Hyperlayer hollow-fiber flow field-flow fractionation of cells

Reschiglian, Pierluigi; Zattoni, Andrea; Roda, Barbara; Cinque, Leonardo; Melucci, Dora; Min, Byung Ryul; Moon, Myeong Hee

doi:10.1016/s0021-9673(02)01458-9

Cited by 60 publications

(52 citation statements)

References 35 publications

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“…Hollow-fiber flow field-flow fractionation (HF FlFFF), a variant of FlFFF techniques, was first described in a few papers on separation of spherical latex standard particles [13,14], and recently it has been gaining interest as an efficient separation technique for particles [15,16], proteins [17][18][19], and cells [20,21]. Unlike the conventional FlFFF channel system having a rectangular channel design, HF FlFFF is operated in a cylindrical channel utilizing a hollow-fiber membrane module which is very simple in assembly and disposable.…”

Section: Introductionmentioning

confidence: 99%

“…In case of separating particles larger than typically 1 lm (in some cases, this value is as small as 0.4-0.8 lm in typical flow FFF [22]), particle elution follows the principle of hyperlayer mode of separation in which a large particle is lifted further away from the wall than the small one due to the action of hydrodynamic lift forces, and thus a large particle is eluted earlier than a small one [16,23,24]. In recent studies, HF FlFFF has been applied to the investigation of membrane effect on resolution and selectivity of hyperlayer separation of polystyrene (PS) standard particles [16] and for the characterization of supramicron-sized cells and bacteria [21]. Since particle retention in FlFFF is regardless of density or mass, separation of particles in HF FlFFF is influenced by the hydrodynamic radius only.…”

Section: Introductionmentioning

confidence: 99%

“…Since particle retention in FlFFF is regardless of density or mass, separation of particles in HF FlFFF is influenced by the hydrodynamic radius only. Therefore, particle size or size distribution can be obtained if a proper calibration in hollow-fiber flow/hyperlayer field-flow fractionation (HF Fl/HyFFF) is established with standard particles [17,21].…”

Section: Introductionmentioning

confidence: 99%

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Hollow-fiber flow/hyperlayer field-flow fractionation for the size characterization of airborne particle fractions obtained by SPLITT fractionation

Kim¹,

Oh²,

Moon³

2006

J. Sep. Sci.

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Hollow-fiber flow field-flow fractionation (HF FlFFF) was applied for the separation and size characterization of airborne particles which were collected in a municipal area and prefractionated into four different-diameter intervals >5.0, 2.5-5.0, 1.5-2.5, <1.5 microm) by continuous split-flow thin (SPLIIT) fractionation. Experiments demonstrated the possibility of utilizing a hollow-fiber module for the high-performance separation of supramicron-sized airborne particles at steric/hyperlayer operating mode of HF FlFFF. Eluting particles during HF FlFFF separation were collected at short time intervals (approximately 10 s) for the microscopic examination. It showed that particle size and size distributions of all SPLITT fractions of airborne particles can be readily obtained using a calibration and that HF FlFFF can be utilized for the size confirmation of the sorted particle fraction during SPLITT fractionation.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Hollow-fiber flow/hyperlayer field-flow fractionation for the size characterization of airborne particle fractions obtained by SPLITT fractionation

Kim¹,

Oh²,

Moon³

2006

J. Sep. Sci.

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“…4 This technique employs a piece (20-25 cm in length) of submillimiter-sized hollow-fiber membrane (HF) for microdialysis, which is used as microcolumn channel for FlFFF. [5][6][7][8] HF microdialysis had been successfully coupled with MS to separate protein samples into two fractions, one being the permeate containing low molecular weight compounds, and the other being the retentate containing the proteins. 9,10 When a HF membrane is used for FlFFF, also the retentate components (proteins) are separated, which is an advantage for their further MS analysis.…”

mentioning

confidence: 99%

“…from Upchurch Scientific, Oak Harbor, WA) are positioned at the HF inlet and outlet. [6][7][8] Polysulfone or polyacrylonitrile HF membranes with 10,000 to 30,000 Da pore cut-off (SK Chemicals, Seoul, Korea; Chemicore, Daejeon, Korea) have been employed. The HF FlFFF channel is inserted into a properly modified HPLC system.…”

