Hyperbaric Oxygen Therapy Alleviates Oxidative Stress and Tissue Injury in Renal Ischemia/Reperfusion Injury in Rats

H, Ilhan; Eroğlu, Murat; İnal, Volkan; Eyi, Yusuf Emrah; Arziman, Ibrahim; Yıldırım, Ali Osman; Tansel, Ali; Uzun, Günalp; Yamanel, Levent

doi:10.3109/0886022x.2012.723776

Cited by 13 publications

(12 citation statements)

References 17 publications

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“…Another study found that HBOT reduced neutrophil infiltration after ischemia reperfusion injury and also limited lipid peroxidation by increasing antioxidant enzymes. Although the exact mechanisms of HBOT renal benefits were not clear, the authors speculated that the beneficial effects of HBOT were mediated via inhibiting neutrophil infiltration [19]. …”

Section: Discussionmentioning

confidence: 99%

The Effect of Hyperbaric Oxygen Therapy on Kidneys in a Rat Model

Berkovitch

Tsadik

Kozer

et al. 2014

The Scientific World Journal

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Background. Hyperbaric oxygen therapy (HBOT) is used for treating various medical conditions. As far as known yet, HBOT is safe with few major side effects that are easy to avoid using a proper protocol. Renal tubular damage was observed in rats exposed to HBOT in a preliminary study conducted in our institution. Aim. We aim to assess whether HBOT causes renal damage and, if so, whether this is dose dependent. Methods. Thirty-one rats were randomly assigned to three groups. The first group received 10-days HBOT, 100% oxygen at a pressure of 2 atmospheres absolute (2 ATA) for 60 minutes/day, the second received the same treatment for 5 days and the third served as the control. Rat weight, survival, renal function tests, and renal histopathology were analyzed. Results. There were no significant changes in renal function tests in the plasma (cystatin C, urea, creatinine, and electrolytes) between the groups. No significant differences were observed in weight gain or renal histopathological evaluation between all groups. Conclusion. HBOT in this protocol does not cause renal impairment in a rat model, which reinforces the assumption that HBOT is safe in healthy rats, regarding renal function. Further research should be focused on the effect/safety of HBOT on nonhealthy kidneys.

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Section: Discussionmentioning

confidence: 99%

The Effect of Hyperbaric Oxygen Therapy on Kidneys in a Rat Model

Berkovitch

Tsadik

Kozer

et al. 2014

The Scientific World Journal

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“…However, HBO has been shown to decrease lipid peroxidation in a number of studies (8,18,22). A study by Ilhan et al determined that HBO therapy attenuated MDA levels by increasing SOD and GPx activities in rats with renal ischemia reperfusion (6). In recent years, diverse oxidant species can be measured totally and lipid peroxidation activities have been analyzed by evaluating TOS (23).…”

Section: A B Cmentioning

confidence: 99%

“…HBO therapy involves the intermittent inhalation of 100% oxygen at a pressure higher than 1 standard atmospheric pressure (6). This therapy enhances the dissolved oxygen concentration in arterial blood and restores the diffusion of oxygen into poorly perfused tissues (6,7). Animal studies have shown that HBO therapy attenuates ischemia reperfusion-induced tissue injury by abating oxidative stress, suppressing inflammation, increasing nitric oxide formation and preventing apoptosis (7)(8)(9).…”

Section: Introductionmentioning

confidence: 99%

Attenuation of partial unilateral ureteral obstruction-induced renal damage with hyperbaric oxygen therapy in a rat model

