Hydroxychloroquine-Inhibited Dengue Virus Is Associated with Host Defense Machinery

Wang, Li Fong; Lin, You Sheng; Huang, Nan Chieh; Yu, Chia-Yi; Tsai, Wan-Ting; Chen, Jih Jung; Kubota, Takeshi; Matsuoka, Masao; Chen, Siang Ru; Yang, Chih Shiang; Lu, Ruo Wei; Lin, Yi-Ling; Chang, Tsun-Hsu

doi:10.1089/jir.2014.0038

Cited by 93 publications

(97 citation statements)

References 67 publications

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“…Cells cultured in 12-well plates were transfected with NF-κB-Luc reporter plasmids (46) by TurboFect. pRL-TK (Promega), encoding Renilla luciferase under a herpes simplex virus thymidine kinase promoter, was an internal control.…”

Section: Methodsmentioning

confidence: 99%

Lipopolysaccharide Attenuates Induction of Proallergic Cytokines, Thymic Stromal Lymphopoietin, and Interleukin 33 in Respiratory Epithelial Cells Stimulated with PolyI:C and Human Parechovirus

Lin

Cheng

et al. 2016

Front. Immunol.

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Epidemiological studies based on the “hygiene hypothesis” declare that the level of childhood exposure to environmental microbial products is inversely related to the incidence of allergic diseases in later life. Multiple types of immune cell-mediated immune regulation networks support the hygiene hypothesis. Epithelial cells are the first line of response to microbial products in the environment and bridge the innate and adaptive immune systems; however, their role in the hygiene hypothesis is unknown. To demonstrate the hygiene hypothesis in airway epithelial cells, we examined the effect of lipopolysaccharide (LPS; toll-like receptor 4 ligand) on the expression of the proallergic cytokines thymic stromal lymphopoietin (TSLP) and interleukin 33 (IL33) in H292 cells (pulmonary mucoepidermoid carcinoma cells). Stimulation with the TLR ligand polyI:C and human parechovirus type 1 (HPeV1) but not LPS-induced TSLP and IL33 through interferon regulatory factor 3 (IRF3) and NF-κB activity, which was further validated by using inhibitors (dexamethasone and Bay 11-7082) and short hairpin RNA-mediated gene knockdown. Importantly, polyI:C and HPeV1-stimulated TSLP and IL33 induction was reduced by LPS treatment by attenuating TANK-binding kinase 1, IRF3, and NF-κB activation. Interestingly, the basal mRNA levels of TLR signaling proteins were downregulated with long-term LPS treatment of H292 cells, which suggests that such long-term exposure modulates the expression of innate immunity signaling molecules in airway epithelial cells to mitigate the allergic response. In contrast to the effects of LPS treatment, the alarmin high-mobility group protein B1 acts in synergy with polyI:C to promote TSLP and IL33 expression. Our data support part of the hygiene hypothesis in airway epithelia cells in vitro. In addition to therapeutic targeting of TSLP and IL33, local application of non-pathogenic LPS may be a rational strategy to prevent allergies.

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Section: Methodsmentioning

confidence: 99%

Lipopolysaccharide Attenuates Induction of Proallergic Cytokines, Thymic Stromal Lymphopoietin, and Interleukin 33 in Respiratory Epithelial Cells Stimulated with PolyI:C and Human Parechovirus

Lin

Cheng

et al. 2016

Front. Immunol.

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“…Previous studies reported that CQ/HCQ possess a broad spectrum of antiv iral effects on a variety of viruses as diverse as human immunodeficiency virus (HIV) [30], Marburg virus, Zika virus [31], dengue virus [32], Ebola virus [33], and SARS-Co V-1 [34,35], etc. CQ and HCQ can interfere with the binding of viral particles to their cellular cell surface receptor or the pH-dependent endosome-med iated viral entry of enveloped viruses to inhibit the v iral cycle [32]. They can also interfere with the post-translational modification of viral proteins or impair the proper maturation of viral p rotein by pH modulation [36].…”

Section: Jak Inhibitorsmentioning

confidence: 99%

The use of anti-inflammatory drugs in the treatment of people with severe coronavirus disease 2019 (COVID-19): The Perspectives of clinical immunologists from China

Zhang¹,

Zhao²,

Zhang³

et al. 2020

Clinical Immunology

1,171

1,341

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The pandemic outbreak of coronavirus disease 2019 is rap idly spreading all over the world. Reports from China showed that about 20% of patients developed severe disease, resulting in a fatality of 4%. In the past two months, we clinical immunologists participated in mu lti-rounds of MDT (mult idiscipline team) discussion on the anti-inflammat ion management of critical ill COVID-19 patients, with our colleagues dispatched from Ch inese leading PUM C Hospital to Wuhan to admit and treat the most severe patients . Here, fro m the perspective of clinical immunologists, we will discuss the clin ical and immunological characteristics of severe patients, and summarize the current evidence and share our experience in anti-inflammat ion treatment, including glucocorticoids, IL-6 antagonist, JAK inhibitors and choloroquine/hydrocholoroquine, of patients with severe COVID-19 that may have an impaired immune system.

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“…Several antimalarial drugs that contain a quinoline scaffold were evaluated for their antiviral activity against DENV [260][261][262] or WNV 260 . Amodiaquine inhibited DENV-2, DENV-4 and WNV replication but with relatively poor selectivity 260 .…”

Section: Polyamine Synthesis Inhibitorsmentioning

confidence: 99%

Broad-spectrum agents for flaviviral infections: dengue, Zika and beyond

Boldescu

Behnam

Vasilakis³

et al. 2017

Nat Rev Drug Discov

242

217

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Infections with flaviviruses, such as dengue, West Nile virus, and the recently re-emerging Zika virus are an increasing and probably lasting global risk. This review summarizes and comments on the opportunities for broad-spectrum agents that are active against a range of flaviviruses. Broad-spectrum activity would be particularly desirable as preparatory measure for the next flaviviral epidemic that could emerge from as-yet-unknown or neglected viruses. Potential target sites for broad-spectrum anti-flaviviral compounds include viral proteins and host mechanisms that are exploited by these viruses during entry and replication. A variety of compounds with broad-spectrum antiviral activity have already been identified by target-specific or phenotypic assays. For some other compound classes, broad-spectrum activity can be anticipated because of their mode of action and molecular target(s).

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Hydroxychloroquine-Inhibited Dengue Virus Is Associated with Host Defense Machinery

Cited by 93 publications

References 67 publications

Lipopolysaccharide Attenuates Induction of Proallergic Cytokines, Thymic Stromal Lymphopoietin, and Interleukin 33 in Respiratory Epithelial Cells Stimulated with PolyI:C and Human Parechovirus

Lipopolysaccharide Attenuates Induction of Proallergic Cytokines, Thymic Stromal Lymphopoietin, and Interleukin 33 in Respiratory Epithelial Cells Stimulated with PolyI:C and Human Parechovirus

The use of anti-inflammatory drugs in the treatment of people with severe coronavirus disease 2019 (COVID-19): The Perspectives of clinical immunologists from China

Broad-spectrum agents for flaviviral infections: dengue, Zika and beyond

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