Hydroxyproline content of needle biopsies as an objective measure of liver fibrosis: Emphasis on sampling variability

Abstract

Although increases in collagen content (by hydroxyproline) approximately paralleled increases in fibrosis stage (by histology), there was considerable overlap and some discrepancies. Much work remains to be done to clarify the significance of the apparent huge variations in fibrosis within diseased livers. It is advisable for clinicians to interpret staging diagnoses by histological staging with some caution.

Prediction of fibrosis in hepatitis C patients: assessment using hydroxyproline and oxidative stress biomarkers

“…The data obtained were in accordance with Attallah et al [3] who correctly classified 82 % of CHC patients with severe liver fibrosis using fibrosis discriminate score (FDS). Also, Lee et al [24] and other previous reports [3], noted significant increment in the level of hydroxyproline as the fibrosis score increased in CHC patients.…”

“…The data obtained were correlated with the stage of liver fibrosis. This reflects the extensive degradation of newly synthesize collagen in the liver by collagenolytic enzymes during the early stages of fibrosis in patients with chronic liver diseases [27], and elevation in the level of hydroxyproline argue its importance as good marker for staging of hepatic fibrosis [24].…”

Dietary olive oil prevents carbon tetrachloride-induced hepatic fibrosis in mice

“…Aliquots (5 lL) of synthesized cDNA were added to 45 lL PCR mix containing 5 lL 109 PCR buffer, 1 lL each deoxynucleotide (1 mmol/L each), 0.5 lL sense and antisense primers (0.15 mmol/L), and 0.25 lL DNA polymerase (Gene Amp PCR kit, Perkin Elmer Cetus, Norwalk, CT). The reaction mixture was covered with a wax gem (Perkin Elmer Cetus), and amplification was initiated by 1 min denaturation at 94°C for 1 cycle, followed by multiple (28)(29)(30) cycles at 94°C for 30 s, 55°C for 30 s, and 72°C for 1 min using a GeneAmp PCR system 9800 DNA thermal cycler (Perkin Elmer Cetus). After the last cycle of amplification, samples were incubated for 7 min at 72°C.…”

“…The supernatant hydroxyproline concentration was determined as described elsewhere [29]. Briefly, 40 ll of the neutralized sample solution was removed to a 96-well ELISA plate and oxidized in each well with a solution containing 5 mL of 7% Chloramine T (Sigma, St Louis, MO, USA) and 20 mL of acetate/citrate buffer.…”

Magnetic resonance imaging of hepatic fibrosis: Emerging clinical applications

“…11 Although computerized image analysis protocols may offer the potential for quantitative analysis of liver fibrosis, 12 this method requires further study to provide standardized approaches to ensure reliability. 13 Ultimately, the method of percutaneous liver biopsy is an invasive procedure with poor acceptance by patients. The associated morbidity from this technique is estimated at 3% with a mortality rate of 0.03%.…”