Hypercalcemia, necrotizing pancreatitis and bone lesions: a benign cause

Rauber, Conrad; Kihm, Lars; Merle, Uta

doi:10.11138/ccmbm/2017.14.2.245

Cited by 2 publications

(2 citation statements)

References 7 publications

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“… 14 Especially in bones, the excess calcium in blood was leached from bones, subsequently it can cause bone pain and muscle weakness. 15 Therefore, it is necessary to supplement the appropriate concentration of calcium needed physiologically. In this study, we aim to elucidate the role of high calcium concentrations in controlling hPDLSC differentiation mainly through Runx2 or osterix regulation.…”

Section: Introductionmentioning

confidence: 99%

High concentrations of calcium suppress osteogenic differentiation of human periodontal ligament stem cells in vitro

Han

2021

Journal of Dental Sciences

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Background/purpose Periodontal ligament stem cells (PDLSCs)-based regeneration therapy has received attention for its potential alternative applications in hard tissue and tooth. However, the environmental diversity of oral cavity that regulates PDLSCs differentiation has made it difficult to develop. Therefore, we investigated how high calcium concentrations in the oral environment influence osteogenic differentiation of human PDLSCs (hPDLSCs). Materials and methods hPDLSCs collected from human molars were isolated and cultured with CaCl 2 . First, multi lineage differentiation potentials to osteogenic, chondrogenic, and adipogenic cells were investigated. Then, the effects of CaCl 2 on both alkaline phosphatase (ALP) activity and bone mineralization were analyzed and the expression of mRNA and protein for osteogenic marker was explored. Further, luciferase assay was performed to evaluate CaCl 2 could regulate the transcriptional activity on osteogenic differentiation in hPDLSCs Results CaCl 2 treatment at normal to high concentrations showed similar suppression of ALP activity, while mineralized nodule formation was decreased by CaCl 2 treatment dose-dependently without affecting proliferation or cytotoxicity in hPDLSCs. We also observed that CaCl 2 treatment repressed the mRNA expression and protein abundance of osteogenic genes and transcriptional factors. Notably, repression of the Runx2 level was significant, and CaCl 2 treatment inhibited Runx2-mediated transcriptional activity on the osteoblast-specific element (OSE) and ALP promoters. Conclusion High concentrations of calcium negatively regulate osteogenic differentiation of hPDLSCs, by repressing osteogenic gene expressions and transcriptional activity. Therefore, these conditions may be applicable to determine the physiologically appropriate concentration of calcium.

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Section: Introductionmentioning

confidence: 99%

High concentrations of calcium suppress osteogenic differentiation of human periodontal ligament stem cells in vitro

Han

2021

Journal of Dental Sciences

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“…A pancreatite ocorre nos estágios avançados da afecção paratireoidiana, o que explica sua menor incidência em países desenvolvidos, onde o HPP é diagnosticado precocemente 7 . O risco de desenvolvimento de pancreatite é maior quanto mais elevados forem os níveis séricos de cálcio 4,3,8 . Valores acima 14 mg/dl elevam em 25% o risco de pancreatite 3 .…”

Section: Relato De Casounclassified

Hiperparatireoidismo primário: relato de caso sobre uma apresentação rara de pancreatite aguda

Dias

Kuroda

Dib

et al. 2020

ACS

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Introdução: A pancreatite aguda (PA) é uma condição inflamatória comum nos centros médicos com uma incidência anual mundial de 4,9 - 73,4 casos por 100.000 habitantes e tem como principais causas o cálculo biliar (40-70%) e o etilismo (25-35%) seguidas de outras condições incomuns, entre elas a hipertrigliceridemia e a hipercalcemia. A PA devido a hipercalcemia, embora rara, pode representar dificuldade diagnóstica e, em muitos casos, ser diagnosticada tardiamente. Objetivo: Relatar um caso raro de pancreatite aguda causada por hipercalcemia. Relato de Caso: Paciente internado quatro vezes em três anos com queixa de dor abdominal. No momento inicial foi feito o diagnóstico de pancreatite alcoólica reforçado pelo histórico pessoal de etilismo crônico. Na última internação do paciente foram investigados os níveis séricos de cálcio total e paratormônio (PTH), com valores respectivos de 12,2 mmol/L e 178,8 pg/mL, sugerindo possível hiperparatireoidismo primário (HPP). Confirmou-se a hipótese com a cintilografia de paratireoide e o paciente foi submetido à paratireoidectomia esquerda e tireoidectomia parcial homolateral, apresentando melhora dos níveis séricos de PTH no pós-operatório, assim como do seu quadro de PA. Conclusão: A dosagem de cálcio sérico em pacientes com quadro de PA pode ser benéfica para o diagnóstico, instituiçãoprecoce de tratamento e controle de recidivas em pacientes com HPP.

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Hypercalcemia, necrotizing pancreatitis and bone lesions: a benign cause

Cited by 2 publications

References 7 publications

High concentrations of calcium suppress osteogenic differentiation of human periodontal ligament stem cells in vitro

High concentrations of calcium suppress osteogenic differentiation of human periodontal ligament stem cells in vitro

Hiperparatireoidismo primário: relato de caso sobre uma apresentação rara de pancreatite aguda

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