Sancak¹,

Tan²,

Türkön³

et al. 2017

European Urology Supplements

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ARTICLE INFO ______________________________________________________________ ______________________Objective: The objective of the present study was to evaluate the effectiveness of HBO therapy on biochemical parameters, renal morphology and renal scintigraphy in rats undergoing chronic unilateral partial ureteral obstruction (UPUO). Material and methods: Thirty-five rats were divided into five equal groups: Control group; Sham group; HBO group; UPUO group and UPUO/HBO group. The effects of HBO therapy were examined using biochemical parameters and histopathological changes. After calculating the score for each histopathological change, the total histopathological score was obtained by adding all the scores. In addition, dynamic renal scintigraphy findings were evaluated. Results: Serum parameters indicating inflammation, serum tumor necrosis factoralpha, ischemia modified-albumin, IMA/albumin ratio and Pentraxin-3 levels, were observed to be high in the UPUO group and low in the UPUO/HBO treatment group. Similarly, in the treatment group, the reduction in malondialdehyde, total oxidant status and oxidative stress index levels and increase in total antioxidant capacity values were observed to be statistically significant compared to the UPUO group (p<0.001, p=0.007, p<0.001, p=0.001, respectively). The total score and apoptosis index significantly decreased after administration of HBO treatment. Dynamic 99mTc-MAG3 renal scintigraphy also showed convincing evidence regarding the protective nature of HBO against kidney injury. In the UPUO/HBO therapy group, the percentage contribution of each operated kidney increased significantly compared to the UPUO group (41.73% versus 32.72%). Conclusion:The findings of this study indicate that HBO therapy had a reno-protective effect by reducing inflammation and oxidative stress, and preserving renal function after renal tissue damage due to induction of UPUO.

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“…14 On the other hand, experimental studies conducted by our group and others have shown that HBO therapy alleviates renal injury induced by chemotherapy, ischemia/ reperfusion (I/R) injury, and sepsis. 10,[15][16][17] Furthermore, Ayvaz et al reported that HBO therapy (2.5 ATA 90 min, for 2 days) had a protective effect in an experimental MARF model in rats. 9 Therefore, further studies to elucidate the potential protective role of HBO therapy in MARF are warranted.…”

Section: Introductionmentioning

confidence: 99%

The effect of hyperbaric oxygen therapy on rhabdomyolysis-induced myoglobinuric acute renal failure in rats

et al. 2016

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Myoglobinuric acute renal failure (MARF) may develop after severe muscle injury. Heme oxygenase-1 (HO-1), a stress-response protein, has been implicated as a protective agent against MARF. We hypothesized that hyperbaric oxygen therapy (HBOT) may alleviate MARF by inducing renal HO-1 expression. Wistar-Albino rats were randomly assigned into three groups: Control (n = 4), MARF (n = 8), MARF + HBO (n = 8). MARF was induced by intramuscular glycerol (50%, 8 mL/kg) injection. Saline (8 mL/kg) was injected into the hind limb of the animals in the control group. Animals in the MARF + HBO group received two sessions of HBO therapy (90 min at 2.5 atm) 2 and 18 h after glycerol injection. Serum and tissue samples were taken at 24 h. Serum urea and creatinine levels increased in the MARF and MARF + HBO groups confirming the development of MARF. But, serum urea and creatinine levels were similar in MARF and MARF + HBO groups. Oxidative stress parameters were similar among all groups. Histological renal injury score was similar in MARF and MARF + HBO groups. HO-1 level, determined by immunohistochemistry, was significantly higher in MARF and MARF + HBO groups, compared to the control group. Although HO-1 level in MARF + HBO group was higher than MARF group, it was not statistically significant. We found that HBOT did not reduce renal injury in experimental MARF model. HBOT is used to reduce the muscle damage after crush injury, which may be accompanied by MARF. Therefore, more studies are needed to understand the effects of HBO treatment on renal functions after MARF.

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Hyperbaric Oxygen Therapy Alleviates Oxidative Stress and Tissue Injury in Renal Ischemia/Reperfusion Injury in Rats

Cited by 13 publications

References 17 publications

The Effect of Hyperbaric Oxygen Therapy on Kidneys in a Rat Model

The Effect of Hyperbaric Oxygen Therapy on Kidneys in a Rat Model

Attenuation of partial unilateral ureteral obstruction-induced renal damage with hyperbaric oxygen therapy in a rat model

The effect of hyperbaric oxygen therapy on rhabdomyolysis-induced myoglobinuric acute renal failure in rats

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