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confidence: 99%

Hollow‐Fiber Flow Field‐Flow Fractionation: A Gentle Separation Method for Mass Spectrometry of Native Proteins

et al. 2006

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Low-impact ionization sources like electrospray ionization (ESI) and matrix-assisted, laser desorption/ionization (MALDI) equipped with time-of-flight (TOF) mass analyzers provide intact protein analysis over a very wide molar mass range. ESI/TOFMS provides also indications on the higher-order structure of intact proteins and non-covalent protein complexes. However, direct analysis of intact proteins mixtures in real samples shows limited success, mainly because spectra become very complex to interpret. This is also due to sample contaminants, and to the mechanism of competitive ionization in ESI or MALDI. Rapid and efficient sample clean-up and separation methods can significantly enhance the power of TOFMS for intact protein analysis. However, if protein native conditions want to be maintained, the methods should affect neither the three-dimensional structure nor the non-covalent chemistry of the proteins. Reversed-phase (RP) HPLC, size-exclusion chromatography (SEC), and capillary zone electrophoresis (CZE) are on-line or off-line coupled to ESI/TOFMS or MALDI/TOFMS. In fact, these separation methods often show limitations when applied to the analysis of native proteins. Organic modifiers and saline buffers are required in the case of RP HPLC or CZE. They can induce protein degradation or affect ionization when MS is performed after separation. High voltages used in CZE can contribute to alter proteins from their native form. In the case of high molar mass proteins, SEC is scarcely selective, and barely able to detect protein aggregates. Sample entanglement/adsorption on the stationary phase can also occur.

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Rapid separation of bacteria from blood—review and outlook

Pitt

Alizadeh

Husseini

et al. 2016

Biotechnology Progress

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The high morbidity and mortality rate of bloodstream infections involving antibiotic-resistant bacteria necessitate a rapid identification of the infectious organism and its resistance profile. Traditional methods based on culturing the blood typically require at least 24 h, and genetic amplification by PCR in the presence of blood components has been problematic. The rapid separation of bacteria from blood would facilitate their genetic identification by PCR or other methods so that the proper antibiotic regimen can quickly be selected for the septic patient. Microfluidic systems that separate bacteria from whole blood have been developed, but these are designed to process only microliter quantities of whole blood or only highly diluted blood. However, symptoms of clinical blood infections can be manifest with bacterial burdens perhaps as low as 10 CFU/mL, and thus milliliter quantities of blood must be processed to collect enough bacteria for reliable genetic analysis. This review considers the advantages and shortcomings of various methods to separate bacteria from blood, with emphasis on techniques that can be done in less than 10 min on milliliter-quantities of whole blood. These techniques include filtration, screening, centrifugation, sedimentation, hydrodynamic focusing, chemical capture on surfaces or beads, field-flow fractionation, and dielectrophoresis. Techniques with the most promise include screening, sedimentation, and magnetic bead capture, as they allow large quantities of blood to be processed quickly. Some microfluidic techniques can be scaled up.

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Hyperlayer hollow-fiber flow field-flow fractionation of cells

Cited by 60 publications

References 35 publications

Hollow-fiber flow/hyperlayer field-flow fractionation for the size characterization of airborne particle fractions obtained by SPLITT fractionation

Hollow-fiber flow/hyperlayer field-flow fractionation for the size characterization of airborne particle fractions obtained by SPLITT fractionation

Hollow‐Fiber Flow Field‐Flow Fractionation: A Gentle Separation Method for Mass Spectrometry of Native Proteins

Rapid separation of bacteria from blood—review and outlook

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