Hydroxytyrosol induces antioxidant/detoxificant enzymes and Nrf2 translocation<i>via</i>extracellular regulated kinases and phosphatidylinositol-3-kinase/protein kinase B pathways in HepG2 cells

Martín, Maria Ángeles; Ramos, Sonia; Granado-Serrano, Ana Belén; Rodríguez-Ramiro, Ildefonso; Trujillo, Mariana; Bravo, Laura; Goya, Luis

doi:10.1002/mnfr.200900159

Cited by 117 publications

(97 citation statements)

References 39 publications

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“…The results obtained show the potential to prevent cell damage induced by tert-butyl hydroperoxide (t-BuOOH) and the ability to maintain unaltered cellular redox status, partially after 2 hours of pretreatment and almost completely after 20 hours. These results are in accordance with those obtained with hydroxytyrosol (Martín et al, 2010), but they also show the relevance of the role of the lipophilic character of the phenolic compounds on their antioxidant potential against cell damage: HT methyl and ethyl ethers are less effective than HT propyl and butyl ethers.…”

Section: Biological Activitysupporting

confidence: 89%

Biological Properties of Hydroxytyrosol and Its Derivatives

Fernandez‐Bolanos¹,

López²,

López-García³

et al. 2012

Olive Oil - Constituents, Quality, Health Properties and Bioconversions

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Section: Biological Activitysupporting

confidence: 89%

Biological Properties of Hydroxytyrosol and Its Derivatives

Fernandez‐Bolanos¹,

López²,

López-García³

et al. 2012

Olive Oil - Constituents, Quality, Health Properties and Bioconversions

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“…Multiple components of coffee have been shown to induce Nrf2 activation, including cafestol and kahweol (Higgins et al 2008), and cafestol is protective in the Drosophila PD models (Trinh et al 2010). A number of natural polyphenols, including hydroxytyrosol (Martin et al 2010), quercetin (Kimura et al 2009), and epigallocatechin-3-gallate (Na et al 2008), have also been shown to activate the Nrf2 pathway. These observations suggest that the skn-1/Nrf2 pathway has evolved to be broadly responsive to xenobiotic compounds, and that there may be multiple compounds in coffee extracts that contribute to activation of this pathway.…”

Section: Resultsmentioning

confidence: 99%

Genetic Mechanisms of Coffee Extract Protection in aCaenorhabditis elegansModel of β-Amyloid Peptide Toxicity

2010

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Epidemiological studies have reported that coffee and/or caffeine consumption may reduce Alzheimer's disease (AD) risk. We found that coffee extracts can similarly protect against b-amyloid peptide (Ab) toxicity in a transgenic Caenorhabditis elegans Alzheimer's disease model. The primary protective component(s) in this model is not caffeine, although caffeine by itself can show moderate protection. Coffee exposure did not decrease Ab transgene expression and did not need to be present during Ab induction to convey protection, suggesting that coffee exposure protection might act by activating a protective pathway. By screening the effects of coffee on a series of transgenic C. elegans stress reporter strains, we identified activation of the skn-1 (Nrf2 in mammals) transcription factor as a potential mechanism of coffee extract protection. Inactivation of skn-1 genetically or by RNAi strongly blocked the protective effects of coffee extract, indicating that activation of the skn-1 pathway was the primary mechanism of coffee protection. Coffee also protected against toxicity resulting from an aggregating form of green fluorescent protein (GFP) in a skn-1-dependent manner. These results suggest that the reported protective effects of coffee in multiple neurodegenerative diseases may result from a general activation of the Nrf2 phase II detoxification pathway.

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“…Further, in carbon tetrachloride-treated rat, an increase in the CAT activity was observed on olive oil treatment. Martin et al (2010) reported that HT increased the level of GPx in t-BOOH-treated HepG2 cells. SOD ( Figure 4C) and GR ( Figure 4D) activities were increased in TCDD-treated liver tissues compared with vehicle control.…”

Section: Restoration Of Tcdd-induced Antioxidant Changes By Oo Ht Amentioning

confidence: 98%

Olive oil and its phenolic compounds (hydroxytyrosol and tyrosol) ameliorated TCDD-induced heptotoxicity in rats via inhibition of oxidative stress and apoptosis

Kalaiselvan

Muniasamy

Archunan

et al. 2015

Pharmaceutical Biology

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Context: Naturally occurring polyphenols including olive oil (OO) and its constituents hydroxytyrosol (HT) and tyrosol (TY), consumed in the Mediterranean diet, have shown to treat various ailments due to their remarkable antioxidant properties. Objective: The present study investigates the hepatoprotective effects of OO and its phenolic compounds HT and TY against TCDD-induced hepatotoxicity in male Wistar rats. Materials and methods: TCDD was administered at a dose of 100 ng/kg p.o. for 20 d. Administration of OO (10 ml/kg; oral), HT (0.5 mg/kg; oral), and TY (30 mg/kg; i.p) was started 5 d prior to TCDD administration, and continued for 25 d with or without TCDD administration. At the end of the experiment (25 d), blood was taken for biochemical analyses and liver for the measurement of macromolecular damages, antioxidant status, expressions of CYP1A1, and apoptotic factors. Results: TCDD administration resulted in significant (p50.05) increase in the level of hepatic stress markers ALT (101.6 ± 3.07 IU/l), AST (295.0 ± 3.0 IU/l), and ALP (266.66 ± 3.7 IU/l). Also, biochemical analyses of liver reported elevation in nitrite and protein carbonyl content and depletion of NQO1 and HO. However, OO, HT, and TY restored the antioxidant status. Protein expressions by Western Blot technique showed an increase in the level of CYP1A1 and Bax and a decreased level of Bcl-2 on TCDD treatment, and vice versa on OO, HT, and TY treatment. Discussion and conclusion: Our work concludes that dietary supplementation of OO, HT, and TY could serve as a potential preventive drug for TCDD-induced hepatotoxicity.

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Hydroxytyrosol induces antioxidant/detoxificant enzymes and Nrf2 translocationviaextracellular regulated kinases and phosphatidylinositol-3-kinase/protein kinase B pathways in HepG2 cells

Cited by 117 publications

References 39 publications

Biological Properties of Hydroxytyrosol and Its Derivatives

Biological Properties of Hydroxytyrosol and Its Derivatives

Genetic Mechanisms of Coffee Extract Protection in aCaenorhabditis elegansModel of β-Amyloid Peptide Toxicity

Olive oil and its phenolic compounds (hydroxytyrosol and tyrosol) ameliorated TCDD-induced heptotoxicity in rats via inhibition of oxidative stress and apoptosis

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