Hydrothermal synthesis of hydroxyapatite nanoparticles with various counterions as templates

Abstract

Hydrothermal synthesis proves to be a feasible route to fabricate hydroxyapatite nanoparticles. The study reports the combinational effects of temperature and various counterions, such as SO 4 2¹, Cl ¹ , and K + , on the morphology of hydroxyapatite nanoparticles. The resultant nanoparticles are investigated using powder X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and transmission electron microscopy (TEM). The results show that the effects of temperature and different counterions are significant, and hydroxyapatite nanoparticles with the morphology of nano-platelet and nano-rod are produced without the addition of any organic surfactants. The process described will be beneficial to industry by lowering production costs, and contributing to ever increasing need to eliminate contamination to environment.

Synthesis and Optimization of Colloidal Hydroxyapatite Nanoparticles by Hydrothermal Processes

“…26 In that context, it was very important to establish a clear relationship between experimental conditions and particle morphology and crystallinity. The crystalline structures of the samples synthesized with different experimental conditions are shown in Figure 1.…”

“…This study demonstrated that the HA nanoparticles with modified shape could be prepared by using the combined effects of temperature and counter ions, which are simple, inexpensive and eco-friendly. 23,26 Conclusions HA nanoparticles were successfully synthesized by hydrothermal method using friendly synthesis conditions. Due the conditions and experiments studied, homogeneous colloidal nanoparticles were obtained in their form and crystallinity, having a selective chemical composition, being this a reproducible and simpler method.…”

“…Another advantage of hydrothermal synthesis is that it does not require the final calcination step to obtain a pure crystalline material. 26,27 The applications of HA nanoparticles are influenced by the shape, crystallinity and purity of the synthesized material. Therefore, it is important to control the shape, size and crystalline structure of the nanoparticles according to the different synthesis conditions.…”

Augmented drug resistance of osteosarcoma cells within decalcified bone matrix scaffold: The role of glutamine metabolism

“…Calcium content in dBMS was measured using the ethylenediaminetetraacetic acid (EDTA) complexometric titration method 14 . For immunohistochemistry(IHC) of collagen type I (COLI), sections were performed with 0.1% trypsin without EDTA for 2 h at 37°C for antigen retrieval.…”

“…Calcium content in dBMS was measured using the ethylenediaminetetraacetic acid (EDTA) complexometric titration method. 14 For immunohistochemistry(IHC) of collagen type I (COLI), sections were performed with 0.1% trypsin without EDTA for 2 h at 37 C for antigen retrieval. After blocking with 5% BSA for 1 h at room temperature, sections were incubated with primary rabbit anti-human COLI antibody (Servicebio, GB11022-1, 1:200, China) at 4 C overnight.…”