Hyperbaric oxygen affects endothelial progenitor cells proliferation in vitro

Benincasa, Julia C.; Filho, Luiz Henrique de Freitas; Carneiro, Giane Daniela; Sielski, Micheli S.; Giorgio, Selma; Werneck, Cláudio C.; Vicente, Cristina Pontes

doi:10.1002/cbin.11070

Cited by 16 publications

(9 citation statements)

References 29 publications

(34 reference statements)

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“…Benincasa et al. 19 found that hyperbaric oxygen therapy could reduce the tumor necrosis factor-α-mediated inflammatory responses of endothelial cells, thereby promoting vascular recovery following injury. Our study revealed that the experimental group showed a better prognosis than the control group; moreover, the experimental group showed a higher GCS score and lower NIHSS score after treatment than before treatment and performed better in both aspects relative to the control group.…”

Section: Discussionmentioning

confidence: 99%

RETRACTED: Hyperbaric oxygen for severe traumatic brain injury: a randomized trial

Zhong

Shan

et al. 2020

J Int Med Res

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Objective The present study aimed to explore the effects of hyperbaric oxygen therapy on the prognosis and neurological function of patients with severe traumatic brain injury. Methods A prospective study was carried out in 88 patients diagnosed with severe brain injury at our hospital and they were enrolled as research participants and randomly assigned to control and experimental groups (n = 44 per group) using a random number table method. Both groups underwent routine treatment. Patients in the experimental group were administered hyperbaric oxygen therapy approximately 1 week after admission when their vital signs had stabilized. Results No significant intergroup differences were observed in the Glasgow Coma Scale (GCS) and U.S. National Institutes of Health Stroke Scale (NIHSS) scores before treatment. However, after oxygen treatment, compared with the control group, the experimental group showed higher GCS and lower NIHSS scores. The GCS score at admission, tracheotomy status, and first hyperbaric oxygen therapy duration were independent prognostic factors in patients with severe traumatic brain injury. Conclusion Hyperbaric oxygen therapy may promote recovery of neurological function and improve the cognitive function and prognosis of patients with severe traumatic brain injury.

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Section: Discussionmentioning

confidence: 99%

RETRACTED: Hyperbaric oxygen for severe traumatic brain injury: a randomized trial

Zhong

Shan

et al. 2020

J Int Med Res

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“…The recruitment of these cells together with ISCs would represent an interesting broader effect of HBOT. Keeping in mind that HBOT has recently reported to increase the proliferation of progenitor cells in vitro (Benincasa et al;2019), our result would be the first in describing similar results in vivo in the intestinal niche. On the other hand, if, in addition, the target of acute HBOT are the so-called emergency ISCs, a population that has been described as quiescent (Muñoz et al;, this could mean that the treatment can promote their recruitment to ISCs.…”

Section: Discussionsupporting

confidence: 72%

Hyperbaric oxygen treatment increases intestinal stem cell proliferation through mTORc1 signaling in Mus musculus

casanova¹,

Peña-Villalobos²,

Arancibia-Altamirano³

et al. 2020

Preprint

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Background: Hyperbaric oxygen treatment (HBOT) has been used for more than a decade to treat diverse diseases like diabetic foot ulcers and ischemic injuries. More recently, HBOT has been reported to modulate proliferation of neural and intestinal stem cell populations, but the molecular mechanisms underlying these effects are not completely understood. Objective: In this study we aimed to determine HBOT stem cell modulation by evaluating in particular the role of the mTOR complex 1 (mTORc1), a key regulator of cell metabolism that modifies its activity depending on oxygen levels, as a potential mediator of HBOT in murine intestinal stem cells (ISCs). Methods: Mouse were exposed to 10 or 20 HBOT sessions and the proliferation of the ISCs were analyzed by immunofluorescence or immunohistochemistry using the specific ISCs marker, Olfm4. The regulation of HBOT and mTORc1 pathway was analyzed through S6K1 phosphorylation by western-blot and through the inhibition by rapamycin. Results: We discovered that acute HBOT can increase proliferation of ISCs in a synchronous fashion without affecting the animal’s oxidative metabolism. Noteworthy, the mTORc1 inhibitor rapamycin also increases the proliferation of ISCs. This effect has been attributed to its capacity to mimic a caloric restriction (CR). Interestingly, the combination of HBOT and rapamycin does not have a synergic effect. Nevertheless, HBOT can recover rapamycin induced mTORc1 inhibition, possibly acting through a competitive modulation on mTORC1. Conclusions: Collectively, our results suggest that HBOT proliferative effect on ISCs is modulated by mTORc1 signaling representing a promising new approach to treat intestinal conditions.

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“…So far, no studies evaluating the combinatorial effect of HBO and TNF-α on the metabolic activity of hADSCs have been published. The only work that examined the simultaneous effect of these two parameters on cell proliferation employed bone marrow-derived Endothelial Progenitor Cells, that were pre-exposed to the cytokine incubation before applying HBO [50]. Overall, considering the results of MTT assay and DNA content, the observed reduced cell proliferation following HBO treatment could be explained by induction of cell death or cell cycle arrest through the generation of ROS [51].…”

Section: Discussionmentioning

confidence: 99%

Hyperbaric Oxygen Therapy Improves the Osteogenic and Vasculogenic Properties of Mesenchymal Stem Cells in the Presence of Inflammation In Vitro

Gardin

Bosco

Ferroni

et al. 2020

IJMS

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Hyperbaric oxygen (HBO) therapy has been reported to be beneficial for treating many conditions of inflammation-associated bone loss. The aim of this work was to in vitro investigate the effect of HBO in the course of osteogenesis of human Mesenchymal Stem Cells (MSCs) grown in a simulated pro-inflammatory environment. Cells were cultured with osteogenic differentiation factors in the presence or not of the pro-inflammatory cytokine Tumor Necrosis Factor-α (TNF-α), and simultaneously exposed daily for 60 min, and up to 21 days, at 2,4 atmosphere absolute (ATA) and 100% O2. To elucidate osteogenic differentiation-dependent effects, cells were additionally pre-committed prior to treatments. Cell metabolic activity was evaluated by means of the MTT assay and DNA content quantification, whereas osteogenic and vasculogenic differentiation was assessed by quantification of extracellular calcium deposition and gene expression analysis. Metabolic activity and osteogenic properties of cells did not differ between HBO, high pressure (HB) alone, or high oxygen (HO) alone and control if cells were pre-differentiated to the osteogenic lineage. In contrast, when treatments started contextually to the osteogenic differentiation of the cells, a significant reduction in cell metabolic activity first, and in mineral deposition at later time points, were observed in the HBO-treated group. Interestingly, TNF-α supplementation determined a significant improvement in the osteogenic capacity of cells subjected to HBO, which was not observed in TNF-α-treated cells exposed to HB or HO alone. This study suggests that exposure of osteogenic-differentiating MSCs to HBO under in vitro simulated inflammatory conditions enhances differentiation towards the osteogenic phenotype, providing evidence of the potential application of HBO in all those processes requiring bone regeneration.

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Hyperbaric oxygen affects endothelial progenitor cells proliferation in vitro

Cited by 16 publications

References 29 publications

RETRACTED: Hyperbaric oxygen for severe traumatic brain injury: a randomized trial

RETRACTED: Hyperbaric oxygen for severe traumatic brain injury: a randomized trial

Hyperbaric oxygen treatment increases intestinal stem cell proliferation through mTORc1 signaling in Mus musculus

Hyperbaric Oxygen Therapy Improves the Osteogenic and Vasculogenic Properties of Mesenchymal Stem Cells in the Presence of Inflammation In Vitro

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