Hyperbranched polyglycerol-grafted titanium oxide nanoparticles: synthesis, derivatization, characterization, size separation, and toxicology

Qin, Hongmei; Maruyama, Kyouhei; Amano, Tsukuru; Murakami, Takashi; Komatsu, Naoki

doi:10.1088/2053-1591/3/10/105049

Cited by 17 publications

(12 citation statements)

References 33 publications

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“…In addition to the more hydrophilic nature of PG, the hyperbranched structure of PG through the “grafting from” process is another advantage over PEG due to dense surface coverage of the NP, which should improve the protein resistivity. Furthermore, biocompatibility, facility, applicability, and extensibility make PG more attractive; that is, PG functionalization is a simple one-pot process which can be applied to not only various NPs, but also porous NPs, one-dimensional nanotubes, and two-dimensional nanosheets. ,,, A number of hydroxyl groups on PG can be derivatized to add more functions such as targeted cancer imaging and drug delivery by conjugating with fluorescence dye (Cy7), active targeting moiety (RGD motif), and anticancer drugs (cisplatin and doxorubicin). , The outstanding anti-biofouling property of PG presented here may bring about a paradigm shift in nanomedicine to change the design of the NPs for in vivo theranostic applications and even the coating of the film for ex vivo biosensor applications.…”

Section: Discussionmentioning

confidence: 99%

“…Polyglycerol (PG), which consists of a large number of hydroxyl groups on the PEG backbone (Scheme ), is expected to have a higher potential to prevent protein adsorption due to its nonionic and more hydrophilic nature. ,, Actually, PG on a gold substrate was reported to exhibit similar or even higher protein resistance than PEG to the proteins such as fibrinogen, lysozyme, albumin, and pepsin. − On the other hand, we established the methodology to functionalize NP surfaces more facilely and densely with PG − and demonstrated their very low nonspecific cellular uptake. ,, In order to fully explore the potential of PG as an alternative to PEG, we quantitatively compared the PG- and PEG-functionalized NPs in protein corona formation and macrophage uptake to reveal clearly that PG resists the protein adsorption and the macrophage uptake much more efficiently than does PEG. In particular, only a little protein is adsorbed on the NP surface coated with PG at 30 wt %, leading to almost no macrophage uptake.…”

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confidence: 99%

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Polyglycerol Grafting Shields Nanoparticles from Protein Corona Formation to Avoid Macrophage Uptake

Zou¹,

Ito²,

Yoshino

et al. 2020

ACS Nano

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113

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Upon contact with biofluids, proteins are quickly adsorbed onto the nanoparticle (NP) surface to form a protein corona, which initiates the opsonization and facilitates the rapid clearance of the NP by macrophage uptake. Although polyethylene glycol (PEG) functionalization has been the standard approach to evade macrophage uptake by reducing protein adsorption, it cannot fully eliminate nonspecific uptake. Herein, polyglycerol (PG) grafting is demonstrated as a better alternative to PEG. NPs of various size and material were grafted with PG and PEG at 30, 20, and 10 wt % contents by controlling the reaction conditions, and the resulting NP-PG and NP-PEG were characterized qualitatively by IR spectroscopy and quantitatively by thermogravimetric analysis. Their resistivity to adsorption of the proteins in fetal bovine serum and human plasma were compared by polyacrylamide gel electrophoresis, bicinchoninic acid assay, and liquid chromatography-tandem mass spectrometry, giving a consistent conclusion that PG shields protein adsorption more efficiently than does PEG. The macrophage uptake was assayed by transmission electron microscopy and by extinction spectroscopy or inductively coupled plasma mass spectrometry, revealing that PG avoids macrophage uptake more efficiently than does PEG. In particular, a NP coated with PG at 30 wt % (NP-PG-h) prevents corona formation almost completely, regardless of NP size and core material, leading to the complete evasion of macrophage uptake. Our findings demonstrate that PG grafting is a promising strategy in nanomedicine to improve anti-biofouling property and stealth efficiency in nanoformulations.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Polyglycerol Grafting Shields Nanoparticles from Protein Corona Formation to Avoid Macrophage Uptake

Zou¹,

Ito²,

Yoshino

et al. 2020

ACS Nano

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113

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“…The surfactant gave large dispersibility to nanodiamond, which is not less than 20, 16, and 6 mg / mL in pure water, phosphate buffered saline, and methanol, respectively [45]. The PG functionalization was found to be applicable to various nanoparticles such as iron oxide [46], titanium oxide [47], and zinc oxide [48] to impart good aqueous dispersibility. In addition, further derivatization of the PG layer enables nanodiamond and iron oxide nanoparticle to be employed as a drug carrier and an imaging probe [49][50][51][52][53].…”

Section: Polyglycerol For Solubilization Of Nanodiamondmentioning

confidence: 98%

Various surfactants for 0 – 3 dimensional nanocarbons: Separation, exfoliation and solubilization

Komatsu

2019

Adv. Mater. Lett.

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Nanocarbons are carbon allotropes with nanometer scale and comprised mainly of 0-3 dimensional (0D-3D) forms; fullerenes (0D), carbon nanotubes (1D), graphene (2D), and nanodiamond (3D). In our group, various surfactants with appropriate size and shape have been developed for the nanocarbons. In order to separate the fullerenes, bowl-shaped surfactants were designed and synthesized to accommodate the 0D spherical nanocarbon, giving C70 selectively as precipitates. On the other hand, gable-and bracket-shaped surfactants formed stable complexes with 1D tubular nanocarbons, dispersing carbon nanotubes with specific diameter, handedness and/or metallicity selectively in solution phase. The flat surfactants worked as an exfoliant and dispersant for graphite in both wet and dry processes; sonication and ball milling, respectively. They gave graphene composites with high concentrations and yield in aqueous solution and low-boiling point organic solvents. The hyper-branched polymer named polyglycerol coated the nanodiamond surface covalently through ring-opening polymerization of glycidol. The chemisorped polymer gave large hydrophilicity to the nanodiamond, dispersing it stably in aqueous solutions such as water and phosphate buffer as well as polar organic solvents such as methanol.

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“…The systems had high positive zeta potentials demonstrating their potential ability for gene delivery. 102 Later, the authors used a similar process for the functionalization of mesoporous bioactive glass (MBG) 103 and TiO 2 nanoparticles 104 instead of ND. The synthesized nanosystems indicated high efficiency for gene delivery and biomedical applications, respectively.…”

Section: Nanodiamondsmentioning

confidence: 99%

Modification of carbon-based nanomaterials by polyglycerol: recent advances and applications

Rafiee

Omidi

2022

RSC Adv.

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Hyperbranched polyglycerol-grafted titanium oxide nanoparticles: synthesis, derivatization, characterization, size separation, and toxicology

Cited by 17 publications

References 33 publications

Polyglycerol Grafting Shields Nanoparticles from Protein Corona Formation to Avoid Macrophage Uptake

Polyglycerol Grafting Shields Nanoparticles from Protein Corona Formation to Avoid Macrophage Uptake

Various surfactants for 0 – 3 dimensional nanocarbons: Separation, exfoliation and solubilization

Modification of carbon-based nanomaterials by polyglycerol: recent advances and applications

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