Hyper and hypothyroidism change the expression and diurnal variation of thyroid hormone receptor isoforms in rat liver without major changes in their zonal distribution

Zandieh-Doulabi, Behrouz; Schiphorst, M. Platvoet-Ter; Kalsbeek, Andries; Wiersinga, W. M.; Bakker, O.

doi:10.1016/j.mce.2004.01.008

Cited by 16 publications

(13 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our results could indicate that in vivo depletion of TH might up-regulate TR expression in adult rat epididymis as was informed for other tissues as brain and liver (Zandieh- Doulabi et al 2004;Constantinou et al 2005). The diVerences in the levels of TR expression under thyroidectomy compared with that observed in the control model ascertain a speciWc function for TH on this organ.…”

Section: Discussionsupporting

confidence: 59%

Thyroid hormone receptor α1–β1 expression in epididymal epithelium from euthyroid and hypothyroid rats

Paul

Mukdsi

Pellizas

et al. 2008

Histochem Cell Biol

View full text Add to dashboard Cite

The objectives of the present work were to assess whether epithelial cells from the different segments of epididymis express TR alpha 1-beta 1 isoforms, to depict its subcellular immunolocalization and to evaluate changes in their expression in rats experimentally submitted to a hypothyroid state by injection of 131I. In euthyroid and hypothyroid groups, TR protein was expressed in epididymal epithelial cells, mainly in the cytoplasmic compartment while only a few one showed a staining in the nucleus as well. A similar TR immunostaining pattern was detected in the different segments of the epididymis. In hypothyroid rats, the number of TR-immunoreactive epithelial cells as well as the intensity of the cytoplasmic staining significantly increased in all sections analyzed. In consonance to the immunocytochemical analysis, the expression of TR alpha 1-beta 1 isoforms, assessed by Western blot revealed significantly higher levels of TR in cytosol compared to the nuclear fractions. Furthermore, TR expression of both alpha 1 and beta 1 isoforms and their mRNA levels were increased by the hypothyroid state. The immuno-electron-microscopy showed specific reaction for TR in principal cells associated with eucromatin, cytosolic matrix and mitochondria. The differences in expression levels assessed in control and thyroidectomized rats ascertain a specific function of TH on this organ.

show abstract

Section: Discussionsupporting

confidence: 59%

Thyroid hormone receptor α1–β1 expression in epididymal epithelium from euthyroid and hypothyroid rats

Paul

Mukdsi

Pellizas

et al. 2008

Histochem Cell Biol

View full text Add to dashboard Cite

show abstract

“…In the context of hypothyroidism, the hypocholesterolemic effect of n-3 PUFA is still observed, while the hypotriglyceridemic effect of these fatty acids was lost. Hypothyroidism was not associated with changes in liver TRb1 or TRa expression, in disagreement with some studies (Zandieh-Doulabi et al 2004), and in agreement with others (Hodin et al 1990, Strait et al 1990). On the other hand, hypothyroidism induced a marked increase in liver expression of PPARa.…”

Section: Discussionsupporting

confidence: 44%

Thyroid hormone contributes to the hypolipidemic effect of polyunsaturated fatty acids from fish oil: in vivo evidence for cross talking mechanisms

