Hydroxycinnamic Acids in Wild Blueberry and Effects of Hydroxycinnamic Acids on Apoptosis Induction in Cancer Cell Culture

Abstract

In this study, the fruits and leaves of wild blueberries grown naturally in our country will be evaluated by using different infusion and boiling methods. Blueberry teas; leaves, raw fruit, dried and shredded raw fruit, fruit beans and seedless raw fruit of different infusions were used after boiling them for 1 min, 3 min, 5 min, 7 min, 10 min. Phenolic levels were determined by LC MS/MS technique. The antioxidant and activities of all products in the vitro HCT-116 colon cancer cell line were analyzed by spectrophotometric methods. MDA and TEAC were evaluated for antioxidant activity. Cytotoxicity and viability tests were performed by adding WST-8 (Water Soluble Tetrazolium Salt-8) solution. For apoptosis, TRAIL and Apaf-1 ELISA Kit were used for the activation of caspases of intrinsic and extrinsic pathways.

Variation of Polyphenol Content and Antioxidant Activity in Some Bilberry (Vaccinium myrtillus L.) Populations from Romania

“…In a study regarding the effects of bilberry on cancer cell lines, Durmaz et al [67] found an IC 50 value of 0.0291 mg/mL for caffeic acid, 0.0399 mg/mL for p-coumaric acid, and 0.239 mg/mL for ferulic acid. Choi et al [68] reported that blueberry weakly inhibited diphospho-glucuronosyltransferase, with an IC 50 value of 0.0624 mg/mL.…”