Hypericum adenotrichum SPACH‘UN KALLUS İNDÜKSİYONU VE ADVENTİF SÜRGÜN REJENERASYONU

Abstract

In this study, A protocol to callus formation and adventitious shoot regeneration of Hypericum adenotrichum Spach via direct or indirect organogenesis has been described.Callus induction was carried out by using leaves which were collected from their native environment. The maximum callus induction frequency has been observed on MS medium containing 4 mgL -1 BA and 0.2 mgL -1 NAA. These calli were not induced to shoot regeneration. Shoot formation was obtained by transferring of the callus from only BA-containing MS medium to MS medium containing 0.5 mgL -1 KIN. Maximum shoot number was observed when the callus was transferred from callus induction medium containing 3 mgL -1 BA to medium containing 0.5 mgL -1 KIN. Direct shoot formation developed on leaf explants of Hypericum adenotrichum on MS medium containing KIN alone. The highest shoot number of per explant was observed on MS medium with 1 mgL -1 KIN.