Hyperimmune Bovine Colostrum as a Novel Therapy to Combat Clostridium difficile Infection

Sponseller, Jerlyn K.; Steele, Jennifer; Schmidt, Diane; Kim, Hyeun Bum; Beamer, Gillian; Sun, Xingmin; Tzipori, Saul

doi:10.1093/infdis/jiu605

Cited by 46 publications

(49 citation statements)

References 53 publications

(61 reference statements)

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“…The interest in studying the biological properties of bovine colostrum is related to its rich composition in bioactive molecules including growth factors, 1‐3,18 which exert a growth‐promoting or‐inhibitory activity, depending on the target cells. Several studies have demonstrated the safety and effectiveness of bovine colostrum in the treatment of gastrointestinal disorders and in the management of nonhealing wounds 3,7,8,10,15‐17,22,31,32,55,56 . Impaired wound repair can be improved by applying a combination of exogenous growth factors, thanks to their synergistic pro‐healing action in the wounded area 57‐64 .…”

Section: Discussionmentioning

confidence: 99%

“…Bovine colostrum provides passive immunity, protecting newborns from enteric infection, and contributes to the maturation and integrity of the gastrointestinal tract 6‐10 . There is also evidence that it is effective as an adjuvant in the treatment of diseases associated to viral and bacterial infections 3,11‐17 . The growth‐promoting and tissue repair activities of bovine colostrum are due to the rich composition of growth factors, 2,3,7,8,10,18‐23 among which: insulin‐like growth factors (IGF‐I and IGF‐II), involved in cell proliferation and differentiation 3,24,25 ; the epidermal growth factor (EGF) that stimulates gut growth in neonates and gut repair processes in adults 26,27 ; the transforming growth factor‐β (TGF‐β) that has an anti‐inflammatory and anti‐proliferative activity on epithelial cells, and promotes the healing process stimulating the epithelial cell migration at wound sites 19,28 ; the transforming growth factor‐α (TGF‐α) that plays a key role in response to injuries, stimulating gastrointestinal growth and repair, 3 and controls cell migration and differentiation.…”

Section: Introductionmentioning

confidence: 99%

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Bovine colostrum induces the differentiation of human primary keratinocytes

et al. 2020

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Abbreviations: 2D, two-dimensional; 3D, three-dimensional; K1, keratin 1; K5, keratin 5; K16, keratin 16; NHKs, normal human keratinocytes; NHFs, normal human fibroblasts; NMFs, natural moisturizing factors. AbstractBovine colostrum, the first milk secreted by the mammary glands of cows shortly after they have given birth, provides a natural source of bioactive substances helpful to promote tissue development and repair, and to maintain a healthy immune system.Owing to its properties, the use of colostrum in the treatment of human diseases is under investigation. We evaluated the biological activity of colostrum on human primary keratinocytes, focusing on its effects with regard to a proliferation/differentiation balance. Using cellular and molecular approaches, we showed that colostrum favors a cell cycle withdrawal by increasing the expression of p21/WAF1 and p27/ KIP1. It also promotes the transition of keratinocytes from a proliferating to a differentiating state, as assessed by a decrease in keratin 5 and an increase in keratin 16.We demonstrated the ability of colostrum to induce the expression of early and late differentiation markers (keratin 1, involucrin, and filaggrin) and the synthesis of caspase 14 and bleomycin hydrolase, the two main enzymes involved in filaggrin maturation. Moreover, we showed that bovine colostrum is able to promote keratinocyte stratification and terminal differentiation not only in two-dimensional (2D), but also in a more physiological system of three-dimensional (3D) skin equivalents. Finally, we demonstrated that colostrum stimulates cell differentiation through the PI3K/ PLC-γ1/PKCα pathways mainly associated to tyrosine kinase receptors. These results suggest the possibility to benefit from colostrum properties for the treatment of skin diseases characterized by altered differentiation and perturbed barrier function.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Bovine colostrum induces the differentiation of human primary keratinocytes

et al. 2020

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“…Recently, it was proved to alleviate symptoms of CDI in gnotobiotic piglets without detectably affecting normal intestinal microbiota [163]. Whey protein concentrate derived from immunized cow milk was proved to be safe and well tolerated in human subjects with CDI as well [164].…”

Section: Toxin-neutralizing Agentsmentioning

confidence: 99%

A Review of Experimental and Off-Label Therapies for Clostridium difficile Infection

2016

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In spite of increased awareness and the efforts taken to optimize Clostridium difficile infection (CDI) management, with the limited number of currently available antibiotics for C. difficile the halt of this increasing epidemic remains out of reach. There are, however, close to 80 alternative treatment methods with controversial anti-clostridial efficacy or in experimental phase today. Indeed, some of these therapies are expected to become acknowledged members of the recommended anti-CDI arsenal within the next few years. None of these alternative treatment methods can respond in itself to all the major challenges of CDI management, which are primary prophylaxis in the susceptible population, clinical cure of severe cases, prevention of recurrences, and forestallment of asymptomatic C. difficile carriage and in-hospital spread. Yet, the greater the variety of treatment choices on hand, the better combination strategies can be developed to reach these goals in the future. The aim of this article is to provide a comprehensive summary of these experimental and currently off-label therapeutic options.

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“…Bacteriocins and specific antibodies are further alternatives. A hyperimmune bovine colostrum showed in a recent animal trial treatment effects against CDI without affecting the gut microbiota composition (Sponseller et al ., 2015). …”

Section: Eliminating Undesired Bacteria From the Gut Microbiomementioning

confidence: 99%

“…A hyperimmune bovine colostrum showed in a recent animal trial treatment effects against ª 2016 The Author. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology, Microbial Biotechnology, 9, 553-563 CDI without affecting the gut microbiota composition (Sponseller et al, 2015).…”

Section: Bacteriophagesmentioning

confidence: 99%

Biome engineering‐2020

Brüssow

2016

Microbial Biotechnology

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SummaryThe gut microbiome research is going from a descriptive into an intervention phase. To optimize beneficial microbe–host interaction, we need to understand how to steer the system by modulating the nutrient input with which the system is literally fed (e.g. diets, fibres, prebiotics, human milk oligosaccharides), and we must learn how to modulate the composition of the gut microbiota by adding beneficial microbes (e.g. probiotics, faecal transplants) and by eliminating disturbing microbial members using, for example, bacteriophages in this highly complex ecosystem. The current status of the field is reviewed together with an outlook what might be expected until 2020, highlighting obstacles to progress and possible solutions to these problems.

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Hyperimmune Bovine Colostrum as a Novel Therapy to Combat Clostridium difficile Infection

Abstract: HBC provides an oral, cost-effective, and safe alternative to antibiotic therapy for CDI. By preserving intestinal microbiota, HBC may be more efficacious than antibiotics. Additional studies are warranted to establish HBC as a viable immunotherapeutic agent for human use against CDI.

Cited by 46 publications

References 53 publications

Bovine colostrum induces the differentiation of human primary keratinocytes

Bovine colostrum induces the differentiation of human primary keratinocytes

A Review of Experimental and Off-Label Therapies for Clostridium difficile Infection

Biome engineering‐2020

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