Hyperexpression of epidermal growth factor receptors in granulosa cells from women with polycystic ovary syndrome

Almahbobi, Ghanim; Misajon, Aileen; Hutchinson, Paul; Lolatgis, Nicholas; Trounson, Alan

doi:10.1016/s0015-0282(98)00252-0

Cited by 18 publications

(6 citation statements)

References 35 publications

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“…In the human ovary, EGF is found in the FF, regulating follicular development and oocyte meiotic maturation competence via EGFR signaling transduction system in the CCs (Westergaard and Andersen, 1989; Almahbobi et al ., 1998; Jamnongjit et al ., 2005; Hsieh et al ., 2009). IVM studies show that exposure of the cumulus–oocyte complex (COC) to EGF stimulates CC expansion and improves the nuclear and cytoplasmic maturation of oocytes from the metaphase I (MI) to metaphase II (MII) stage in both humans and other mammals (Goud et al ., 1998; Smitz et al ., 1998; De La Fuente et al ., 1999), significantly facilitating fertilization and embryo development (Singh et al ., 1997; Goff et al ., 2001).…”

Section: Intra-ovarian Factorsmentioning

confidence: 99%

“…EGF inhibits estrogen synthesis in GCs, which may explain why EGF blocks antral follicle growth and results in follicular arrest in PCOS patients (Artini et al ., 2007). Therefore, it is hypothesized that a disruption in the regulatory mechanisms of EGF synthesis and/or physiological function mediated by EGFR may cause anovulatory infertility in women with PCOS (Almahbobi and Trounson, 1996; Almahbobi et al ., 1998). Whether an elevated level of EGF in FF is correlated to oocyte quality and embryonic developmental competence is still unclear.…”

Section: Intra-ovarian Factorsmentioning

confidence: 99%

See 1 more Smart Citation

Extra- and intra-ovarian factors in polycystic ovary syndrome: impact on oocyte maturation and embryo developmental competence

Qiao

Feng

2010

Human Reproduction Update

396

314

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BACKGROUNDPolycystic ovary syndrome (PCOS) is a common metabolic dysfunction and heterogeneous endocrine disorder in women of reproductive age. Although patients with PCOS are typically characterized by increased numbers of oocytes retrieved during IVF, they are often of poor quality, leading to lower fertilization, cleavage and implantation rates, and a higher miscarriage rate.METHODSFor this review, we searched the database MEDLINE (1950 to January 2010) and Google for all full texts and/or abstract articles published in English with content related to oocyte maturation and embryo developmental competence.RESULTSThe search showed that alteration of many factors may directly or indirectly impair the competence of maturating oocytes through endocrine and local paracrine/autocrine actions, resulting in a lower pregnancy rate in patients with PCOS. The extra-ovarian factors identified included gonadotrophins, hyperandrogenemia and hyperinsulinemia, although intra-ovarian factors included members of the epidermal, fibroblast, insulin-like and neurotrophin families of growth factors, as well as the cytokines.CONCLUSIONSAny abnormality in the extra- and/or intra-ovarian factors may negatively affect the granulosa cell–oocyte interaction, oocyte maturation and potential embryonic developmental competence, contributing to unsuccessful outcomes for patients with PCOS who are undergoing assisted reproduction.

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Section: Intra-ovarian Factorsmentioning

confidence: 99%

Section: Intra-ovarian Factorsmentioning

confidence: 99%

Extra- and intra-ovarian factors in polycystic ovary syndrome: impact on oocyte maturation and embryo developmental competence

Qiao

Feng

2010

Human Reproduction Update

396

314

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“…Tgf-β2 knock out mice study showed multiple developmental defects, including cardiopulmonary, skeletal, ocular, and urogenital system defects 83 . In women, dysregulation of this transforming growth factor circuitry was associated with PCOS 84,85 and fertility problems 86 . These findings may point to potential roles of CBX2 .…”

Section: Discussionmentioning

confidence: 99%

The transcriptional regulator CBX2 and ovarian function: A whole genome and whole transcriptome approach

Bouazzi

Sproll

Eid

et al. 2019

Sci Rep

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The chromobox homolog 2 (CBX2) was found to be important for human testis development, but its role in the human ovary remains elusive. We conducted a genome-wide analysis based on DNA adenine methyltransferase identification (DamID) and RNA sequencing strategies to investigate CBX2 in the human granulosa cells. Functional analysis revealed that CBX2 was upstream of genes contributing to ovarian function like folliculogenesis and steroidogenesis (i.e. ESR1, NRG1, AKR1C1, PTGER2, BMP15, BMP2, FSHR and NTRK1/2). We identified CBX2 regulated genes associated with polycystic ovary syndrome (PCOS) such as TGFβ, MAP3K15 and DKK1, as well as genes implicated in premature ovarian failure (POF) (i.e. POF1B, BMP15 and HOXA13) and the pituitary deficiency (i.e. LHX4 and KISS1). Our study provided an excellent opportunity to identify genes surrounding CBX2 in the ovary and might contribute to the understanding of ovarian physiopathology causing infertility in women.

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“…Epidermal growth factor (EGF) is a growth factor that plays an important role in the regulation of cell proliferation and differentiation [27]. In the human oocyte, EGF is found in the follicular microenvironment (Figure 2) regulating the development and maturation of oocytes [27][28][29].…”

Section: Epidermal Growth Factor (Egf)mentioning

confidence: 99%

“…Therefore, it is assumed that an alteration of the regulation of EGF synthesis and/or its action mediated through its specific receptor (EGF-R) can cause anovulatory infertility in women with polycystic ovary syndrome [28].…”

Section: Epidermal Growth Factor (Egf)-like Growth Factors In the Hum...mentioning

confidence: 99%

In vitro Maturation (IVM) Perspectives

Alsadi¹

2023

IVF Technologies and Infertility - Current Practices and New Perspectives

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The basic concept of in vitro maturation (IVM) of oocytes in practice clinic consists of the collection of immature oocytes from small antral follicles before spontaneous ovulation and then left to mature in vitro. IVM is based on the observations of Pincus and Enzmann in 1935 and Edwards in 1965, which highlighted the spontaneous nuclear maturation of the follicles when they were removed from their ovarian context and matured in vitro, and these first discoveries of in vitro folliculogenesis laid the foundations for the present research on the technique of in vitro maturation. In vitro folliculogenesis represents not only the possibility of extending the availability of female gametes in terms of the number of fertilizable oocytes but also a model within which to understand the complex mechanisms that regulate the synergistic development between the follicle and the female gamete. Deeper understanding of the complex orchestration of maturation, nuclear and cytoplasmic, of the oocyte based on research of bases on animal oocytes allowed the clinical application of the IVM technique to begin in reproductive medicine.

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Hyperexpression of epidermal growth factor receptors in granulosa cells from women with polycystic ovary syndrome

Cited by 18 publications

References 35 publications

Extra- and intra-ovarian factors in polycystic ovary syndrome: impact on oocyte maturation and embryo developmental competence

Extra- and intra-ovarian factors in polycystic ovary syndrome: impact on oocyte maturation and embryo developmental competence

The transcriptional regulator CBX2 and ovarian function: A whole genome and whole transcriptome approach

In vitro Maturation (IVM) Perspectives

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