Hydroxyapatite nanoparticles modified with metal ions for xylanase immobilization

Coutinho, Thamara Carvalho; Tardioli, Paulo Waldir; Farinas, Cristiane S.

doi:10.1016/j.ijbiomac.2020.02.058

Cited by 27 publications

(27 citation statements)

References 34 publications

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“…In terms of the enzyme loadings, the greatest amounts of protein were adsorbed on the 2:1 HA/CoFe 2 O 4 support, with values of 14.7, 6.9, and 4.5 mg protein/g support for β-glucosidase, xylanase, and phytase, respectively. The same order of adsorption was reported previously for the immobilization of these enzymes on pure HA, although the adsorptions were higher, with values of 32, 15, and 6 mg protein/g support for β-glucosidase, xylanase, and phytase, respectively. − The lower adsorption capacities of the HA/CoFe 2 O 4 composites, compared to pure HA, were probably due to the decreased surface areas of the composites after incorporation of cobalt ferrite in the HA (Table ).…”

Section: Resultssupporting

confidence: 73%

“…Characterization of the Magnetic Hydroxyapatite Supports (HA/CoFe 2 O 4 ). The FEG-SEM (field emission gun− scanning electron microscopy) technique was used to analyze the morphologies of the nanoparticle composites, 23 while the energydispersive X-ray spectroscopy (EDS) technique was used to qualitatively analyze the chemical compositions of the nanoparticle composites. 21 The crystallinity characteristics of the hydroxyapatite, the cobalt ferrite, and the HA/CoFe 2 O 4 composites were investigated by Xray diffraction (XRD) measurements.…”

Section: Methodsmentioning

confidence: 99%

“…41,42 Recent studies have shown the potential of exploring the fast and stable interactions resulting from the chelation reaction between the calcium ions of HA and the carboxylic acid groups of proteins for enzyme immobilization. 19,23 At the same time, other electron pair donors on the βglucosidase, xylanase, and phytase surfaces, such as His, Arg, Trp, and Lys, could have participated in the formation of metal chelates involving Ca 2+ , Fe 3+ , and Co 2+ on the support matrix. Figure 6 shows the three-dimensional structures of these three enzymes, highlighting the amino acid residues capable of chelating with the metals present in the structure of the synthesized magnetic nanocomposites of HA/CoFe 2 O 4 .…”

Section: Desorption Experimentsmentioning

confidence: 99%

“…For phytase, the derivative showed a broader profile of activity according to pH and temperature, with higher stability than the free enzyme at high temperatures, demonstrating its potential application in animal feed . For xylanase, the enzyme showed a higher affinity for the HA support modified with copper, demonstrating promising industrial applications . Despite these excellent results, drawbacks remained concerning the recovery of these biocatalysts, which could be resolved using magnetic supports.…”

Section: Introductionmentioning

confidence: 99%

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Hydroxyapatite-CoFe₂O₄ Magnetic Nanoparticle Composites for Industrial Enzyme Immobilization, Use, and Recovery

Coutinho

Malafatti

Paris

et al. 2020

ACS Appl. Nano Mater.

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Hydroxyapatite (HA) is an inorganic material with high ability to interact with proteins and has been recently explored as a support for enzyme immobilization. However, there are still some drawbacks concerning the recovery of these biocatalysts, which could be overcome using magnetic supports. Cobalt ferrite (CoFe 2 O 4 ) is a purely magnetic material, which offers excellent chemical stability, ease of synthesis, and mechanical hardness, being a promising candidate to form composites with HA and produce magnetic HA nanoparticles. Therefore, investigation of synthesis procedures and applications of HA/CoFe 2 O 4 composites to facilitate enzyme recovery by means of a magnetic field could find interest in a broad spectrum of biotechnological processes. Here, the co-precipitation method was used to synthesize HA/CoFe 2 O 4 composites with different HA/CoFe 2 O 4 mass ratios, for application as supports for enzyme immobilization. The enzymes β-glucosidase, phytase, and xylanase were selected for proof of concept due to their wide range of industrial applications. The composite with the highest cobalt ferrite content (2:1 mass ratio) was highly effective for immobilization of the three different enzymes, with immobilization yields (IYs) of 70−100% and recovered activities of 78−100%. The biocatalysts could be easily recovered from the reaction media by both centrifugation and application of an external magnetic field, demonstrating their potential for use in industrial processes. The materials exhibited good reusability, especially in the case of the βglucosidase biocatalyst, which could be reused 10 times, maintaining around 70% of its initial activity.

