Hyperglycemia affects neuronal differentiation and Nestin, FOXO1, and LMO3 mRNA expression of human Wharton's jelly mesenchymal stem cells of children from diabetic mothers

Domínguez-Castro, Mauricio; Domínguez-Galicia, Arely; Pérez-Pérez, Oscar; Hernández-Pineda, Jessica; Mancilla-Herrera, Ismael; Rodríguez-Cruz, Leonor; González-Torres, María Cristina; Montoya-Estrada, Araceli; Reyes-Muñoz, Enrique; Romo-Yáñez, José

doi:10.1016/j.bbrc.2022.11.029

Cited by 3 publications

(2 citation statements)

References 36 publications

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“…Its role in the chondrogenic, osteogenic, adipogenic and neurogenic differentiation of MSCs has already been reported [ 59 , 60 , 61 , 62 , 63 , 64 ]. In terms of neurogenic differentiation, Dominguez-Castro et al [ 65 ] reported the role of FOXO1 in WJ-MSCs specifically, revealing an elevated level of FOXO1 in WJ-MSCs during neuronal differentiation both in normoglycemic pregnancies and in pregestational diabetes mellitus. The current results are consistent with previous findings and provide evidence for FOXO1′s involvement in the in vitro differentiation of WJ-MSCs.…”

Section: Discussionmentioning

confidence: 99%

Apoptosis Related Human Wharton’s Jelly-Derived Stem Cells Differentiation into Osteoblasts, Chondrocytes, Adipocytes and Neural-like Cells—Complete Transcriptomic Assays

Stefańska

Němcová

Blatkiewicz

et al. 2023

IJMS

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Wharton’s jelly-derived mesenchymal stem cells (WJ-MSCs) exhibit multilineage differentiation potential, adhere to plastic, and express a specific set of surface markers—CD105, CD73, CD90. Although there are relatively well-established differentiation protocols for WJ-MSCs, the exact molecular mechanisms involved in their in vitro long-term culture and differentiation remain to be elucidated. In this study, the cells were isolated from Wharton’s jelly of umbilical cords obtained from healthy full-term deliveries, cultivated in vitro, and differentiated towards osteogenic, chondrogenic, adipogenic and neurogenic lineages. RNA samples were isolated after the differentiation regimen and analyzed using an RNA sequencing (RNAseq) assay, which led to the identification of differentially expressed genes belonging to apoptosis-related ontological groups. ZBTB16 and FOXO1 were upregulated in all differentiated groups as compared to controls, while TGFA was downregulated in all groups. In addition, several possible novel marker genes associated with the differentiation of WJ-MSCs were identified (e.g., SEPTIN4, ITPR1, CNR1, BEX2, CD14, EDNRB). The results of this study provide an insight into the molecular mechanisms involved in the long-term culture in vitro and four-lineage differentiation of WJ-MSCs, which is crucial to utilize WJ-MSCs in regenerative medicine.

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Section: Discussionmentioning

confidence: 99%

Apoptosis Related Human Wharton’s Jelly-Derived Stem Cells Differentiation into Osteoblasts, Chondrocytes, Adipocytes and Neural-like Cells—Complete Transcriptomic Assays

Stefańska

Němcová

Blatkiewicz

et al. 2023

IJMS

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“…Although a direct association between maternal diabetes and TCS has not been established, it is well known that maternal diabetes can lead to infant neural tube defects (NTDs). A general understanding is that a high glucose concentration prenatally leads to neuronal cell death via regulation of genes and thus interrupts closing of the neural tube [11]. A similar process could be leading to TCS.…”

Section: Discussionmentioning

confidence: 99%

Prospective Screening for Sacral Agenesis in Pregestational Diabetes: A Pilot Study

Gajagowni

Mazuru

Hegstrom

et al. 2023

Preprint

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Background Offspring of diabetic mothers have a higher incidence of birth defects. One such defect is sacral agenesis. It is a rare developmental abnormality consisting of the absence of part or all of the sacrum. Offspring of mothers with PGDM have a higher risk of sacral agenesis compared to offspring of mothers with no diabetes. Despite this, there are currently no guidelines to routinely screen offspring of diabetic mothers for sacral agenesis. Research has shown that early intervention for sacral agenesis decreases the risk for future urinary and musculoskeletal complications and thus improves overall prognosis. Objective Determine the utility of prospective spinal ultrasound in infants of mothers with PGDM for the diagnosis of sacral agenesis. Methods This prospective observational pilot study was completed at Women’s and Children’s Hospital in Columbia, Missouri between May 1, 2020 and December 30, 2022. Infants born to mothers with PGDM and with normal spinal examinations were included. A total of 22 mother-infant dyads were enrolled in the study and prospectively screened with spinal ultrasound. The study was registered on ClinicalTrials.gov (Identifier-NCT05033275). Results Twenty-two spinal ultrasounds were performed over the course of this study with three (14%) resulting in abnormal findings that required further imaging. Follow-up with magnetic resonance imaging found one case of tethered cord syndrome. Conclusion Prospective screening in infants of mothers with PGDM found no cases of sacral agenesis but did identify tethered cord syndrome. This finding suggests that risk stratified screening of mothers with diabetes might be a reasonable approach to care.

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Insights into the Therapeutic Potential of Phytoconstituents as an Epigenetic Modulators in Diabetic Nephropathy

Thapa,

Khan,

Grewal

et al. 2023

Curr. Pharmacol. Rep.

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Hyperglycemia affects neuronal differentiation and Nestin, FOXO1, and LMO3 mRNA expression of human Wharton's jelly mesenchymal stem cells of children from diabetic mothers

Cited by 3 publications

References 36 publications

Apoptosis Related Human Wharton’s Jelly-Derived Stem Cells Differentiation into Osteoblasts, Chondrocytes, Adipocytes and Neural-like Cells—Complete Transcriptomic Assays

Apoptosis Related Human Wharton’s Jelly-Derived Stem Cells Differentiation into Osteoblasts, Chondrocytes, Adipocytes and Neural-like Cells—Complete Transcriptomic Assays

Prospective Screening for Sacral Agenesis in Pregestational Diabetes: A Pilot Study

Insights into the Therapeutic Potential of Phytoconstituents as an Epigenetic Modulators in Diabetic Nephropathy

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