Hydrothermal pretreatment of lignocellulosic biomass for hemicellulose recovery

Scapini, Thamarys; Santos, Maicon Sérgio Nascimento dos; Bonatto, Charline; Wancura, João H. C.; Mulinari, Jéssica; Camargo, Aline Frumi; Klanovicz, Natalia; Zabot, Giovani L.; Tres, Marcus V.; Fongaro, Gislaine; Treichel, Helen

doi:10.1016/j.biortech.2021.126033

Cited by 97 publications

(31 citation statements)

References 132 publications

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“…To apply the developed platform for actual lignocellulose conversion, further fermentation studies with biomass hydrolysates are warranted. Potentially, its performance could change with actual hydrolysates, since they are known to contain diverse growth-inhibiting compounds, such as furfural, 5-hydroxymethylfurfural, and levulinic acid, which originated from the degradation of sugars during acid/heat treatments [ 62 , 63 ]. This issue could be overcome by optimizing pretreatment to minimize the formation of toxic compounds and maximize sugar yields, which have been actively investigated [ 64 – 66 ].…”

Section: Discussionmentioning

confidence: 99%

A Vibrio-based microbial platform for accelerated lignocellulosic sugar conversion

Woo

Lim

Han

et al. 2022

Biotechnol Biofuels

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Background Owing to increasing concerns about climate change and the depletion of fossil fuels, the development of efficient microbial processes for biochemical production from lignocellulosic biomass has been a key issue. Because process efficiency is greatly affected by the inherent metabolic activities of host microorganisms, it is essential to utilize a microorganism that can rapidly convert biomass-derived sugars. Here, we report a novel Vibrio-based microbial platform that can rapidly and simultaneously consume three major lignocellulosic sugars (i.e., glucose, xylose, and arabinose) faster than any previously reported microorganisms. Results The xylose isomerase pathway was constructed in Vibrio sp. dhg, which naturally displays high metabolic activities on glucose and arabinose but lacks xylose catabolism. Subsequent adaptive laboratory evolution significantly improved xylose catabolism of initial strain and led to unprecedently high growth and sugar uptake rate (0.67 h−1 and 2.15 g gdry cell weight−1 h−1, respectively). Furthermore, we achieved co-consumption of the three sugars by deletion of PtsG and introduction of GalP. We validated its superior performance and applicability by demonstrating efficient lactate production with high productivity (1.15 g/L/h) and titer (83 g/L). Conclusions In this study, we developed a Vibrio-based microbial platform with rapid and simultaneous utilization of the three major sugars from lignocellulosic biomass by applying an integrated approach of rational and evolutionary engineering. We believe that the developed strain can be broadly utilized to accelerate the production of diverse biochemicals from lignocellulosic biomass.

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Section: Discussionmentioning

confidence: 99%

A Vibrio-based microbial platform for accelerated lignocellulosic sugar conversion

Woo

Lim

Han

et al. 2022

Biotechnol Biofuels

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“…41,42 Hemicelluloses are endowed with lower chemical and thermal stability than cellulose, allowing it to be chemically modified more easily. 43,44 Owing to the abundant polar -OH groups, hemicellulose presents high hydrophilicity. To make it hydrophobic, a hydrophobic lauric acid was grafted to -OH at C2, C3 of hemicellulose via esterification modification to prepare hemicellulose-graft-lauric acid (H-LA).…”

Section: Fabrication Of H-la-wax Pickering Emulsionsmentioning

confidence: 99%

All-biomass-based eco-friendly waterproof coating for paper-based green packaging

et al. 2022

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“…[8] Nevertheless, each pretreatment has its shortcomings, such as the strong corrosion of dilute acid, high energy demand of steam explosion and hydrothermal pretreatments, and high volatility of organic solvents for organosolv pretreatment. [7] Subsequently, ionic liquids were applied for the pretreatment due to their high thermostability and delignification efficiency. [9] Currently, deep eutectic solvent (DES) pretreatment has been significantly sought after as a promising pretreatment technology for its lower environmental and economic impacts as well as its high delignification efficiency.…”

Section: Introductionmentioning

confidence: 99%

“…However, the heterogeneity and structural complexity of the cell wall has hindered the efficient utilization of lignocellulosic biomass [2] . To overcome this hindrance, a variety of pretreatment technologies have been developed, such as acid (dilute sulfuric acid), [3] alkali (sodium hydroxide, ammonia), [4] ammonia fiber explosion, [5] reductive catalytic fractionation, [6] hydrothermal, [7] and organosolv pretreatment [8] . Nevertheless, each pretreatment has its shortcomings, such as the strong corrosion of dilute acid, high energy demand of steam explosion and hydrothermal pretreatments, and high volatility of organic solvents for organosolv pretreatment [7] .…”

Section: Introductionmentioning

confidence: 99%

“…To overcome this hindrance, a variety of pretreatment technologies have been developed, such as acid (dilute sulfuric acid), [3] alkali (sodium hydroxide, ammonia), [4] ammonia fiber explosion, [5] reductive catalytic fractionation, [6] hydrothermal, [7] and organosolv pretreatment [8] . Nevertheless, each pretreatment has its shortcomings, such as the strong corrosion of dilute acid, high energy demand of steam explosion and hydrothermal pretreatments, and high volatility of organic solvents for organosolv pretreatment [7] . Subsequently, ionic liquids were applied for the pretreatment due to their high thermostability and delignification efficiency [9] .…”

Section: Introductionmentioning

confidence: 99%

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Unveiling the Migration and Transformation Mechanism of Lignin in Eucalyptus During Deep Eutectic Solvent Pretreatment

Hong

Shen

et al. 2022

ChemSusChem

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Deep eutectic solvents (DESs) have unique advantages in biomass conversion. However, the migration and transformation mechanism of lignin in the cell wall during the DES pretreatment is still elusive. In this work, Eucalyptus blocks were pretreated in choline chloride/lactic acid DES to reveal the lignin migration. Meanwhile, the remaining lignin in the pretreated residue, the regenerated DES lignin, and the solubilized degraded lignin in the recovered DES were investigated to decipher the lignin transformation. Results showed that the DES pretreatment resulted in the penetration of DES from the cell lumen to the cell wall, and lignin in the secondary wall was more easily dissolved than that in the cell corner middle lamella. The syringyl unit of lignin was better stabilized in the DES than the guaiacyl unit of lignin. The condensed lignin fraction mainly remained in the pretreated residue, while the solubilized degraded lignin fraction was monomeric aromatic ketone compounds. This study elucidates the fate of lignin during the DES pretreatment, which could also promote the development of a modern lignocellulosic pretreatment technique.

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Hydrothermal pretreatment of lignocellulosic biomass for hemicellulose recovery

Cited by 97 publications

References 132 publications

A Vibrio-based microbial platform for accelerated lignocellulosic sugar conversion

A Vibrio-based microbial platform for accelerated lignocellulosic sugar conversion

All-biomass-based eco-friendly waterproof coating for paper-based green packaging

Unveiling the Migration and Transformation Mechanism of Lignin in Eucalyptus During Deep Eutectic Solvent Pretreatment

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