Hypermethylation and reduced expression of Gtl2, Rian and Mirg at the Dlk1-Dio3 imprinted locus as a marker for poor developmental potential of mouse embryonic stem cells

Schacker, Maria; Cheng, Ying; Eckersley-Maslin, Mélanie; Snaith, Richard Michael; Colledge, William H.

doi:10.1016/j.scr.2020.101931

Cited by 4 publications

(1 citation statement)

References 42 publications

(52 reference statements)

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“…Firstly, Takeshi Saito et al found that a tandem repeat array in IG-DMR was essential for imprinting of paternal allele at the DLK1-Dio3 domain during embryonic development [18]. And, Maria Schacker et al reported that hypermethylation and down-expression of Gtl2, Rian and Mirg at the DLK1-Dio3 imprinted locus were positive correlations of poor developmental potential of mouse embryonic stem cells [19]. Chu-Fan Mo et al indicated that loss of non-coding RNA expression from the DLK1-Dio3 imprinted locus correlates with reduced neural differentiation potential in human embryonic stem cell lines [20].…”

Section: Introductionmentioning

confidence: 99%

miR-31-5p from placental and peripheral blood exosomes is a potential biomarker to diagnose preeclampsia

Zou

Geng

et al. 2022

Hereditas

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Background Preeclampsia, a multisystem disorder of unknown etiology, is one of the leading causes of maternal and perinatal morbidity and mortality. Identifying sensitive, noninvasive markers can aid its prevention and improve prognosis. microRNAs (miRs), which function as negative regulators of gene expression, are closely related to preeclampsia occurrence and development. Herein we investigated the relationship between the DLK1-Dio3 imprinted miR cluster derived from placental and peripheral blood exosomes of pregnant women with preeclampsia and routine clinical diagnostic indicators, and also determined its potential as a noninvasive diagnostic marker. Methods Exosomes were extracted from the placenta and peripheral blood of pregnant women with preeclampsia. Results qPCR data indicated that the expression level of miRs, such as miR-134, miR-31-5p, miR-655, miR-412, miR-539, miR-409, and miR-496, in pregnant women with preeclampsia was significantly lower than that in healthy controls; miR-31-5p expression was the most different. Gene ontology analysis predicted that genes negatively regulated by miR-31-5p were mainly enriched in cellular entity, cellular process, and binding; moreover, Kyoto Encyclopedia of Genes and Genomes pathway analyses indicated that genes were involved in gonadotropin-releasing hormone receptor pathway and other signaling pathways. Correlation analysis revealed that miR-31-5p was significantly negatively correlated with clinical indicators of preeclampsia, such as systolic and diastolic pressure, lactate dehydrogenase, and proteinuria. Conclusion We believe that exosome-derived miR-31-5p can serve as an effective and sensitive biomarker to determine the course of preeclampsia in pregnant women.

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Section: Introductionmentioning

confidence: 99%