Souza¹,

Cordeiro²,

Oliveira³

et al. 2011

Journal of Endocrinology

View full text Add to dashboard Cite

n-3 polyunsaturated fatty acids (n-3 PUFA) from fish oil (FO) exert important lipid-lowering effects, an effect also ascribed to thyroid hormones (TH) and TH receptor b1 (TRb1)-specific agonists. n-3 PUFA effects are mediated by nuclear receptors, such as peroxisome proliferator-activated receptors (PPAR) and others. In this study, we investigated a role for TH signaling in n-3 PUFA effects. Euthyroid and hypothyroid adult rats (methimazole-treated for 5 weeks) received FO or soybean oil (control) by oral administration for 3 weeks. In euthyroid rats, FO treatment reduced serum triglycerides and cholesterol, diminished body fat, and increased protein content of the animals. In addition, FO-treated rats exhibited higher liver expression of TRb1 and mitochondrial a-glycerophosphate dehydrogenase (mGPD), at protein and mRNA levels, but no alteration of glutathione S-transferase or type 1 deiodinase. In hypothyroid condition, FO induced reduction in serum cholesterol and increase in body protein content, but lost the ability to reduce triglycerides and body fat, and to induce TRb1 and mGDP expression. FO did not change PPARa liver abundance regardless of thyroid state; however, hypothyroidism led to a marked increase in PPARa liver content but did not alter TRb1 or TRa expression. The data suggest that part of the effect of n-3 PUFA from FO on lipid metabolism is dependent on TH signaling in specific steps and together with the marked upregulation of PPARa in liver of hypothyroid rats suggest important in vivo consequences of the cross-talking between those fatty acids and TH pathways in liver metabolism.

show abstract

“…Thus, if a signal leads to a decrease in D1, the result will be a decrease in T 3 production that leads to a further decrease in D1 expression, initiating a negative vicious circle that can, however, be broken by the opposite regulation of D2 expression in other tissues. Moreover, it is known that D1 as well as TR expression are under diurnal rhythm control in relation to the animal's biological clock and feeding behavior (52). Here, by performing experiments at beginning of the light period and within a restricted time schedule, we have shown that, in both liver and kidney, D1 mRNA expressions and protein levels decrease progressively with age, with the liver D1 expression level showing the greater sensitivity to aging.…”

Section: Discussionmentioning

confidence: 68%

Age-related changes in renal and hepatic cellular mechanisms associated with variations in rat serum thyroid hormone levels

Silvestri

Lombardi²,

Lange³

et al. 2008

American Journal of Physiology-Endocrinology and Metabolism

View full text Add to dashboard Cite

Aging is associated with changes in thyroid gland physiology. Age-related changes in the contribution of peripheral tissues to thyroid hormone serum levels have yet to be systematically assessed. Here, we investigated age-related alterations in the contributions of the liver and kidney to thyroid hormone homeostasis using 6-, 12-, and 24-mo-old male Wistar rats. A significant and progressive decline in plasma thyroxine occurred with age, but triiodothyronine (T(3)) was decreased only at 24 mo. This was associated with an unchanged protein level of the thyroid hormone transporter monocarboxylate transporter 8 (MCT8) in the kidney and with a decreased MCT8 level in the liver at 24 mo. Hepatic type I deiodinase (D1) protein level and activity declined progressively with age. Renal D1 levels were decreased at both 12 and 24 mo but D1 activity was decreased only at 24 mo. In the liver, no changes occurred in thyroid hormone receptor (TR) TRalpha(1), whereas a progressive increase in TRbeta(1) occurred at both mRNA and total protein levels. In the kidney, both TRalpha(1) and TRbeta(1) mRNA and total protein levels were unchanged between 6 and 12 mo but increased at 24 mo. Interestingly, nuclear TRbeta1 levels were decreased in both liver and kidney at 12 and 24 mo, whereas nuclear TRalpha(1) levels were unchanged. Collectively, our data show differential age-related changes among hepatic and renal MCT8 and D1 and TR expressions, and they suggest that renal D1 activity is maintained with age to compensate for the decrease in hepatic T(3) production.

show abstract

Hyper and hypothyroidism change the expression and diurnal variation of thyroid hormone receptor isoforms in rat liver without major changes in their zonal distribution

Cited by 16 publications

References 24 publications

Thyroid hormone receptor α1–β1 expression in epididymal epithelium from euthyroid and hypothyroid rats

Thyroid hormone receptor α1–β1 expression in epididymal epithelium from euthyroid and hypothyroid rats

Thyroid hormone contributes to the hypolipidemic effect of polyunsaturated fatty acids from fish oil: in vivo evidence for cross talking mechanisms

Age-related changes in renal and hepatic cellular mechanisms associated with variations in rat serum thyroid hormone levels

Contact Info

Product

Resources

About