Hydroxyapatite/sericin composites: A simple synthesis route under near-physiological conditions of temperature and pH and preliminary study of the effect of sericin on the biomineralization process

Veiga, Anabela; Castro, Filipa; Cunha-Reis, Cassilda; Sousa, Aureliana; Oliveira, Ana L.; Rocha, Fernando

doi:10.1016/j.msec.2019.110400

Cited by 29 publications

(51 citation statements)

References 68 publications

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“…In addition, HAp is very well-known biocompatible substitution for the essential mineral constituents of skeletal bones that can be utilized as orthopedic surgeries and tooth implant [20,21]. HAp is considered to be extremely bioactive, osteoconductive, biocompatible, non-toxic, non-immunogenic, and non-inflammatory [22,23].…”

Section: Abstract a R T I C L E I N F O R M A T I O Nmentioning

confidence: 99%

Hydroxyapatite Consolidated by Zirconia: Applications for Dental Implant

Sharifianjazi

Pakseresht

Asl

et al. 2020

jcc

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Section: Abstract a R T I C L E I N F O R M A T I O Nmentioning

confidence: 99%

Hydroxyapatite Consolidated by Zirconia: Applications for Dental Implant

Sharifianjazi

Pakseresht

Asl

et al. 2020

jcc

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“…HAp/SS precipitation process To produce HAp/SS composites, a simple precipitation route that only requires three reagents was used (SS, calcium and phosphate precursors). In the methodology, validated in a previous work, 18 HAp/SS suspensions were obtained under near-physiological conditions of pH and temperature (Table 1). HAp is the least soluble CaP phase at 37°C and for a pH range from 4 to 12, which is the most stable CaP phase for the studied operating conditions.…”

Section: Resultsmentioning

confidence: 99%

“…HAp/SS synthesis was carried out according to the procedure described by Veiga et al 18 . Briefly, a solution containing CaCl 2 ⋅2H 2 O (Merck, 99.5%) (0.02 mol L –1 ) and SS obtained by extraction in boiling water was added to a solution of Na 2 HPO 4 (Sigma‐Aldrich, 99.0%) (0.012 mol L –1 ).…”

Section: Methodsmentioning

confidence: 99%

“…Precipitation of HAp/SS composites HAp/SS synthesis was carried out according to the procedure described by Veiga et al 18 Briefly, a solution containing CaCl 2 Á2H 2 O (Merck, 99.5%) (0.02 mol L -1 ) and SS obtained by extraction in boiling water was added to a solution of Na 2 HPO 4 (Sigma-Aldrich, 99.0%) (0.012 mol L -1 ). Various solutions were prepared by varying the SS concentration (HAp, HAp/SS1 and HAp/SS2, with 0, 0.1 and 0.01 g L −1 f SS, respectively).…”

Section: Meso-oscillatory Flow Reactormentioning

confidence: 99%

“…[15][16][17] Despite their properties, studies focusing on HAp/SS biomaterials are still scarce. 18 Reported HAp/SS nanocomposites are commonly obtained through wet chemical precipitation. 15,[19][20][21][22][23] This methodology is conventionally carried out using a stirred tank batch reactor (ST) due to its simplicity, versatility as well as the minimal operation costs involved.…”

Section: Introductionmentioning

confidence: 99%

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High efficient strategy for the production of hydroxyapatite/silk sericin nanocomposites

