Hyperlipidemia in streptozocin-diabetic hamsters as a model for human insulin-deficient diabetes: Comparison to streptozocin-diabetic rats

Ebara, Tetsu; Hirano, Tsutomu; Mamo, John; Sakamaki, Ryouichi; Furukawa, Seiichi; Nagano, Seishi; Takahashi, Takehiro

doi:10.1016/0026-0495(94)90096-5

Cited by 29 publications

(14 citation statements)

References 35 publications

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“…A diminuição da área ocupada pelas ilhotas pancreáticas dos hamsteres do grupo D confirma esse quadro, sugerindo que a dose de STZ aplicada foi capaz de provocar diabetes tipo 1 nos animais. Hipercolesterolemia em animais tratados com estreptozotocina resulta da absorção intestinal e da sín-tese aumentadas de colesterol (23). As lipoproteínas de ratos diabéticos são oxidadas e demonstram citotoxicidade, um aspecto que pode ser prevenido pela insulina ou tratamento antioxidante.…”

Section: Discussionunclassified

Efeito da estreptozotocina sobre os perfis glicêmico e lipídico e o estresse oxidativo em hamsters

Silva

Lima

Pedrosa

2011

Arq Bras Endocrinol Metab

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RESUMOObjetivo: Este estudo avaliou os efeitos da estreptozotocina nos perfis glicêmico e lipídico e marcadores de estresse oxidativo em hamsteres. Materiais e métodos: Hamsteres machos Golden Syrian foram divididos em dois grupos: grupo diabético (D), que recebeu uma única injeção de estreptozotocina (STZ -50 mg/kg), e grupo controle (C), que recebeu injeção de tampão citrato. Os animais foram eutanasiados após 10 dias de experimento e o sangue, o fígado e rins foram coletados. Resultados: O grupo diabético apresentou níveis maiores de glicose, triacilgliceróis e colesterol séricos e maior concentração de substâncias reativas ao ácido tiobarbitúrico (TBARs) no fígado e nos rins. Também apresentou significativo aumento da concentração de glutationa no fígado e menores atividades da paraoxonase e do superóxido dismutase. Conclusão: Hamsteres fornecem um bom modelo para o diabetes melito do tipo I e estresse oxidativo, similar ao da síndrome humana, e poderão ser adequados para a análise de compostos antidiabéticos. Arq Bras Endocrinol Metab. 2011;55(1):46-53 Descritores Hamsteres; estreptozotocina; estresse oxidativo; antioxidantes ABSTRACT Objective: This study evaluated the effects of streptozotocin on glycemic and lipid profiles and oxidative stress status in hamsters. Materials and methods: Male Golden Syrian hamsters were divided in diabetic group (D) which received a streptozotocin single injection (STZ -50 mg/kg), and control group (C) which received a single injection of the vehicle citrate buffer. Animals were euthanized after 10 days of experiment and blood, liver and kidneys were collected. Results: The diabetic group had higher levels of glucose, triacylglycerols and cholesterol in serum and thiobarbituric acid reactive substances (TBARS) concentration increased in the liver and kidneys. Diabetes induced a significant increase in glutathione concentration in the liver and decreased paraoxonase and superoxide dismutase activities. Conclusion: Hamsters provide a novel animal model for diabetes mellitus and oxidative stress, similar to the human syndrome, which may be suitable for the testing of antidiabetic compounds. Arq Bras Endocrinol Metab. 2011;55 (1) InTRODUçãO O termo diabetes melito (DM) é uma desordem metabólica de múltiplas etiologias caracterizadas por hiperglicemia crônica com distúrbios no metabolismo de carboidratos, lipídios e proteínas, como resultado do defeito na secreção de insulina ou em sua ação ou ambos (1). Há mais de 250 milhões de pessoas em todo o mundo com diabetes e estima-se que a cada 5 segundos surja um novo caso (2). O diabetes pode gerar complicações como retinopatias, nefropatias, neuropatias e distúrbios endócrinos. O papel do estresse oxidativo nessas complicações tem sido alvo de muito interesse (3,4).

