Hydrotalea sandarakina sp. nov., isolated from a hot spring runoff, and emended descriptions of the genus Hydrotalea and the species Hydrotalea flava

Abstract

Hydrotalea sandarakina sp. nov., isolated from a hot spring runoff, and emended descriptions of the genus Hydrotalea and the species Hydrotalea flava Two bacterial isolates, designated AF-51 T and AF-50, with an optimum growth temperature of about 45 6C and an optimum pH for growth between 6.0 and 6.5, were recovered from a hot spring in the Furnas, Á rea da Fonte 1825, on the Island of Sã o Miguel in the Azores. Based on 16S rRNA gene sequence analysis, these strains were related most closely to the type strain of Hydrotalea flava at a pairwise similarity of 95.7 %. The two strains were orange-pigmented and formed non-motile, rod-shaped cells that stained Gram-negative and were aerobic and oxidaseand catalase-positive. The major fatty acids were iso-C 15 : 0 , iso-C 17 : 0 3-OH and iso-C 16 : 0 . The major respiratory quinone was menaquinone 7. Based on phylogenetic, physiological and biochemical characteristics, these strains from the Azores are considered to represent a single novel species of the genus Hydrotalea, for which the name Hydrotalea sandarakina sp. nov. is proposed. The type strain is AF-51 T (5DSM 23241 T 5LMG 25526 T ). We provide emended descriptions of the genus Hydrotalea and of H. flava to reflect new results obtained in this study.

Complete genome sequence of DSM 30083T, the type strain (U5/41T) of Escherichia coli, and a proposal for delineating subspecies in microbial taxonomy

“…The G+C content of 50.6% inferred from the genome sequence is in agreement with the value of 50.7 ± 0.6 mol% determined for strain DSM 30083 T by Albuquerque et al [ 101 ], but differs slightly from the G+C content of 51.0-51.7 mol%, determined from deposit ATCC 11775 T [ 29 ]. The G+C content range of E. coli strains was reported as 48.5-52.1 mol% [ 29 ], in conflict with more recent results [ 26 ].…”

Analysis of 1,000 Type-Strain Genomes Improves Taxonomic Classification of Bacteroidetes

“…When H. penzbergensis was proposed, V. magnilacihabitans was not considered; as shown in Table 3, H. penzbergensis is a later heterotypic synonym of V. magnilacihabitans . These two genera as well as Asinibacterium (Lee D.-G. et al, 2013) and even Hydrotalea (Kämpfer et al, 2011b; Albuquerque et al, 2012) and Parasediminibacterium (Kang et al, 2016) display phenotypic features similar to those of Sediminibacterium , the only known differences being presence or absence of motility, which may well be homoplastic given the findings reported above (Table 1), and a negative response for oxidase and catalase activities reported for Asinibacterium (Supplementary Table 1). Consequently, it is proposed that Asinibacterium lactis and Vibrionimonas magnilacihabitans be classified within Sediminibacterium .…”

“…The description is as before (Albuquerque et al, 2012) with the following modification. The genomic G+C content is c. 32–40%, the genome size is c. 3.0–3.7 Mbp.…”

Complete Genome Sequence for Asinibacterium sp. Strain OR53 and Draft Genome Sequence for Asinibacterium sp. Strain OR43, Two Bacteria Tolerant to Uranium

“…The type strain Asinibacterium lactis was isolated from donkey milk powder (1). Closely related genera include Sediminibacterium , Vibrionimonas , and Hydrotalea (2–6). Related sequences (16S rRNA) were detected ubiquitously in the environment but most notably in sites contaminated with hydrocarbons, heavy metals, and/or radionucleotides (7–14).…”