Veiga

Castro

Oliveira

et al. 2020

J of Chemical Tech & Biotech

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BACKGROUND: Sericin (SS) induces nucleation of bone-like hydroxyapatite (HAp) when used as an organic matrix. HAp/SS nanocomposites have been conventionally synthesized through precipitation in stirred tank reactors (STs). Despite its simplicity, this process is time consuming and presents difficulties in scale-up. In our study, HAp/SS nanocomposites were successfully synthesized in a ST and in a meso-oscillatory flow reactor (meso-OFR), to compare the efficiency of both reactors and to study HAp mineralization using SS as a template. RESULTS: The production of stable HAp, indicated by pH stabilization, was achieved after 180 min in the ST and after 30 min in the meso-OFR. X-ray diffraction and Fourier transform infrared analyses showed that the particles obtained in both reactors are HAp/SS nanocomposites with low crystallinity. Scanning electron microscopy evidenced the formation of rod-and plateshaped nanoparticles and revealed that the presence of SS led to the production of larger particles. The latter observation was confirmed by laser diffraction. Additionally, increasing SS concentration resulted in the formation of more plate-like particles. CONCLUSIONS: Precipitation is more efficient in the meso-OFR, HAp/SS being obtained four times faster. The presence and concentration of SS led to differences in the size and morphology of the synthesized particles, suggesting a critical role of SS in the mineralization process. This work reports a new approach for the manufacture of high-added-value nanocomposites with similar characteristics to biological bone and the results of a study of the influence of SS as an organic component in HAp nucleation. Further, the use of this protein and technology leads to significant waste minimization.

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Novel 8%‐TiO₂‐nanoparticle‐reinforced dense polycrystalline bovine hydroxyapatite bioceramic

Azevedo-Silva

Ferrairo

Pires

et al. 2022

Int J Ceramic Engine & Sci

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This study aimed to produce and characterize the microstructure and mechanical properties of dense polycrystalline bovine hydroxyapatite (DPBHA) bioceramics with 5% and 8% of TiO 2 nanoparticles after final synthetization for future use in dental implants. Structural characterization was obtained from analyzes by Fourier transform infrared spectroscopy, X-ray diffraction, scanning electron microscope, energy dispersive spectroscopy, and relative density and apparent porosity. The mechanical characterization was performed by measuring the fracture toughness after three-point flexural strength (FS) test. The microstructural characterization results showed no secondary phase formation and nonhomogeneous nanoparticle dispersion in HA matrix. DPBHA/Np8% (2.9 ± 0.09 g/cm 3 ) exhibited significantly greater density than DPBHA (2.7 ± 0.03 g/cm 3 ) (p = 0.011) and DPBHA/Np5% (2.7 ± 0.05 g/cm 3 ) (p = 0.041). DPBHA (0.9%) had the smallest porosity followed by DPBHA/Np8% (3.4%). DPBHA/Np5% (4.5%) exhibited the greatest proportion of pores. Pure HA (51.7 ± 10.3 MPa) and DPBHA/Np8% (47.4 ± 6.4 MPa) had significant greater FS (p < 0.001) than DPBHA/Np5% (28.8 ± 3.1 MPa). DPBHA (0.43 ± 0.01 MPa m 1/2 ) and DPBHA/Np8% (0.40 ± 0.06 MPa m 1/2 ) presented greater KIc than DPBHA/Np5% (0.23 ± 0.02 MPa m 1/2 ) (p < 0.003; p < 0.007). In conclusion, 8% TiO 2 nanoparticle addition to this synthesis would be a promising HA blend, as mechanical properties were similar, and the relative density/apparent porosity showed superior results than those of the DPBHA.

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Hydroxyapatite/sericin composites: A simple synthesis route under near-physiological conditions of temperature and pH and preliminary study of the effect of sericin on the biomineralization process

Cited by 29 publications

References 68 publications

Hydroxyapatite Consolidated by Zirconia: Applications for Dental Implant

Hydroxyapatite Consolidated by Zirconia: Applications for Dental Implant

High efficient strategy for the production of hydroxyapatite/silk sericin nanocomposites

Novel 8%‐TiO₂‐nanoparticle‐reinforced dense polycrystalline bovine hydroxyapatite bioceramic

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Hydroxyapatite/sericin composites: A simple synthesis route under near-physiological conditions of temperature and pH and preliminary study of the effect of sericin on the biomineralization process

Cited by 29 publications

References 68 publications

Hydroxyapatite Consolidated by Zirconia: Applications for Dental Implant

Hydroxyapatite Consolidated by Zirconia: Applications for Dental Implant

High efficient strategy for the production of hydroxyapatite/silk sericin nanocomposites

Novel 8%‐TiO2‐nanoparticle‐reinforced dense polycrystalline bovine hydroxyapatite bioceramic

Contact Info

Product

Resources

About

Novel 8%‐TiO₂‐nanoparticle‐reinforced dense polycrystalline bovine hydroxyapatite bioceramic