Hydroxyapatite nanophases augmented with selenium and manganese ions for bone regeneration: Physiochemical, microstructural and biological characterization

Muthusamy, Shalini; Mahendiran, Balaji; Sampath, Sowndarya; Jaisankar, Sellamuthu N.; Anandasadagopan, Suresh Kumar; Krishnakumar, Gopal Shankar

doi:10.1016/j.msec.2021.112149

Cited by 17 publications

(12 citation statements)

References 57 publications

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“…[ 23,25 ] Mn can improve the mechanical properties of hydroxyapatite, promote osteoblast proliferation and mineralization, and inhibit osteoclastic effects. [ 26 ]…”

Section: Resultsmentioning

confidence: 99%

Manganese Enhances the Osteogenic Effect of Silicon‐Hydroxyapatite Nanowires by Targeting T Lymphocyte Polarization

Li,

Zhu,

Zhang

et al. 2023

Advanced Science

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Biomaterials encounter considerable challenges in extensive bone defect regeneration. The amelioration of outcomes may be attainable through the orchestrated modulation of both innate and adaptive immunity. Silicon‐hydroxyapatite, for instance, which solely focuses on regulating innate immunity, is inadequate for long‐term bone regeneration. Herein, extra manganese (Mn)‐doping is utilized for enhancing the osteogenic ability by mediating adaptive immunity. Intriguingly, Mn‐doping engenders heightened recruitment of CD4+ T cells to the bone defect site, concurrently manifesting escalated T helper (Th) 2 polarization and an abatement in Th1 cell polarization. This consequential immune milieu yields a collaborative elevation of interleukin 4, secreted by Th2 cells, coupled with attenuated interferon gamma, secreted by Th1 cells. This orchestrated interplay distinctly fosters the osteogenesis of bone marrow stromal cells and effectuates consequential regeneration of the mandibular bone defect. The modulatory mechanism of Th1/Th2 balance lies primarily in the indispensable role of manganese superoxide dismutase (MnSOD) and the phosphorylation of adenosine 5′‐monophosphate‐activated protein kinase (AMPK). In conclusion, this study highlights the transformative potential of Mn‐doping in amplifying the osteogenic efficacy of silicon‐hydroxyapatite nanowires by regulating T cell‐mediated adaptive immunity via the MnSOD/AMPK pathway, thereby creating an anti‐inflammatory milieu favorable for bone regeneration.

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“…[ 23,25 ] Mn can improve the mechanical properties of hydroxyapatite, promote osteoblast proliferation and mineralization, and inhibit osteoclastic effects. [ 26 ]…”

Section: Resultsmentioning

confidence: 99%

Manganese Enhances the Osteogenic Effect of Silicon‐Hydroxyapatite Nanowires by Targeting T Lymphocyte Polarization

Li,

Zhu,

Zhang

et al. 2023

Advanced Science

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“…Particle fusion also leads to the formation of loose aggregates. 49 , 50 However, because of the dense formed clusters of both pure and doped HA particles, determining the size of a single particle was challenging. Spherical powders offer higher rheological qualities than irregular powders and less cytotoxicity than needle and plate shaped nanohydroxyapatite, making them ideal for medical applications.…”

Section: Resultsmentioning

confidence: 99%

Selenium- and/or copper-substituted hydroxyapatite: A bioceramic substrate for biomedical applications

