Hyperbaric Oxygen Preconditioning Upregulates Heme OxyGenase-1 and Anti-Apoptotic Bcl-2 Protein Expression in Spontaneously Hypertensive Rats with Induced Postischemic Acute Kidney Injury

Ostojic, Jelena Nesovic; Ivanov, Milan; Mihailović-Stanojević, Nevena; Karanovic, Danijela; Kovačević, Sanjin; Brkić, Predrag; Životić, Maja; Vajic, Una-Jovana; Jovović, Djurdjica; Jeremic, Rada; Ljubojević-Holzer, Senka; Miloradović, Zoran

doi:10.3390/ijms22031382

Cited by 13 publications

(11 citation statements)

References 51 publications

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“…Additionally, apocynin treatment and HBO preconditioning, single or combined, decreased the NGAL tubular expression, which should be considered as beneficial effect of these protocols on the course of induced postischemic AKI. These results are in a line with improved histological and morphological parameters of renal tissues obtained after APO treatment and HBO preconditioning in previous studies [ 47 , 52 ].…”

Section: Discussionsupporting

confidence: 91%

Immunohistochemical Analysis of 4-HNE, NGAL, and HO-1 Tissue Expression after Apocynin Treatment and HBO Preconditioning in Postischemic Acute Kidney Injury Induced in Spontaneously Hypertensive Rats

et al. 2021

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Oxidative stress has been considered as a central aggravating factor in the development of postischemic acute kidney injury (AKI). The aim of this study was to perform the immunohistochemical analysis of 4-hydroxynonenal (4-HNE), neutrophil gelatinase-associated lipocalin (NGAL), and heme oxygenase-1 (HO-1) tissue expression after apocynin (APO) treatment and hyperbaric oxygenation (HBO) preconditioning, applied as single or combined protocol, in postischemic acute kidney injury induced in spontaneously hypertensive rats (SHR). Twenty-four hours before AKI induction, HBO preconditioning was carried out by exposing to pure oxygen (2.026 bar) twice a day, for 60 min in two consecutive days. Acute kidney injury was induced by removal of the right kidney while the left renal artery was occluded for 45 min by atraumatic clamp. Apocynin was applied in a dose of 40 mg/kg body weight, intravenously, 5 min before reperfusion. We showed increased 4-HNE renal expression in postischemic AKI compared to Sham-operated (SHAM) group. Apocynin treatment, with or without HBO preconditioning, improved creatinine and phosphate clearances, in postischemic AKI. This improvement in renal function was accompanied with decreased 4-HNE, while HO-1 kidney expression restored close to the control group level. NGAL renal expression was also decreased after apocynin treatment, and HBO preconditioning, with or without APO treatment. Considering our results, we can say that 4-HNE tissue expression can be used as a marker of oxidative stress in postischemic AKI. On the other hand, apocynin treatment and HBO preconditioning reduced oxidative damage, and this protective effect might be expected even in experimental hypertensive condition.

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Section: Discussionsupporting

confidence: 91%

Immunohistochemical Analysis of 4-HNE, NGAL, and HO-1 Tissue Expression after Apocynin Treatment and HBO Preconditioning in Postischemic Acute Kidney Injury Induced in Spontaneously Hypertensive Rats

et al. 2021

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“…Hmox1 was induced by various stimuli that increase oxidative stress and was a homeostatic enzyme that is upregulated in response to stress [34]. It has been shown that increased expression of Hmox1 was one of the main protective mechanisms after post-ischemic acute kidney injury (AKI) in Wistar rats [35]. The antioxidant and protective role of Hmox1 in myocardial IRI has been con rmed [36].…”

