“…The GlycoID tools presented here are meant to be applied in live cell settings in order to capture the complex intracellular protein milieu (Danielsson & Oliveberg, 2017). This live cell capability enables O-GlcNAc-directed labeling under dynamic cellular conditions provided by the users, allowing manipulation of variables known to influence O-GlcNAcylation such as nutrient levels (Ayodeji et al, 2023), signaling pathway activation (Liu et al, 2022), cellular stressors (Martinez et al, 2017), or disease mutations (Konzman et al, 2020). Strategic considerations, therefore, include which cell lines to use as well as which conditions to study or compare O-GlcNAc states between using GlycoID labeling (Fig.…”