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Section: Discussionunclassified

Efeito da estreptozotocina sobre os perfis glicêmico e lipídico e o estresse oxidativo em hamsters

Silva

Lima

Pedrosa

2011

Arq Bras Endocrinol Metab

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“…In fact, lipoprotein electrophoresis revealed obvious increases in chylomicron and abnormal fractions as well as the LDL fraction. It is noted that apo B-48 containing lipoproteins are exclusively of intestinal origin in Syrian hamsters [4,13] and the chyromicron level was high even though blood was collected during overnight fasting in this model. The lipoprotein changes in this model could not simply be classified as hyperlipidemia in human patients, and further studies are needed to clarify the mechanisms.…”

Section: Discussionmentioning

confidence: 99%

“…In Syrian hamsters, apolipoprotein (apo) B-48 containing lipoproteins is exclusively of intestinal origin and apoB-100 is exclusively of hepatic origin, which is analogous to the condition in humans but not in mice or rats [4,13]. Syrian hamsters of the APA strain (APA hamsters) have recently been shown to have atheromatous lesions in the aortic arches under diabetic conditions induced by a single injection of streptozotocin (SZ) [62].…”

Section: Introductionmentioning

confidence: 99%

APA Hamster Model for Diabetic Atherosclerosis. 2. Analysis of Lipids and Lipoproteins.

et al. 2000

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Syrian hamsters of the APA strain (APA hamsters) have recently been shown to have atheromatous lesions in the aortic arches under diabetic condition induced by a single injection of streptozotocin (SZ). In that model, fatty streaks, which are the initial lesions of atherogenesis, develop by 6 weeks after the injection (WAI). In this study, we evaluated plasma lipid concentrations and lipoprotein profiles in diabetic APA hamsters at 6 WAI to reveal the early stage of atherogenesis clinicopathologically. As a result, by biochemical analysis, hyperglycemic APA hamsters showed signs of hypercholesterolemia and hypertriglyceridemia. Low-density lipoprotein (LDL) cholesterol significantly increased, but high-density lipoprotein (HDL) cholesterol significantly decreased. Agarose gel electrophoresis showed an obvious increase in the fractions of chylomicron, LDL and abnormal lipoprotein. Plasma LDL in diabetic animals was in a state more susceptible to oxidization. In addition, a significant increase in glycated LDL was also found in the diabetic animals by enzyme linked immunosorbent assay (ELISA). Moreover, lipid peroxidation product (4-hydroxynonenal (4 HNE))-adducted proteins and advanced glycation end-products (AGE) were immunohistochemically detected in the foam cells of the fatty streaks. These results revealed that diabetic APA hamsters had hyperlipidemia characterized by increases in chylomicron, LDL and abnormal lipoprotein, and suggested that oxidized LDL and/or glycated LDL might be actively uptaken by macrophages and play an important role in the initial stage of atherogenesis.

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“…Non-esterified fatty acids (NEFA) were quantified using a Wako NEFA C test kit (Alpha laboratories, London, UK) based on an acyl-coA synthetase/acyl coA oxidase enzymatic colorimetric method. LDL-cholesterol is present at very low concentrations in these rats [24] and measurements were not performed.…”

Section: Methodsmentioning

confidence: 99%

Endothelial dysfunction in streptozotocin-diabetic rats is not reversed by dietary probucol or simvastatin supplementation

et al. 1998

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Insulin-dependent diabetes mellitus (IDDM) is associated with an increased risk of hypertension, atherosclerosis and disorders of the microcirculation and there is increasing evidence to suggest that vascular endothelial dysfunction may play a major role. Impaired endothelium-dependent vasodilatation to acetylcholine (ACh) has been reported in aorta [1], small mesenteric [2] and femoral [3] arteries of the streptozotocin (STZ)-rat, and in diabetic patients [4,5], and is generally associated with reduced availability of nitric oxide (NO). The factors responsible for altered NO synthesis or release are unknown and, although hyperglycaemia undoubtedly contributes [6], there is increasing evidence that oxidative stress coupled with dyslipidaemia [7,8] could also be important determinants of endothelial dysfunction. Free radicals (which may be generated as a consequence of glucose-induced activation of cyclooxygenase, au- Diabetologia (1998) 41: 157±164 Endothelial dysfunction in streptozotocin-diabetic rats is not reversed by dietary probucol or simvastatin supplementation

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Hyperlipidemia in streptozocin-diabetic hamsters as a model for human insulin-deficient diabetes: Comparison to streptozocin-diabetic rats

Cited by 29 publications

References 35 publications

Efeito da estreptozotocina sobre os perfis glicêmico e lipídico e o estresse oxidativo em hamsters

Efeito da estreptozotocina sobre os perfis glicêmico e lipídico e o estresse oxidativo em hamsters

APA Hamster Model for Diabetic Atherosclerosis. 2. Analysis of Lipids and Lipoproteins.

Endothelial dysfunction in streptozotocin-diabetic rats is not reversed by dietary probucol or simvastatin supplementation

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