Section: Discussionmentioning

confidence: 99%

Exploring biomarkers of intestinal ischemia reperfusion injury based on bioinformatics

chen,

Xu,

et al. 2023

Preprint

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Background: Intestinal ischemia reperfusion injury (IIRI) is relevant to crucial clinical manifestations. Hence, it was vital to screen the important biomarkers for the diagnosis and treatment of IIRI. Methods: The transcript sequence data was yielded to screen out differentially expressed genes (DEGs) in IIRI. WGCNA was conducted to select key module genes related to IIRI vs normal groups. Venn Diagram was utilized to identify candidate genes. Further, PPI network of candidate genes was established to show the interaction relationship. LASSO and SVM-RFE analysis were implemented to screen biomarker of IRI. We finally performed immune infiltration and functional enrichment analysis based on biomarkers. Finally, the vital biomarkers ofin IIRI were verified by quantitative PCRand immunohistochemical. Results: We identified 2919 DEGs and 1040 key module genes. Then, 483 candidate genes were obtained via venn diagram. Subsequently, 5 biomarkers (Ccl7, Cd14, Cxcl1, Hmox1 and Nfkbia) were identified via machine learning. Moreover, we found that these biomarkers was mainly enriched in the ‘reactome activation of NF -kappa-B in B cells’ pathway. We also found that 5 biomarkers were significantly positively correlated with actived DC, M2 Macrophage and Th2 cells, whereas all biomarkers was negatively correlated with immature DC, gammadelta T cells. For expression validation of the biomarkers, we observed higher expression of Ccl7,Cd14, Cxcl1, Hmox1 and Nfkbia in IIRI group in the sequencing data. we validated the expression in mouse tissue by RT-qPCR and immunohistochemical, which all tested and verified the conclusion of sequencing data analysis. Conclusion: Overall, we obtained 5 biomarkers (Ccl7, Cd14, Cxcl1, Hmox1 and Nfkbia), which laid a theoretical foundation for the mechanism and clinical research of IIRI.

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“…However, free radical scavenging by superoxide dismutase (SOD) protects against IRI (Huang et al, 2021;Ostojic et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

“…The production of antioxidant enzymes that scavenge free radicals in ischemic tissue is then impaired, thereby exacerbating the damage caused by these free radicals in the post ischemic reperfusion tissue. However, free radical scavenging by superoxide dismutase (SOD) protects against IRI ( Huang et al, 2021 ; Ostojic et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Pioglitazone Ameliorates Renal Ischemia-Reperfusion Injury via Inhibition of NF-κB Activation and Inflammation in Rats

Zou

Zhou

et al. 2021

Front. Physiol.

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Renal ischemia-reperfusion injury (IRI) is considered as a major cause of acute kidney injury. In this study, we investigated the role of the NF-κB signaling pathway and inflammation in the amelioration of renal IRI using pioglitazone. Sprague–Dawley (SD) rats were subjected to bilateral renal artery clamping for 45 min followed by perfusion restoration for establishing a simulated renal IRI model. At 24 h post-operatively, we assessed the serum levels of creatinine and urea nitrogen, expression levels of peroxisome proliferator-activated receptor gamma (PPAR-γ) and NF-κB-related (p-IKK-β and IκB-α) proteins, and mRNA expression levels of the inflammatory cytokines, including TNF-α and MCP-1, in the renal tissue of various study groups. The histopathological evaluation of renal tissue was also conducted. In rat renal tissue, pioglitazone treatment decreased the serum levels of post-renal IRI creatinine and urea nitrogen, as well as necrosis. Furthermore, it elevated the expression of PPAR-γ protein and decreased the expression of NF-κB-related proteins. Pioglitazone also decreased the mRNA expression of TNF-α and MCP-1 in the renal tissue. Thus, pioglitazone ameliorates renal IRI by inhibiting the NF-κB signaling pathway and inflammatory response in rats.

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Hyperbaric Oxygen Preconditioning Upregulates Heme OxyGenase-1 and Anti-Apoptotic Bcl-2 Protein Expression in Spontaneously Hypertensive Rats with Induced Postischemic Acute Kidney Injury

Cited by 13 publications

References 51 publications

Immunohistochemical Analysis of 4-HNE, NGAL, and HO-1 Tissue Expression after Apocynin Treatment and HBO Preconditioning in Postischemic Acute Kidney Injury Induced in Spontaneously Hypertensive Rats

Immunohistochemical Analysis of 4-HNE, NGAL, and HO-1 Tissue Expression after Apocynin Treatment and HBO Preconditioning in Postischemic Acute Kidney Injury Induced in Spontaneously Hypertensive Rats

Exploring biomarkers of intestinal ischemia reperfusion injury based on bioinformatics

Pioglitazone Ameliorates Renal Ischemia-Reperfusion Injury via Inhibition of NF-κB Activation and Inflammation in Rats

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