60. Future work should therefore consider epigenomic techniques, also within the tissue of interest, such as methylation sequencing and ChIP sequencing, which can be used to identify protein-DNA and protein-RNA interactions.…”

Section: Future Workmentioning

confidence: 94%

Biological variation in musculoskeletal injuries: current knowledge, future research and practical implications

2015

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Evidence from familial and genetic association studies have reported that DNA sequence variants play an important role, together with non-genetic factors, in the aetiology of both exercise-associated and occupational-associated acute and chronic musculoskeletal soft tissue injuries. The associated variants, which have been identified to date, may contribute to the interindividual variation in the structure and, by implication, mechanical properties of the collagen fibril and surrounding matrix within musculoskeletal soft tissues, as well as their response to mechanical loading and other stimuli. Future work should focus on the establishment of multidisciplinary international consortia for the identification of biologically relevant variants involved in modulating injury risk. These consortia will improve the limitations of the published hypothesis-driven genetic association studies, since they will allow resources to be pooled in recruiting large well-characterised cohorts required for whole-genome screening. Finally, clinicians and coaches need to be aware that many direct-to-consumer companies are currently marketing genetic tests directly to athletes without it being requested by an appropriately qualified healthcare professional, and without interpretation alongside other clinical indicators or lifestyle factors. These specific genetic tests are premature and are not necessarily required to evaluate susceptibility to musculoskeletal soft tissue injury. Current practice should rather consider susceptibility through known risk factors such as a positive family history of a specific injury, a history of other tendon and/or ligament injuries and participation in activities associated with the specific musculoskeletal injuries. Potential susceptible athletes may then be individually managed to reduce their risk profile.

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Hypermethylation of CpGs in the Promoter of the COL1A1 Gene in the Aged Periodontal Ligament

Cited by 32 publications

References 25 publications

Influence of Aging on Biological Properties of Periodontal Ligament Cells

Influence of Aging on Biological Properties of Periodontal Ligament Cells

Ageing, dental caries and periodontal diseases

Biological variation in musculoskeletal injuries: current knowledge, future research and practical implications

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