Hydrothermal Extraction and Micronization of Polysaccharides from Ganoderma lucidum in a One-Step Process

Matsunaga, Yui; Diono, Wahyu; Machmudah, Siti; Askin, Ruhan; Quitain, Armando T.; Sasaki, Masahiro; Goto, Motonobu

doi:10.15376/biores.8.1.461-471

Cited by 5 publications

(3 citation statements)

References 36 publications

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“…Besides, organic solvent remains in the product and may have harmful effects especially if the products are intended for human consumption. As alternative methods, we have been investigating the application of two environmentally benign pressurized fluid technologies, i.e., supercritical carbon dioxide (SCCO 2 ) − and hydrothermal extraction, − for the isolation of bioactive compounds from various natural products, including seaweeds.…”

Section: Introductionmentioning

confidence: 99%

Microwave–Hydrothermal Extraction and Degradation of Fucoidan from Supercritical Carbon Dioxide Deoiled Undaria pinnatifida

Quitain

Kai

Sasaki

et al. 2013

Ind. Eng. Chem. Res.

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Marine algae, such as Undaria pinnatifida, commonly known as “wakame” in Japan, contain valuable bioactive organic compounds including lipids and polysaccharides. However, substandard seaweeds that do not meet strict quality standards are normally discarded as wastes or returned to the sea, a situation which is becoming a serious environmental concern. In this work, hydrothermal treatment of the supercritical carbon dioxide deoiled wakame was investigated using microwave and conventional heating to recover and degrade Undaria polysaccharides (i.e., fucoidan) into highly potent low-molecular-weight components of about 5–30 kDa. Results showed advantages of microwave heating compared to conventional heating, obtaining the target molecular weight at a temperature close to 140 °C. Continuous microwave irradiation at constant microwave power in a short irradiation time of 1 min also gave promising results.

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Section: Introductionmentioning

confidence: 99%

Microwave–Hydrothermal Extraction and Degradation of Fucoidan from Supercritical Carbon Dioxide Deoiled Undaria pinnatifida

Quitain

Kai

Sasaki

et al. 2013

Ind. Eng. Chem. Res.

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“…About 17 amino acids link to polysaccharide molecules. They are the polar amino acids that make the molecules soluble more easily when increasing temperature [11]. Therefore, at 80 o C, the extraction efficiency was the best.…”

Section: Discussionmentioning

confidence: 99%

Factors Affect on the Total Polysaccharide Content in Ganoderma Lucidum (Leyss EX.FR.) Karst, Ganodermataceae

Huynh

2023

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Background: Ganoderma lucidum is a precious remedy with the active ingredient polysaccharides effective in treating diseases such as diabetes, hepatitis, cancer, etc. During cultivation, factors such as harvest time, temperature, and humidity affect the obtained polysaccharide content. At the same time, in the extraction process, the polysaccharide content is also affected by the material/solvent ratio, extraction time, extraction temperature, and the number of repetitions of extraction. Objectives: To determine the growing conditions and the time of harvesting and extracting conditions of Ganoderma lucidum with the highest polysaccharide content. Materials and methods: Using phenol-sulfuric acid reagent (concentrated) to determine the polysaccharide content, the growth stage and extraction conditions leading to the most polysaccharide content. Results: The highest polysaccharide content was obtained in mushrooms on the 75th day, based on bagasse with growing environments from the 1st to the 34th day: temperature of 25-30⁰C and humidity of 60-70%; that from the 35th to the 67th day: temperature of 22-28 ⁰C and humidity of 80-90%; that from the 68th to the 75th day: temperature of 22-28⁰C and humidity of 60-70%. The best extraction conditions: medicinal/solvent ratio: 1/40, temperature: 80⁰C, time: 90 minutes, and the number of extractions: 3 times. Conclusion: Ganoderma lucidum should be grown, harvested, and extracted under the above conditions to obtain the highest polysaccharide content.

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“…The weaknesses of HWE, including high energy consumption and low yields, had inhibited this method from being widely applied. To optimize the extraction technology, many novel techniques had been employed, such as ultrasonic-assisted extraction (UAE), enzyme-assisted extraction (EAE), supercritical fluid extraction (SFE), and pressurized liquid extraction (PLE) (Kadam et al 2013;Matsunaga et al 2013;Ye et al 2015). These novel extraction technologies had been successfully used in food and pharmaceutical applications for extraction of bioactive compounds (Easmin et al 2015).…”

Section: Introductionmentioning

confidence: 99%

Microwave-assisted Extraction of Polysaccharides from Bamboo (Phyllostachys acuta) Leaves and their Antioxidant Activity

Zhang

Zhong

et al. 2016

BioResources

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Polysaccharides were isolated from Phyllostachys acuta leaves by microwave-assisted extraction under various temperatures and time. The obtained polysaccharides were characterized by acid hydrolysis, the FolinCiocalteu method, and Fourier transform infrared spectroscopy (FTIR). The major monosaccharides presented in the extracts were arabinose (258.0 mg/g to 414.5 mg/g), galactose (167.0 mg/g to 289.2 mg/g), and glucose (157.4 mg/g to 246.7 mg/g) along with some mannose, fructose, and xylose. The total phenol yield of the bamboo leaves was 0.31 mg/g to 0.73 mg/g. The FTIR spectra revealed that the polysaccharides mostly consisted of β-glycosidic linkages. For the cytotoxicity, the presence of polysaccharides considerably elevated the multiplication of HepG2 cells and showed no growth inhibition for the samples. For the antioxidant activities, the polysaccharides exhibited excellent abilities both in the diphenyl picrylhydrazyl radical potential (DPPH) assay and ferric reducing antioxidant potential (FRAP) assay. The results suggest that bamboo leaf polysaccharides have great potential to be applied in the food, healthcare, and pharmaceutical fields.

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Hydrothermal Extraction and Micronization of Polysaccharides from Ganoderma lucidum in a One-Step Process

Cited by 5 publications

References 36 publications

Microwave–Hydrothermal Extraction and Degradation of Fucoidan from Supercritical Carbon Dioxide Deoiled Undaria pinnatifida

Microwave–Hydrothermal Extraction and Degradation of Fucoidan from Supercritical Carbon Dioxide Deoiled Undaria pinnatifida

Factors Affect on the Total Polysaccharide Content in Ganoderma Lucidum (Leyss EX.FR.) Karst, Ganodermataceae

Microwave-assisted Extraction of Polysaccharides from Bamboo (Phyllostachys acuta) Leaves and their Antioxidant Activity

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