Hyperbaric oxygenation

Chew, H. E. R.; Hanson, Gillian C.; Slack, W. K.

doi:10.1016/s0007-0971(69)80010-0

Cited by 5 publications

(1 citation statement)

References 74 publications

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“…Nowadays, hyperbaric gases (especially oxygen) are also used in the postoperative or regenerative medicine, i.e. in the treatment of diving accidents and in the hyperbaric oxygen therapy (Chew et al, 1969;Lillehei et al, 1964;Mutschler and Muth, 2001).…”

Section: Introductionmentioning

confidence: 99%

The effect of hyperbaric air on the electric activity of neuronal in vitro networks

Stubbe

Nissen

Schroeder

et al. 2015

Biosensors and Bioelectronics

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Breathing hyperbaric air or gas mixtures, for example during diving or when working underwater is known to alter the electrophysiological behavior of neuronal cells, which may lead to restricted cognition. During the last few decades, only very few studies into hyperbaric effects have been published, especially for the most relevant pressure range of up to 10 bar. We designed a pressurized measuring chamber to record pressure effects on the electrical activity of neuronal networks formed by primary cells of the frontal cortex of NMRI mice. Electrical activity was recorded with multi-electrode arrays (MEAs) of glass neuro chips while subjected to a step-by-step pressure increase from atmospheric pressure (1 bar) to 2 and 4 bar, followed by a decompression to 1 bar, in order to record recovery effects. The effects of pressure on the total spike rates (TSRs), which were averaged from at least 45 chips, were detected in two cell culture media with different compositions. In a DMEM medium with 6% horse serum, the TSR was increased by 19% after a pressure increase to 2 bar and remained stable at 4 bar. In NMEM medium with 2% B27, the TSR was not altered by a pressure increase to 2 bar but increased by 9% at 4 bar. After decompression to 1 bar, the activities decreased to 76% and 101% of their respective control levels in the two media. MEA recordings from neuronal networks in miniaturized hyperbaric measuring chambers provide new access for exploring the neuronal effects of hyperbaric breathing gases.

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Section: Introductionmentioning

confidence: 99%