Hydrothermal processing of 3D-printed calcium phosphate scaffolds enhances bone formation in vivo: a comparison with biomimetic treatment

Raymond, Yago; Bonany, Mar; Lehmann, Cyril; Thorel, Emilie; Benítez, Raúl; Franch, Jordi; Español, Montserrat; Solé‐Martí, Xavi; Manzanares-Céspedes, María Cristina; Canal, Cristina; Ginebra, Maria‐Pau

doi:10.1016/j.actbio.2021.09.001

Cited by 15 publications

(14 citation statements)

References 76 publications

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“…The in vitro behavior showed good agreement with the in vivo results, where both the plasma‐treated composite biomaterial and its combination with direct plasma treatment displayed good bone formation, as compared to the control robocasted scaffolds that have previously shown good in vivo performance. [ 40,61 ] Here, the BVF, that is the newly formed bone in vivo, was equivalent in all samples (Figure 8a). Furthermore, histological and SEM analysis (Figures 6 and 7) showed similar results in all cases: no signs of fibrous tissue were detected and newly formed bone was in direct contact with the material for all samples, suggesting that the RONS released from the composite biomaterial did not affect nonmalignant cells (in accordance with the in vitro assay), allowing bone ingrowth.…”

Section: Discussionmentioning

confidence: 86%

“…An in‐depth characterization of the pristine scaffolds can be found in a previous work. [ 40 ] Therefore, this section will focus on the characterization of the novel composite biomaterial composed of the CDHA scaffold infiltrated with plasma‐treated Gel/Alg.…”

Section: Resultsmentioning

confidence: 99%

“…A median filtering process with a disk-shaped structuring element of radius 50 µm was applied to the segmented volume to remove residual noise and quantitative information was extracted from each region (Figure 2a). More information about the segmentation procedure can be found in Raymond et al [40] and the source code in Python is available from a GitHub repository. [41] To evaluate the accuracy of the automated segmentation, 20 μCT images were selected randomly and manually segmented using ImageJ (Open Source software).…”

Section: Segmentation Algorithmmentioning

confidence: 99%

See 2 more Smart Citations

Ceramic‐hydrogel composite as carrier for cold‐plasma reactive‐species: Safety and osteogenic capacity in vivo

Solé‐Martí

Labay

Raymond

et al. 2022

Plasma Processes & Polymers

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Plasma‐treated hydrogels have been put forward as a potential selective osteosarcoma therapy through the release of reactive species to the diseased site. To allow their translation to the clinics, it is crucial to show that the oxidative stress delivered by such hydrogels does not adversely affect healthy tissues. This is evaluated here by investigating the in vivo performance of a robocasted calcium phosphate cement infiltrated by a plasma‐treated hydrogel. The plasma‐treated composite implanted in a critical size bone defect of healthy rabbits revealed its safety, allowing equivalent bone ingrowth compared to the control scaffolds and to that of direct plasma treatment of the bone defect. This opens the door for using composite biomaterials containing plasma‐generated reactive species in bone therapies.

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Section: Discussionmentioning

confidence: 86%

Section: Resultsmentioning

confidence: 99%

Section: Segmentation Algorithmmentioning

confidence: 99%

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Ceramic‐hydrogel composite as carrier for cold‐plasma reactive‐species: Safety and osteogenic capacity in vivo

Solé‐Martí

Labay

Raymond

et al. 2022

Plasma Processes & Polymers

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“…3D-printing technologies have enabled the production of scaffolds with greater spatial resolution than traditional fabrication methods, providing complex porous hierarchical structures 72 – 75 . Moreover, the development of biomimetic self-setting ceramic inks has circumvented the problems associated with shrinkage in ceramic parts and has allowed nanostructured calcium phosphate scaffolds to be obtained 76 .…”

Section: Scaffolds For Bone Tissue Engineeringmentioning

confidence: 99%

Scaffold-based bone tissue engineering in microgravity: potential, concerns and implications

Mochi¹,

Scatena²,

Rodríguez

et al. 2022

npj Microgravity

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One of humanity’s greatest challenges is space exploration, which requires an in-depth analysis of the data continuously collected as a necessary input to fill technological gaps and move forward in several research sectors. Focusing on space crew healthcare, a critical issue to be addressed is tissue regeneration in extreme conditions. In general, it represents one of the hottest and most compelling goals of the scientific community and the development of suitable therapeutic strategies for the space environment is an urgent need for the safe planning of future long-term manned space missions. Osteopenia is a commonly diagnosed disease in astronauts due to the physiological adaptation to altered gravity conditions. In order to find specific solutions to bone damage in a reduced gravity environment, bone tissue engineering is gaining a growing interest. With the aim to critically investigate this topic, the here presented review reports and discusses bone tissue engineering scenarios in microgravity, from scaffolding to bioreactors. The literature analysis allowed to underline several key points, such as the need for (i) biomimetic composite scaffolds to better mimic the natural microarchitecture of bone tissue, (ii) uniform simulated microgravity levels for standardized experimental protocols to expose biological materials to the same testing conditions, and (iii) improved access to real microgravity for scientific research projects, supported by the so-called democratization of space.

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“…Raymond et al . [ 249 ] reported that α-TCP scaffolds by direct ink writing presented more crystallization nanostructures, and had a major impact on permeability and protein adsorption capacity after hydrothermal processes when compared with biomimetic treatment.…”

Section: Functional Engineering Strategies Of Ceramic Materials For H...mentioning

confidence: 99%

Functional engineering strategies of 3D printed implants for hard tissue replacement

Chen

Huang

Liu

et al. 2022

Regenerative Biomaterials

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3D printing technology with the rapid development of printing materials are widely recognized as a promising way to fabricate bioartificial bone tissues. In consideration of the disadvantages of bone substitutes, including poor mechanical properties, lack of vascularization and insufficient osteointegration, functional modification strategies can provide multiple functions and desired characteristics of printing materials, enhance their physicochemical and biological properties in bone tissue engineering. Thus, this review focuses on the advances of functional engineering strategies for 3D printed biomaterials in hard tissue replacement. It is structured as introducing 3D printing technologies, properties of printing materials (metals, ceramics and polymers) and typical functional engineering strategies utilized in the application of bone, cartilage and joint regeneration.

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Hydrothermal processing of 3D-printed calcium phosphate scaffolds enhances bone formation in vivo: a comparison with biomimetic treatment

Cited by 15 publications

References 76 publications

Ceramic‐hydrogel composite as carrier for cold‐plasma reactive‐species: Safety and osteogenic capacity in vivo

Ceramic‐hydrogel composite as carrier for cold‐plasma reactive‐species: Safety and osteogenic capacity in vivo

Scaffold-based bone tissue engineering in microgravity: potential, concerns and implications

Functional engineering strategies of 3D printed implants for hard tissue replacement

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