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Section: Resultssupporting

confidence: 73%

Section: Methodsmentioning

confidence: 99%

Section: Desorption Experimentsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Hydroxyapatite-CoFe₂O₄ Magnetic Nanoparticle Composites for Industrial Enzyme Immobilization, Use, and Recovery

Coutinho

Malafatti

Paris

et al. 2020

ACS Appl. Nano Mater.

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“…As can be seen from the FDA site, there is a significant need for the development of new theranostic tools that target the CNS tumor scope, necessitating continued efforts to develop safe and effective theranostic agents and delivery methods. Some literature has provided evidence that there are possible solutions for improving delivery and decreasing toxicity of metal-based imaging agents, e.g., use of hydroxyapatite (HA) nanoparticles [ 189 , 190 , 191 ]. The HA is nontoxic and supports the effective pharmaceutical development of therapeutic agents.…”

Section: Future Directionsmentioning

confidence: 99%

Nanoparticles as a Tool in Neuro-Oncology Theranostics

et al. 2021

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The rapid growth of nanotechnology and the development of novel nanomaterials with unique physicochemical characteristics provides potential for the utility of nanomaterials in theranostics, including neuroimaging, for identifying neurodegenerative changes or central nervous system malignancy. Here we present a systematic and thorough review of the current evidence pertaining to the imaging characteristics of various nanomaterials, their associated toxicity profiles, and mechanisms for enhancing tropism in an effort to demonstrate the utility of nanoparticles as an imaging tool in neuro-oncology. Particular attention is given to carbon-based and metal oxide nanoparticles and their theranostic utility in MRI, CT, photoacoustic imaging, PET imaging, fluorescent and NIR fluorescent imaging, and SPECT imaging.

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Metal ion‐mediated immobilization of catalase on poly(ε‐caprolactone)/polyethyleneimine electrospun nanofibers

Çetin

2023

J of Applied Polymer Sci

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A common industrial byproduct known as hydrogen peroxide, which is an essential intermediate, is associated with oxidative damage to brain cells and a number of neurodegenerative disorders. Although catalases are enzymes capable of decomposing hydrogen peroxide into water and oxygen, the low stability of free enzymes limits their industrial use. In the current study, poly(ε‐caprolactone)/polyethyleneimine nanofibers were fabricated using the electrospinning process and catalase was subsequently immobilized via coordination of Cu2+ ions. The characterization of the catalase‐immobilized nanofibers and their catalytic activity for hydrogen peroxide decomposition was evaluated by comparing the free catalase. The studies conducted in the pH range of 5.0–8.5 revealed that the catalase‐immobilized nanofibers had their highest levels of enzyme activity at pH 7.0. When compared to free catalase, it was shown that the thermal stability and storage stability of catalase‐immobilized nanofibers were significantly improved. Catalase‐immobilized nanofibers showed significant reusability, where activity was maintained by around 60% after five repeating cycles. The findings showed that catalase‐immobilized PCL/PEI‐Cu2+ nanofibers had a high potential for various industrial applications as a substitute for the existing techniques.

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Hydroxyapatite nanoparticles modified with metal ions for xylanase immobilization

Cited by 27 publications

References 34 publications

Hydroxyapatite-CoFe₂O₄ Magnetic Nanoparticle Composites for Industrial Enzyme Immobilization, Use, and Recovery

Hydroxyapatite-CoFe₂O₄ Magnetic Nanoparticle Composites for Industrial Enzyme Immobilization, Use, and Recovery

Nanoparticles as a Tool in Neuro-Oncology Theranostics

Metal ion‐mediated immobilization of catalase on poly(ε‐caprolactone)/polyethyleneimine electrospun nanofibers

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Hydroxyapatite nanoparticles modified with metal ions for xylanase immobilization

Cited by 27 publications

References 34 publications

Hydroxyapatite-CoFe2O4 Magnetic Nanoparticle Composites for Industrial Enzyme Immobilization, Use, and Recovery

Hydroxyapatite-CoFe2O4 Magnetic Nanoparticle Composites for Industrial Enzyme Immobilization, Use, and Recovery

Nanoparticles as a Tool in Neuro-Oncology Theranostics

Metal ion‐mediated immobilization of catalase on poly(ε‐caprolactone)/polyethyleneimine electrospun nanofibers

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Product

Resources

About

Hydroxyapatite-CoFe₂O₄ Magnetic Nanoparticle Composites for Industrial Enzyme Immobilization, Use, and Recovery

Hydroxyapatite-CoFe₂O₄ Magnetic Nanoparticle Composites for Industrial Enzyme Immobilization, Use, and Recovery