Hyperbranched polyglycerols on the nanometer and micrometer scale

Steinhilber, Dirk; Seiffert, Sebastian; Heyman, John A.; Paulus, Florian; Weitz, David A.; Haag, Rainer

doi:10.1016/j.biomaterials.2010.10.010

Cited by 102 publications

(91 citation statements)

References 38 publications

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“…A number of recent studies considered hyperbranched polymers for various biomedical-related applications such as designing bioresistive surfaces, gene theraphy, drug delivery containers, bioadhesives, biospecific binders, cell encapsulation, and others, [154][155][156][157][158][159] with some of them to be summarized below. For instance, Haag et al prepared protein-resistant, highly branched PG SAMs on gold substrates by a simple chemical modification of these readily available polymers with a surface-active disulfide (thioctic acid) linker group.…”

Section: Hyperbranched Polymers For Biomedical Applicationsmentioning

confidence: 99%

Assembling hyperbranched polymerics

Peleshanko¹,

Tsukruk²

2011

J Polym Sci B Polym Phys

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A condensed overview discusses the existing grafting approaches and the surface behavior of various hyperbranched polymers. We focus on the recent strategies and corresponding characterization of the resulting surface morphologies and structures with a number of relevant recent results from the authors' own research and existing literature. Some results discussed here are important for prospective applications of hyperbranched polymers in biomedical fields, for resistive coatings, tough blends, and reinforced nanocomposites are briefly summarized as well. V C 2011 Wiley Periodicals, Inc. J Polym Sci Part B: Polym Phys 50: 83-100, 2012 KEYWORDS: adsorption; hyperbranched; LB films; structural characterization; surfaces INTRODUCTION This publication presents a condensed overview of the existing grafting approaches as applied to hyperbranched polymers and discusses the surface morphologies of these polymers and corresponding composites and coatings. We mostly focus on the recent strategies and corresponding characterization of the resulting surface morphologies and structures developed in the authors' group with some relevant examples from current literature. A number of relevant results are also included here especially in relation to recent developments related to novel synthetic approaches of hyperbranched molecules as well as emerging applications of hyperbranched polymers and coatings for biomedical purposes, as resistive coatings, tough blends, and reinforced nanocomposites. Some comprehensive recent reviews on highly branched polymers, which are focused mainly on synthetic aspects can be found in literature.

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Section: Hyperbranched Polymers For Biomedical Applicationsmentioning

confidence: 99%

Assembling hyperbranched polymerics

Peleshanko¹,

Tsukruk²

2011

J Polym Sci B Polym Phys

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“…16, 17 Moreover, hydrogels have also been reported that combine HPG and PEG macromonomers in aqueous droplets, allowing for encapsulation of living yeast cells into the resultant microgels. 18 As a result, we hypothesize that the high functionality and compact structure of HPGs allows additional control over PEGDA hydrogel mechanical properties, without impacting hydrogel pore structure and diffusion properties.…”

Section: Introductionmentioning

confidence: 97%

Patterning Three-Dimensional Hydrogel Microenvironments Using Hyperbranched Polyglycerols for Independent Control of Mesh Size and Stiffness

et al. 2017

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The extracellular matrix (ECM) is an environment rich with structural, mechanical, and molecular signals that can impact cell biology. Traditional approaches in hydrogel biomaterial design often rely on modifying the concentration of cross-linking groups to adjust mechanical properties. However, this strategy provides limited capacity to control additional important parameters in 3D cell culture such as microstructure and molecular diffusivity. Here we describe the use of multifunctional hyperbranched polyglycerols (HPGs) to manipulate the mechanical properties of polyethylene glycol (PEG) hydrogels while not altering biomolecule diffusion. This strategy also provides the ability to separately regulate spatial and temporal distribution of biomolecules tethered within the hydrogel. The functionalized HPGs used here can also react through a copper-free click chemistry, allowing for the encapsulation of cells and covalently tethered biomolecules within the hydrogel. Because of the hyperbranched architecture and unique properties of HPGs, their addition into PEG hydrogels affords opportunities to locally alter hydrogel crosslinking density with minimal effects on global network architecture. Additionally, photocoupling chemistry allows micropatterning of bioactive cues within the three-dimensional gel structure. This approach therefore enables us to tailor mechanical and diffusive properties independently while further allowing for local modulation of biomolecular cues to create increasingly complex cell culture microenvironments.

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“…27,28 It has been known that direct exposure to initiators and prolonged irradiation during polymerization led to poor cytocompatibility. 27,28 Hence, it is vital for an injectable hydrogel system to cross-link under mild conditions.…”

Section: Introductionmentioning

confidence: 99%

An injectable hydrogel formed by in situ cross-linking of glycol chitosan and multi-benzaldehyde functionalized PEG analogues for cartilage tissue engineering

et al. 2015

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In this study, a muti-benzaldehyde functionalized poly(ethylene glycol) analogue, poly(ethylene oxide-co-glycidol)-CHO (poly(EO-co-Gly)-CHO), was designed and synthesized for the first time, and applied as a cross-linker to develop an injectable hydrogel system. Simply mixing two aqueous precursor solutions of glycol chitosan (GC) and poly(EO-co-Gly)-CHO led to the in situ formation of chemically cross-linked hydrogels under physiological conditions. The cross-linking was attributed to a Schiff's base reaction between amino groups of GC and aldehyde groups of poly(EO-co-Gly)-CHO. The gelation time, water uptake, mechanical properties and network morphology of the GC/poly(EO-co-Gly) hydrogels were well modulated by varying the concentration of poly(EO-co-Gly)-CHO. Degradation of the in situ formed hydrogels was confirmed both in vitro and in vivo. The integrity of the GC/poly(EO-co-Gly) hydrogels was maintained for up to 12 weeks subcutaneously in ICR mice. The feasibility of encapsulating chondrocytes in the GC/poly(EO-co-Gly) hydrogels was assessed. Live/Dead staining assay demonstrated that the chondrocytes were highly viable in the hydrogels, and no dedifferentiation of chondrocytes was observed after 2 weeks of in vitro culture. Cell counting kit-8 assay gave evidence of the remarkably sustained proliferation of the encapsulated chondrocytes. Maintenance of the chondrocyte phenotype was also confirmed with an examination of characteristic gene expression. These features suggest that GC/poly(EO-co-Gly) hydrogels hold potential as an artificial extracellular matrix for cartilage tissue engineering.

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Hyperbranched polyglycerols on the nanometer and micrometer scale

Cited by 102 publications

References 38 publications

Assembling hyperbranched polymerics

Assembling hyperbranched polymerics

Patterning Three-Dimensional Hydrogel Microenvironments Using Hyperbranched Polyglycerols for Independent Control of Mesh Size and Stiffness

An injectable hydrogel formed by in situ cross-linking of glycol chitosan and multi-benzaldehyde functionalized PEG analogues for cartilage tissue engineering

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