Hymenolepis diminuta: Intestinal goblet cell response to infection in male C57 mice

McKay, Derek M.; Halton, D W; McCaigue, M. D.; Johnston, C.F.; Fairweather, Ian; Shaw, Christopher

doi:10.1016/0014-4894(90)90003-u

Cited by 43 publications

(48 citation statements)

References 36 publications

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“…In other experiments, mice received CFA and 2 days later were infected with H diminuta. Other mice were infected with H diminuta and 28 days later received CFA with or without a simultaneous secondary infection or PBS as a control (by this time, immunologic memory against H diminuta has been generated) (16). Some mice were treated with indomethacin (250 g/ mouse) (17) or dexamethasone (25 g/mouse) (18,19) (both from Sigma) on days Ϫ1, 0, 1, and 2 after receiving CFA by subcutaneous injection, for comparison with mice infected with H diminuta.…”

Section: Methodsmentioning

confidence: 99%

Infection with an intestinal helminth parasite reduces Freund's complete adjuvant–induced monoarthritis in mice

Shi¹,

Wang²,

Prescott³

et al. 2011

Arthritis & Rheumatism

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Objective. Assessment of infection with helminth parasites in murine models of disease could identify antiinflammatory mechanisms that translate into treatments for arthritic disease. The aim of this study was to test the ability of infection with the tapeworm Hymenolepis diminuta to ameliorate Freund's complete adjuvant (CFA)-induced monoarthritis in mice.Methods. Mice received CFA with or without H diminuta, and knee swelling, pain, and measures of inflammation were assessed.Results. Injection of CFA resulted in rapid (within 24 hours) and sustained (lasting 20 days) knee swelling, a decreased pain threshold, increased blood flow to the knee, and increased production of tumor necrosis factor ␣ and interleukin-12p40 (IL-12p40). In mice that were infected with H diminuta 8 days prior to receiving CFA, the severity of arthritis was reduced as assessed by these indices of inflammation and infection 2 days after CFA injection and resulted in more rapid resolution of knee swelling. This antiarthritic effect required a viable infection and was dependent on adaptive immunity, because infection with H diminuta did not protect mice lacking T cells and B cells or the IL-4 receptor ␣ chain from CFA-induced inflammation. Interleukin-10 was of prime importance in the antiarthritic effect, because IL-10-knockout mice were not protected by infection, the antiarthritic effect was ablated by use of neutralizing IL-10 antibodies, and transfer of CD4؉ cells from infected wild-type mice but not IL-10-knockout mice significantly reduced CFAinduced knee swelling.Conclusion. In mice, the adaptive immune response to infection with H diminuta involves mobilization of IL-10, which has the concomitant advantage of dampening the innate immune responses that drive CFA-induced joint inflammation.

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Section: Methodsmentioning

confidence: 99%

Infection with an intestinal helminth parasite reduces Freund's complete adjuvant–induced monoarthritis in mice

Shi¹,

Wang²,

Prescott³

et al. 2011

Arthritis & Rheumatism

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“…In addition, H. diminuta is not autoinfective, and its life cycle requires cyclical passage through an intermediate insect host and a definitive host, the rat. Although H. diminuta will chronically parasitize the rat host, it is immunologically expelled from the mouse within 12 days of infection (15)(16)(17)). …”

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confidence: 99%

“…Mice received five infective H. diminuta cysticercoids by oral gavage in 100 l of 0.9% NaCl (15). Colitis was induced in lightly anesthetized mice by an intrarectal (ir.)…”

mentioning

confidence: 99%

“…In the treatment protocol, mice received DNBS 48 h before H. diminuta infection, followed by autopsy 8 days after H. diminuta infection. To check for worm infectivity, a segment of the jejunum at the ligament of Trietz was formalin fixed, embedded in paraffin, and stained with periodic acid-Schiff's reagent to identify goblet cells (15). In additional studies, mice were gavaged with five hot EtOH (70% for ϳ14 h)-killed cysticercoids, 1 mg/100 l PBS of adult H. diminuta protein Ag, or five excysted larvae (1% pepsin for 10 min, then placed in a 1% trypsin/1% sodium tauroglycocholate solution until the larvae emerged from the cyst).…”

mentioning

confidence: 99%

“…Male Sprague-Dawley rats (Harlan Animal Suppliers) were infected by oral gavage with 10 H. diminuta cysticercoids (15). In the chronic condition, colitis was induced 3 mo after infection by delivering 22 mg of DNBS in 100 l of EtOH into the colon via a 7.0-cm long catheter.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Neutralizing Anti-IL-10 Antibody Blocks the Protective Effect of Tapeworm Infection in a Murine Model of Chemically Induced Colitis

Hunter

Wang

Hirota

et al. 2005

The Journal of Immunology

151

212

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There is increasing evidence that parasitic helminth infection has the ability to ameliorate other disease conditions. In this study the ability of the rat tapeworm, Hymenolepis diminuta, to modulate dinitrobenzene sulfonic acid (DNBS)-induced colitis in mice is assessed. Mice receiving DNBS (3 mg intrarectally) developed colitis by 72 h after treatment. Mice infected 8 days before DNBS with five H. diminuta larvae were significantly protected from the colitis, as gauged by reduced clinical disease, histological damage scores, and myeloperoxidase levels. This anticolitic effect was dependent on a viable infection and helminth rejection, because no benefit was observed in mice given killed larvae or in infected STAT6 knockout mice or rats, neither of which eliminate H. diminuta. The anticolitic effect of H. diminuta was associated with increased colonic IL-10 mRNA and stimulated splenocytes from H. diminuta- plus DNBS-treated mice produced more IL-10 than splenocytes from DNBS-only treated mice. Coadministration of an anti-IL-10 Ab blocked the anticolitic effect of prophylactic H. diminuta infection. Also, mice infected 48 h after DNBS treatment showed an enhanced recovery response. Finally, using a model of OVA hypersensitivity, we found no evidence of concomitant H. diminuta infection enhancing enteric responsiveness to subsequent ex vivo OVA challenge. The data show that a viable infection of H. diminuta in a nonpermissive system exerts a profound anticolitic effect (both prophylactically and as a treatment) that is mediated at least in part via IL-10 and does not predispose to enhanced sensitivity to bystander proteins.

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Helminths for induction of remission in inflammatory bowel disease

Croft

Bager

Garg

et al. 2011

Cochrane Database of Systematic Reviews

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Hymenolepis diminuta: Intestinal goblet cell response to infection in male C57 mice

Cited by 43 publications

References 36 publications

Infection with an intestinal helminth parasite reduces Freund's complete adjuvant–induced monoarthritis in mice

Infection with an intestinal helminth parasite reduces Freund's complete adjuvant–induced monoarthritis in mice

Neutralizing Anti-IL-10 Antibody Blocks the Protective Effect of Tapeworm Infection in a Murine Model of Chemically Induced Colitis

Helminths for induction of remission in inflammatory bowel disease

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