“Hyper‐bright” Near‐Infrared Emitting Fluorescent Organic Nanoparticles for Single Particle Tracking

Genin, Émilie; Gao, Zhaoshun; Varela, Juan A.; Daniel, Jonathan; Bsaibess, Talia; Gosse, Isabelle; Groc, Laurent; Cognet, Laurent; Blanchard‐Desce, Mireille

doi:10.1002/adma.201304602

Cited by 64 publications

(63 citation statements)

References 24 publications

(15 reference statements)

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“…Very recently, conjugated polymer dots (Pdots) are emerging as a new class of ultrabright fluorescent nanoprobes for biological imaging 22, 23, 24, 25, 26. They exhibit several important characteristics for in vitro and in vivo fluorescence studies, such as high brightness, fast emission rate, excellent photostability, nonblinking and nontoxic feature 23.…”

Section: Introductionmentioning

confidence: 99%

Thermally Activated Delayed Fluorescence Organic Dots (TADF Odots) for Time‐Resolved and Confocal Fluorescence Imaging in Living Cells and In Vivo

et al. 2017

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The fluorophores with long‐lived fluorescent emission are highly desirable for time‐resolved fluorescence imaging (TRFI) in monitoring target fluorescence. By embedding the aggregates of a thermally activated delayed fluorescence (TADF) dye, 2,3,5,6‐tetracarbazole‐4‐cyano‐pyridine (CPy), in distearoyl‐sn‐glycero‐3‐phosphoethanolamine‐poly(ethylene glycol) (DSPE‐PEG2000) matrix, CPy‐based organic dots (CPy‐Odots) with a long fluorescence lifetime of 9.3 μs (in water at ambient condition) and high brightness (with an absolute fluorescence quantum efficiency of 38.3%) are fabricated. CPy‐Odots are employed in time‐resolved and confocal fluorescence imaging in living Hela cells and in vivo. The green emission from the CPy‐Odots is readily differentiated from the cellular autofluorescence background because of their stronger emission intensities and longer lifetimes. Unlike other widely studied DSPE‐PEG2000 encapsulated Odots which are always distributed in cytoplasm, CPy‐Odots are located mainly in plasma membrane. In addition, the application of CPy‐Odots as a bright microangiography agent for TRFI in zebrafish is also demonstrated. Much broader application of CPy‐Odots is also prospected after further surface functionalization. Given its simplicity, high fluorescence intensity, and wide availability of TADF materials, the method can be extended to develop more excellent TADF Odots for accomplishing the challenges in future bioimaging applications.

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Section: Introductionmentioning

confidence: 99%

Thermally Activated Delayed Fluorescence Organic Dots (TADF Odots) for Time‐Resolved and Confocal Fluorescence Imaging in Living Cells and In Vivo

et al. 2017

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“…Semiconductor polymer dots (Pdots) exhibit tunable fluorescence, high brightness, and excellent photostability for optical imaging and sensing applications . The superior properties of Pdots have been widely demonstrated by cellular labeling, in vivo imaging, single‐particle tracking, and various bioassays . Recently, the extraordinary light absorption of semiconducting polymers was used as a basis for developing multifunctional probes for photoacoustic imaging and photothermal therapy .…”

Section: Introductionmentioning

confidence: 99%

Semiconducting Polymer Nanocavities: Porogenic Synthesis, Tunable Host–Guest Interactions, and Enhanced Drug/siRNA Delivery

Chen

Fang

Jin

et al. 2018

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Nanocavities composed of lipids and block polymers have demonstrated great potential in biomedical applications such as sensors, nanoreactors, and delivery vectors. However, it remains a great challenge to produce nanocavities from fluorescent semiconducting polymers owing to their hydrophobic rigid polymer backbones. Here, we describe a facile, yet general strategy that combines photocrosslinking with nanophase separation to fabricate multicolor, water-dispersible semiconducting polymer nanocavities (PNCs). A photocrosslinkable semiconducting polymer is blended with a porogen such as degradable macromolecule to form compact polymer dots (Pdots). After crosslinking the polymer and removing the porogen, this approach yields semiconducting polymer nanospheres with open cavities that are tunable in diameter. Both small molecules and macromolecules can be loaded in the nanocavities, where molecular size can be differentiated by the efficiency of the energy transfer from host polymer to guest molecules. An anticancer drug doxorubicin (Dox) is loaded into the nanocavities and the intracellular release is monitored in real time by the fluorescence signal. Finally, the efficient delivery of small interfering RNA (siRNA) to silence gene expression without affecting cell viability is demonstrated. The combined features of bright fluorescence, tunable cavity, and efficient drug/siRNA delivery makes these nanostructures promising for biomedical imaging and drug delivery.

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“…3,11 The absorption and fluorescence spectra of all the nanoparticles have been monitored during 11 weeks: while no significant variation of the absorption spectra is observed, a decrease of the emission spectra is detected. The relatively good colloidal stability appears to be further improved by polymer doping.…”

Section: Discussionmentioning

confidence: 99%

“…[1][2][3][4][5][6][7][8][9] The most commonly adopted way to prepare organic nanoparticles is the so-called reprecipitation method, 10 consisting in a solvent-exchange process: a concentrated solution of a hydrophobic compound, dissolved in an organic hydrophilic solvent, is rapidly introduced into a large amount of a non-solvent (generally water) under vigorous stirring. [1][2][3][4][5][6][7][8][9] The most commonly adopted way to prepare organic nanoparticles is the so-called reprecipitation method, 10 consisting in a solvent-exchange process: a concentrated solution of a hydrophobic compound, dissolved in an organic hydrophilic solvent, is rapidly introduced into a large amount of a non-solvent (generally water) under vigorous stirring.…”

Section: Introductionmentioning

confidence: 99%

Amplified two-photon brightness in organic multicomponent nanoparticles

et al. 2015

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In this paper we show how to easily obtain multicomponent fluorescent organic nanoparticles displaying an excitation energy-transfer cascade between the different molecular components. The use of an optically neutral polymer as dopant helps to increase the colloidal stability of the water suspensions and to slow down luminescence loss over time. In particular, core@shell@shell ternary nanoparticles are designed and investigated, providing a strongly increased luminescence in the red spectral region. The two-photon brightness of the ternary nanoparticles is greatly enhanced with respect to that of the single-component nanoparticles. In addition, these ternary nanoparticles can be two-photon excited over a broad spectral range (from 600 to 1200 nm) inside the biological transparency window. This property, together with the good colloidal stability in water suspension, suggests these fully organic multicomponent nanosystems to be very promising nanoprobes for bioimaging applications.

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“Hyper‐bright” Near‐Infrared Emitting Fluorescent Organic Nanoparticles for Single Particle Tracking

Cited by 64 publications

References 24 publications

Thermally Activated Delayed Fluorescence Organic Dots (TADF Odots) for Time‐Resolved and Confocal Fluorescence Imaging in Living Cells and In Vivo

Thermally Activated Delayed Fluorescence Organic Dots (TADF Odots) for Time‐Resolved and Confocal Fluorescence Imaging in Living Cells and In Vivo

Semiconducting Polymer Nanocavities: Porogenic Synthesis, Tunable Host–Guest Interactions, and Enhanced Drug/siRNA Delivery

Amplified two-photon brightness in organic multicomponent nanoparticles

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