Hydroxycinnamoyl amides in stem explants from flowering and non‐flowering <i>Nicotiana tabacum</i>

Wyss-Benz, Markus; Streit, Luc; Ebert, E.

doi:10.1111/j.1399-3054.1988.tb00634.x

Cited by 24 publications

(16 citation statements)

References 16 publications

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“…Stem explants out of the inflorescence of 70 d old tobacco plants were used for in vitro culture. Surface-sterilized internodes were cut into pieces of 1 cm length, cross-sectioned, and placed on solidified nutrition medium as described in Wyss-Benz et al (31).…”

Section: Materials and Methods Plantsmentioning

confidence: 99%

“…1). In control explants, FP showed the known pattern (31,32) Figure 7. These tendencies were reproducible upon repeating the experiment.…”

Section: Pal Inhibitorsmentioning

confidence: 98%

“…FP accumulated during callus proliferation while CP accumulated in the second part of culture during flower differentiation (31). However, both stem explants from day-neutral tobacco forming floral buds (31) and stem explants from photoperiodical tobacco forming vegetative shoots (32) exhibited a similar pattern of FP and CP.…”

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confidence: 99%

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Feruloylputrescine and Caffeoylputrescine Are Not Involved in Growth and Floral Bud Formation of Stem Explants from Nicotiana tabacum L. var Xanthi nc

Wyss-Benz¹,

Streit²,

Ebert³

1990

Plant Physiol.

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The role of feruloylputrescine (FP) and of caffeoylputrescine (CP) was investigated in an explant system of stem explants from day-neutral Nicotiana tabacum L. var Xanthi nc. Previously, a correlation between cortical callus formation and increase in FP content, as well as between in vitro flower formation and increase in CP content had been shown. During the explant growth in vitro, the increase of both FP and CP was inhibited by 4-fluor-(1-amino-2-phenylethyl)phosphonic acid and 2-amino-indene-2-phosphonic acid, both inhibitors of phenylalanine ammonia-lyase (EC 4.3.1.5). DL-a-difluoromethylarginine, an inhibitor of arginine decarboxylase (ADC, EC 4.1.1.19), prevented only the increase in FP, while DL-a-difluoromethylornithine, an inhibitor of ornithine decarboxylase (EC 4.1.1.17), reduced only that of CP. Increase in dry weight and the formation of cortical callus and of floral buds of explants were not affected by any of the inhibitors. We conclude, in contrast to earlier hypotheses, that FP and CP do not trigger growth and differentiation in the explants. It seems more likely that FP and CP increase in response to auxin and cytokinin in the media.HCA' are widely distributed in the plant kingdom (12) and are particularly abundant in plant cell and callus cultures (6,15,25). Suggestions pertaining to their role in plants include virus resistance, stress response, and reproduction (15,25). The latter role was forwarded mainly on the grounds that HCA accumulate in floral parts of plants and are absent from cytoplasmic sterile anthers in Zea mays (5, 12-16, 18, 21). HCA were therefore proposed to play a role in induction and differentiation of flowers in plants.The majority of investigators examined the role of HCA as a part of the action of PA. They hydrolyzed plant extracts with HCI and derivatized the conjugated PA with dansylchloride. This method has been used for thin cell layer explants (29) of tobacco to compare in vitro growth responses such as generative or vegetative shoot formation with patterns of free and conjugated PA, the latter including HCA. Torrigiani et

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Section: Materials and Methods Plantsmentioning

confidence: 99%

“…1). In control explants, FP showed the known pattern (31,32) Figure 7. These tendencies were reproducible upon repeating the experiment.…”

Section: Pal Inhibitorsmentioning

confidence: 98%

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Feruloylputrescine and Caffeoylputrescine Are Not Involved in Growth and Floral Bud Formation of Stem Explants from Nicotiana tabacum L. var Xanthi nc

Wyss-Benz¹,

Streit²,

Ebert³

1990

Plant Physiol.

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“…Chemicals, conditions of Nicotiana tabacum L. var. Xanthi nc and in vitro stem culture, extraction of HCAs and HPLC detection were as described in Wyss-Benz et al (1988), unless otherwise indicated.…”

Section: Materials and Methods Generalmentioning

confidence: 99%

Feruloyl‐ and caffeoylputrescine in stem explants from photoperiodically determined Nicotiana species

Wyss-Benz

Streit²,

Ebert³

1989

Physiologia Plantarum

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The distribution and formation of hydroxycinnamoyl amides (HCA) in in vitro grown stem explants from day‐neutral Nicotiana tabacum L. var. Xanthi no were investigated using [14C]‐labelled precursors. Feruloylputrescine (FP) (labelled from [14C]‐putrescine) was continually formed with a decreasing formation rate during a culture period of 35 days. The specific radioactivity, however, remained constant. In contrast, caffeoylputrescine (CP) was labelled in the second part of culture only. [UC]‐Label from putrescine, spermidine, spermine, and cinnamic acid was incorporated into FP and p‐coumaroylspermidine. CP was labelled from [14C]‐putreseine and [14C]‐spermidine only, while diferuloylputrescine (diFP) failed to be labelled by any of the precursors used. Tissue investigations showed that FP is dominant in cortex during formation of cortical callus, while CP showed a sequential gradient from pith to cortical callus to floral buds. Experiments with photoperiodical Nicotiana species were used to investigate the pattern of HCAs in induced and non‐induced plants. The origin of explants had no influence on the pattern of FP and CP but resulted in different growth responses. FP is a marker of cortical callus formation in all explants. CP does not trigger the growth processes observed in the expants since CP also increased in photoperiodically non‐induced explants of tobacco, which stopped growing after the formation of cortical callus.

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“…Some samples were analyzed by the method of Wyss-Benz et al (1988) to determine PAs bound to hydroxycinnamic acids (HCAs).…”

Section: Polyamine Analysismentioning

confidence: 99%

Improved plant regeneration in maize callus cultures after pretreatment with dl-alpha-difluoromethylarginine

Tiburcio

Figueras

Claparols

et al. 1991

Plant Cell Tiss Organ Cult

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The influence of a three-month-long pretreatment with oL-alpha-difluoromethylarginine (DFMA), an irreversible suicide inhibitor of arginine decarboxylase activity (ADC; EC 4.1.1.19), on plant regeneration, protein and polyamine (PA) composition of Zea mays callus cultures has been investigated. A four-fold increase in the number of regenerated plants is obtained after pretreatment with 0.5 mM DFMA. In addition, the regeneration frequency increases 3-fold in the treated calluses and the plants regenerated from such cultures are more developed than the untreated controls. The data obtained on protein and PA contents suggest that a senescence effect is exerted on the calluses grown in the presence of DFMA. However, after DFMA removal a rejuvenation effect occurs on the calluses that may explain the improvement of morphogenic capacity. This study indicates that DFMA pretreatment can be used to increase regeneration efficiency from maize callus cultures.

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Hydroxycinnamoyl amides in stem explants from flowering and non‐flowering Nicotiana tabacum

Cited by 24 publications

References 16 publications

Feruloylputrescine and Caffeoylputrescine Are Not Involved in Growth and Floral Bud Formation of Stem Explants from Nicotiana tabacum L. var Xanthi nc

Feruloylputrescine and Caffeoylputrescine Are Not Involved in Growth and Floral Bud Formation of Stem Explants from Nicotiana tabacum L. var Xanthi nc

Feruloyl‐ and caffeoylputrescine in stem explants from photoperiodically determined Nicotiana species

Improved plant regeneration in maize callus cultures after pretreatment with dl-alpha-difluoromethylarginine

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