Korowash,

Keskin-Erdogan,

Hemdan

et al. 2023

J Biomater Appl

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Atomic substitution or doping of a bioceramic material hydroxyapatite (HA) with specific ions is an appealing approach for improving its biocompatibility and activity, as well as imparting antibacterial properties. In this study, selenium- and/or copper-substituted hydroxyapatite powders were synthesized by an aqueous precipitation method and using the freeze-drying technique. The molar concentrations of constituents were calculated based on the proposed mechanism whereby selenium (Se4+) ions partially substitute phosphorus (P5+) sites, and copper (Cu2+) ions partially substitute (Ca2+) sites in the HA lattice. Dried precipitated samples were characterized using Inductively coupled plasma optical emission spectroscopy (ICP-OES), X-ray diffraction analysis (XRD), Fourier-transform infrared spectroscopy (FTIR) and Field-emission scanning electron microscopy with energy dispersive X-ray spectroscopy (FESEM-EDX). Accordingly, substitution of Se4+ and/or Cu2+ ions took place in the crystal lattice of HA without the formation of any impurities. The presence of sulphur (S2-) ions in the hydroxyapatite was detected by ICP-OES in all samples with copper substituted in the lattice. The cytotoxicity of the powders on osteoblastic (MC3T3-E1) cells was evaluated in vitro. Selenium substituted hydroxyapatite (SeHA), at the concentration (200 μg/mL), demonstrated higher populations of the live cells than that of control (cells without powders), suggesting that selenium may stimulate the proliferation of these cells. In addition, the copper substituted hydroxyapatite (CuHA) and the selenium and copper substituted hydroxyapatite (SeCuHA) at the concentrations (200 and 300 μg/mL) and (200 μg/mL), respectively demonstrated better results than the unsubstituted HA. Antimicrobial activity was assessed using a well-diffusion method against Streptococcus mutans and Candida albicans, and superior results has obtained with SeCuHA samples. Presented findings imply that selenium and/or copper substituted modified hydroxyapatite nanoparticles, may be an attractive antimicrobial and cytocompatible substrate to be considered for use in a range of translational applications.

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“…According to the previous publications, trace element doped HA including Sr-HA, 20,21 Si-HA, 34 Zn-HA, 35 Mg-HA, 36 and Se-HA 37 could accelerate cell proliferation and differentiation in vitro , and bone defect regeneration in vivo . For instance, Sr was indispensable in bone metabolism by stimulating bone formation and restraining bone resorption.…”

Section: Discussionmentioning

confidence: 96%

A facile way to construct Sr-doped apatite coating on the surface of 3D printed scaffolds to improve osteogenic effect

Chen

Wang

2022

J Biomater Appl

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Bone-like apatite coating fabricated by biomineralization process is a facile way for surface modification of porous scaffolds to improve interfacial behaviors and thus facilitate cell attachment, proliferation, and differentiation for bone tissue engineering. In this study, a Sr-containing calcium phosphate solution was made and used to construct a thick layer of Sr-doped bone-like apatite on the surface of 3D printed scaffolds via biomineralization process. Importantly, Sr-doped bone-like apatite could form and fully cover the 3D printed scaffolds surface in hours. The characterization results indicated that Sr2+ ions successfully replaced Ca2+ ions in bone-like apatite and the molar ratio of Sr/(Ca+Sr) was up to 8.2%. Furthermore, the Sr-doped apatite coating increased the compressive strength and Young’s modulus of composite scaffolds. The compatibility and bioactivity of mineralized scaffolds were evaluated by cell attachment, proliferation, and differentiation of MC3T3-E1 cells. It was found that Sr-doped apatite coating could gradually release Sr2+ ions and further promote cell attachment, proliferation rate, and the expression of alkaline phosphatase activity and osteogenic related genes, such as collagen type I (Col I), Runt-related transcription factor 2 (Runx-2), osteopontin, and osterix. Therefore, the Sr-doped apatite coating fabricated by this facile and rapid biomineralization process offers a new strategy to modify 3D printed porous scaffolds with significantly improved mechanical and biological properties for bone tissue engineering applications.

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Hydroxyapatite nanophases augmented with selenium and manganese ions for bone regeneration: Physiochemical, microstructural and biological characterization

Cited by 17 publications

References 57 publications

Manganese Enhances the Osteogenic Effect of Silicon‐Hydroxyapatite Nanowires by Targeting T Lymphocyte Polarization

Manganese Enhances the Osteogenic Effect of Silicon‐Hydroxyapatite Nanowires by Targeting T Lymphocyte Polarization

Selenium- and/or copper-substituted hydroxyapatite: A bioceramic substrate for biomedical applications

A facile way to construct Sr-doped apatite coating on the surface of 3D printed scaffolds to improve osteogenic effect

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