Hydroxyethylcellulose‐<i>graft</i>‐poly (<i>N,N</i>‐dimethylacrylamide) copolymer as a multifunctional separation medium for CE

Peng, Shuhua; Shi, Ronghua; Yang, Runmiao; Zhou, Dan; Wang, Yanmei

doi:10.1002/elps.200800149

Cited by 23 publications

(15 citation statements)

References 14 publications

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“…Increasing the pH to 4.6 resulted in a migration order switch between cytochrome c and lysozyme, which implies that pI can only be used as an indicator of the magnitude of net charge in the region close to pI. This phenomenon was described also in other papers [11,36,37]. At pH 5.2, the neutral and basic proteins were also separated using HEC-g-P4VP as physically adsorbed coating, see Fig.…”

Section: Separation Of Basic and Neutral Proteins With Hec-g-p4vp Coamentioning

confidence: 89%

“…However, the most common approach is based on a coating of the inner capillary wall by covalently linked or physically adsorbed polymers [8] or on addition of small molecules to the BGE, in order to eliminate or tune EOF and/or to prevent analyte adsorption [9,10]. Grafted copolymer hydroxyethylcellulose (HEC)-graft-polydimethylacrylamide, a novel multifunctional separation medium, has recently been evaluated in our group as a suitable coating suppressing EOF and allowing separation of three basic proteins and dsDNA [11]. Polymers that are physically adsorbed to the capillary inner wall to form a coating have several distinct advantages over covalently linked polymer coatings [12].…”

Section: Introductionmentioning

confidence: 99%

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Hydroxyethylcellulose‐graft‐poly (4‐vinylpyridine) as a novel, adsorbed coating for protein separation by CE

2009

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A novel graft copolymer, hydroxyethylcellulose-graft-poly (4-vinylpyridine), used as a physically adsorbed coating of the fused-silica (FS) capillaries, was synthesized by using ceric ammonium nitrate initiator in aqueous nitric acid solution. EOF measurements showed that the above graft copolymer coating of the FS capillaries could suppress EOF effectively compared with the bare FS capillaries. The adsorbed coating exhibited minimal interactions with proteins, providing efficient protein separations with excellent repeatability. Interestingly, the electrical charge of the coated capillary wall could be modulated by varying the pH of the running buffer, which makes possible the analysis of basic and acidic proteins in the same capillary. The effects of pH on suppressing EOF and protein separation were investigated in detail. The results demonstrated that the hydroxyethylcellulose-graft-poly (4-vinylpyridine) copolymer coatings have great potential in the diagnosis and proteomics.

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Section: Separation Of Basic and Neutral Proteins With Hec-g-p4vp Coamentioning

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Hydroxyethylcellulose‐graft‐poly (4‐vinylpyridine) as a novel, adsorbed coating for protein separation by CE

2009

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“…Our previous work 3 has proved that HEC-g-PDMA is an efficient noncovalent coating for basic protein separation. Thus, the separation performance of three different pHs was investigated here by using HEC-g-PDMA-3 coating capillary.…”

Section: Separation Of Basic Proteins With Hec-g-pdma Coatingmentioning

confidence: 99%

“…Hydroxyethylcellulose-graft-poly (N, N-dimethylacrylamide) (HEC-g-PDMA) is an efficient multifunction separation medium for biomolecules by capillary electrophoresis (CE). 3 To optimize the separation performance, the effect of copolymer composition is important to tailor the separation in CE. The conventional synthesis method using initiator in aqueous solution is difficult to characterize the copolymer, such as the grafting density and grafting length.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of hydroxyethylcellulose‐graft‐poly(N, N‐dimethylacrylamide) copolymer by ATRP and as dynamic coating in capillary electrophoresis

Yang¹,

Liu²,

Zheng³

2010

J of Applied Polymer Sci

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Hydroxyethylcellulose-graft-poly (N, Ndimethylacrylamide) was synthesized by successive atom transfer radical polymerization (ATRP) of N,N-dimethylacrylamide (DMA) monomer using HEC-Br as initiator, CuBr and 5,5,7,12,12,4,8, [14]aneN 4 ) as catalyst and ligand, with molar ratio DMA: HEC-Br (CABr): CuBr: Me 6 [14]aneN 4 ¼ 100 : 1 : 1 : 3. HEC-Br macroinitiator was synthesized by esterification of HEC with 2-bromoisobutyryl bromide. GPC and 1 H NMR studies show that the molecular weight of the resulting PDMA increased linearly with the conversion. Within 6 h, the polymerization can reach almost 60% of conversion. The copolymer is applied for the separation of basic proteins in capillary electrophoresis. The results show that this medium has a powerful capability in resisting basic proteins adsorption because the polymer forms noncovalent coating in silica capillaries. With a broad range of pH 2-7, proteins were separated with sufficient efficiencies above 200,000 plates/m.

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“…Neutral polymers are easily rinsed away from the capillary wall, the polymer of PDMA adsorbed to the capillary wall tightly but it would interact with proteins via hydrophobic interactions. To combine advantages of different homopolymers, several kinds of copolymers were synthesized recently, such as poly(1-vinylpyrrolidone-co-2-dimethylaminoethyl methacrylate) (P(VP-co-DMAEMA)) [28], poly (ethylpyrrolidine methacrylate-methylmethacrylate) (EPyM-MMA) [29], poly(N,N-dimethylacrylamide-co-4-(ethyl)-morpholine methacrylamide) (DMA-MAEM) [30], poly (ethylene oxide)-block-poly(4-vinylpyridine) (PEO-b-P4VP) [31], and some derivatized HEC coatings were developed in our laboratory [32][33][34].…”

Section: Introductionmentioning

confidence: 99%

A novel cationic triblock copolymer as noncovalent coating for the separation of proteins by CE

Cao

Zhu

et al. 2011

Electrophoresis

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A novel noncovalent adsorbed coating for CE has been prepared and explored. This coating was based on quaternized poly(2-(dimethylamino)ethyl methacrylate)-block-poly(ethylene oxide)-block-poly(2-(dimethylamino)ethyl methacrylate) (QDED) triblock copolymer which was synthesized by atomic transfer radical polymerization (ATRP) in our laboratory. The polycationic polymer and the negatively charged fused-silica surface attracted each other through electrostatic interactions and hydrogen bonds. It was demonstrated that the coated capillaries provided an electroosmotic flow with reverse direction, and the magnitude of the electroosmotic flow can be modulated by varying the molecular mass of poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA) block and pH value of the buffer. The effects of the molecular mass of PDMAEMA block in QDED triblock copolymer and pH value of the buffer on the separation of basic proteins were investigated in detail. The triblock copolymer coatings showed higher separation efficiency, better migration time repeatability and would apply to wider range of pH than bare fused-silica capillary when used in separating proteins. Proteins from egg white were also separated through this QDED triblock copolymer-coated capillary. These results demonstrated that the QDED triblock copolymer coatings are suitable for analyzing biosamples.

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Hydroxyethylcellulose‐graft‐poly (N,N‐dimethylacrylamide) copolymer as a multifunctional separation medium for CE

Cited by 23 publications

References 14 publications

Hydroxyethylcellulose‐graft‐poly (4‐vinylpyridine) as a novel, adsorbed coating for protein separation by CE

Hydroxyethylcellulose‐graft‐poly (4‐vinylpyridine) as a novel, adsorbed coating for protein separation by CE

Synthesis of hydroxyethylcellulose‐graft‐poly(N, N‐dimethylacrylamide) copolymer by ATRP and as dynamic coating in capillary electrophoresis

A novel cationic triblock copolymer as noncovalent coating for the separation of proteins by CE

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