Hydroxyproline in Urine and Tissues

Klein, L.; Faulkner, Willard R.; Pena, Armanda De La

doi:10.1016/b978-0-12-609106-9.50011-7

Cited by 31 publications

(11 citation statements)

References 36 publications

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“…The radioactivity in bone collagen was determined either on hydrolyzates or on hydroxyproline separated chromatographically [28] and expressed as total radioactivity per whole bone.…”

Section: Chemical Attd Isotopic Analyses Of Plasma and Bonementioning

confidence: 99%

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Effects of ethane-1-hydroxy-1,1-diphosphonate (EHDP) upon the kinetics of bone resorption and bone formation at the whole bone level in prelabelled chicks

Klein

Wong

1983

Calcif Tissue Int

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Chicks chronically prelabelled with 45Ca, 3H-tetracycline, and 3H-proline were used to measure the weekly effect of EHDP (5 mg P/kg for 28 days) on bone turnover at the whole bone level in vivo. Direct measurements were made of cortical bone resorption (loss of 3H-tetracycline and 3H-collagen from whole femur, blood-bone ratio of 45Ca), skeletal collagen formation and bone mineralization (collagen and calcium mass per whole femur, respectively). Chicks were sacrificed after 5, 14, 21 and 28 days of EHDP administration. By five days of treatment, EHDP caused a greater inhibition of bone mineralization (86%) than bone resorption (30-39%) without affecting skeletal collagen mass. The blood-bone ratio of 45Ca decreased 34%, which was similar in degree to the inhibition of 3H-tetracycline loss (30%) and 3H-collagen loss (39%). Almost complete inhibition of bone resorption and bone mineralization occurred by 14 days of treatment without effects on skeletal collagen mass. No additional effect was seen at 21 and 28 days of EHDP treatment. In chicks, EHDP inhibits almost completely bone mineralization (bone formation) and cortical bone resorption without affecting skeletal collagen mass.

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“…The radioactivity in bone collagen was determined either on hydrolyzates or on hydroxyproline separated chromatographically [28] and expressed as total radioactivity per whole bone.…”

Section: Chemical Attd Isotopic Analyses Of Plasma and Bonementioning

confidence: 99%

“…The organic residue remaining after acid extractions was hydrolyzed with 6 N HCl at 121~ and analyzed for hydroxyproline [28].…”

Section: Chemical Attd Isotopic Analyses Of Plasma and Bonementioning

confidence: 99%

Effects of ethane-1-hydroxy-1,1-diphosphonate (EHDP) upon the kinetics of bone resorption and bone formation at the whole bone level in prelabelled chicks

Klein

Wong

1983

Calcif Tissue Int

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“…After hydrolysis the samples were evaporated and dried in a vacuum desiccator over sodium hydroxide flakes. Citrate buffer (4 ml; O· 1 M; pH 2·9) was added to the dry residue and after steam-heating for 0·5 min the sample was filtered (Klein 1970). An aliquot was used for ninhydrinreactive material determination (Moore and Stein 1954).…”

Section: Hydroxyproline and Proline Contentmentioning

confidence: 99%

Effect of Intravenously Infused Dexamethasone on Collagen Metabolism in Skin of Merino Sheep

Leish¹,

Panaretto²

1979

Aust. Jnl. Of Bio. Sci.

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The effects of an 8-day intravenous infusion of dexamethasone (7·6 mg kg-o . 75 body weight) on collagen biosynthes's and wool growth in skin were examined in four Merino wethers.Plasma dexamethasone concentrations reached their peaks during the first 24 h infusion, which were followed by relatively stable levels (c. 1 x 10-7 M) for the next 4-5 days. Minor increases in plasma dexamethasone levels were recorded during the final 2 days of treatment. Dexamethasone concentrations quickly fell below the level of detection once infusion ceased.Marked decreases in the wet weight, thickness and protein content of skin were observed at the end of infusion. DNA content was reduced to 42·4±4·9 % s.e.m. during the first 2 days of treatment, but in the next 4 days of infusion gradually increased to 85·0±12·5% of controls. The level of collagen (expressed as hydroxyproline content of its acid hydrolysate) was elevated throughout the infusion and then gradually declined in 3 weeks to about 60 % of controls.The biosynthesis of collagen measured by the rate of [14C]hydroxyproline formation and the activity of proline, 2-oxoglutarate dioxygenase (EC 1.14.11.2, formerly prolyl hydroxylase) was reduced during the first half of treatment to a greater extent than the rate of [14C]proline incorporation into proteins.Wool growth was reduced by 80·4 ± 11 ·6% in the post-infusion period which allowed three sheep out of four to be defleeced.Inhibition of collagen biosynthesis in sheep skin was due initially to a decrease in the activity of proline, 2-oxoglutarate dioxygenase and later to the reduced rate of proline incorporation into proteins. It ,was also evident that changes in biosynthetic rate of collagen were not reflected in the total level of skin collagen. The extent of wool growth depression in individual animals paralleled the changes in DNA content and the extent of collagen biosynthesis inhibition.

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“…Because of the improved imino acid separation afforded by ion-exchange chromatography, the method of Klein (1970) was adopted. The initial calibration of each of four randomly-chosen columns was performed using a standard containing 0.1 ml (U)^'^C-hydroxyproline, ca.…”

Section: Skin Collagenmentioning

confidence: 99%

“…The unlabelled hydroxyproline was assayed by the Klein (1970) method. To 2 ml of sample or standard in a screw-capped Culture tube was added 1 ml of a freshly-mixed 1:1 solution of 0.015M CuSO^-15% NaOH.…”

Section: Skin Collagenmentioning

confidence: 99%

Effects of lead on collagen synthesis and renal function in the rat

Rodolfo-Sioson

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Hydroxyproline in Urine and Tissues

Cited by 31 publications

References 36 publications

Effects of ethane-1-hydroxy-1,1-diphosphonate (EHDP) upon the kinetics of bone resorption and bone formation at the whole bone level in prelabelled chicks

Effects of ethane-1-hydroxy-1,1-diphosphonate (EHDP) upon the kinetics of bone resorption and bone formation at the whole bone level in prelabelled chicks

Effect of Intravenously Infused Dexamethasone on Collagen Metabolism in Skin of Merino Sheep

Effects of lead on collagen synthesis and renal function in the rat

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