Hydroxypropylmethylcellulose gel application delays Der p 1 diffusion in vitro

Abstract

Background: A special hydroxypropylmethylcellulose powder (Nasaleze®) has been used for the alleviation of nasal symptoms of allergic rhinitis since 1994. The efficacy of the product has been recently proven but the mechanism of action was still largely unknown. The aim of the study was to investigate the hypothesis that the gel formed after moisture absorption in the nose might act as mechanical barrier that prevents allergen diffusion towards the nasal epithelium. Methods: The diffusion of Der p 1 through HPMC and agar gels was measured in vitro after 15, 30, 60, 180 and 360 minutes using ELISA. Agar blocks were used to simulate the nasal mucosa. Control samples without gel layer were obtained. Results: The control samples with no applied gel barrier absorbed 72.2 % of the Der p 1 solution after 15 minutes and 100 % after 60 minutes. In comparison, the HPMC and agar gel layers both significantly delayed Der p 1 diffusion. After 15 minutes 0.76 % had diffused through the HPMC gel layer compared to 28.1 % which diffused through the agar layer. After 360 minutes, 14.1 % of the baseline Der p 1 crossed the HPMC gel layer while 100 % had diffused through the agar layer. Conclusions: HPMC gel significantly reduces Der p 1 diffusion in vitro compared to no barrier and an agar gel layer. This is likely to be due to the small mesh size of the polymer network of HPMC and could have important implications for a preventative treatment of allergic rhinitis.

<p>In vitro and in vivo Evaluation of the Efficacy and Safety of Powder Hydroxypropylmethylcellulose as Nasal Mucosal Barrier</p>

“…The first in vitro proof-of-concept study that measured the diffusion of Dermatophagoides pteronyssinus 1 allergen (Der-p-1, 24 kDa) through HPMC gel into agar blocks (1.5%, prepared with 0.9% saline solution) on microscope slides was done by Diethart et al 10 The barrier was prepared by mixing 50 mg of pHPMC with 1 mL physiological saline solution to form a 5% gel, 200 µL of which was applied to the open edge of the agar block (Figure 1). After putting a coverslip on top, 20 μL of Der p 1 solution (7.5 μg Derp-1 per 1 mL, Alk-Abello) were pipetted on the outer side of the pHPMC gel strip.…”

Benefits of Nasal Cellulose Powder Application Depend on the Type of Allergen Sensitization in Allergic Rhinitis

“…The in vitro study of Diethart et al 3 assessed the effect of NCP on the diffusion rate of Dermatophagoides pteronyssinus 1 ( Der p1 ) compared to agar gel and controls with no gel. In the absence of barrier (control) 72.2% of the Der p1 solution was absorbed after 15 minutes and 100% after 60 minutes.…”