Hydroxynitrile lyase-catalyzed addition of HCN to 2-substituted cyclopentanones

Immobilized Hydroxynitrile Lyases for Enantioselective Synthesis of Cyanohydrins: Sol‐Gels and Cross‐Linked Enzyme Aggregates

“…Here, we compare different forms of immobilized HNLs in diisopropyl ether (DIPE), a solvent used industrially for an HbHNLcatalyzed reaction. [5] In order to improve the HNLs robustness, immobilization onto supports such as celite, [10] cellulose [10,[12][13][14] or nitrocellulose [13,14] has been studied. Our attention focuses on immobilization strategies where the HNL can be recycled efficiently.…”

Evaluation of Several Routes to Advanced Pregabalin Intermediates: Synthesis and Enantioselective Enzymatic Reduction Using Ene-Reductases

“…The addition of sodium cyanide to β-keto ester 9 was evaluated (Scheme ) . Unfortunately, cyanohydrin 11 could not be obtained in significant yield via direct addition under all conditions attempted.…”

“…The addition of sodium cyanide to β-keto ester 9 was evaluated (Scheme 5). 7 Unfortunately, cyanohydrin 11 could not be obtained in significant yield via direct addition under all conditions attempted. It is worth mentioning that the reaction with trimethylsilyl cyanide 8 was not attempted because of the high cost of the reagent.…”

“…Ethyl 5-Methyl-3-cyano-2-hexenoate (7). The crude ∼4:1 mixture of cyanohydrin 11 and unreacted β-keto ester 9, (0.30 g, 1.50 mmol) was stirred with pyridine (0.19 mL, 1.5 equiv) in dichloromethane solvent (5 mL).…”

Dimeric & Polymeric Chiral Schiff Base Complexes and Supported BINOL Complexes as Potential Recyclable Catalysts in Asymmetric Kinetic Resolution and C–C Bond Formation Reactions

“…Among the various methods, enzyme and peptide catalyzed asymmetric cyanohydrin synthesis is well known [128][129][130][131][132][133][134][135][136]. However, from the chemical industry point of view, synthetic chemists are apprehensive about using enzymatic procedures as they require the use of totally different laboratory set-up and reagents from what they are normally trained in.…”